LOCUS       sequence-233130-       10033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of ilux for bioluminescence imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Gregor C, Gwosch KC, Sahl SJ, Hell SW
  TITLE     Strongly enhanced bacterial bioluminescence with the ilux operon for
            single-cell imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 
            10.1073/pnas.1715946115. Epub 2018 Jan 16.
  PUBMED    29339494
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1715946115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-01-30- 30"; volume: "115"; issue: "5"; pages: "962-967"
COMMENT     Sequence Label: ilux pQE(-)
FEATURES             Location/Qualifiers
     source          1..10033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(10..24,25..30,31..47,48..54)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             121..1563
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIITGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHDIVNFLYTVGQRWKNDEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGPKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWTIIESDAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1576..2499
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2554..3636
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMERLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGADMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESAATTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3651..4634
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINPTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPAEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNEDFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4819..5931
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      6780..6874
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6918..7577
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6984..7003)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      7642..7728
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7775..7797)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7883..8023
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7938..7957
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8038..8055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8209..8797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8289..8308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8968..9759,9760..9828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9591..9610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9829..9933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10001..10019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactggatcc
      121 atgactaaaa aaatttcatt cattattacc ggccaggttg aaatctttcc cgaaagcgat
      181 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
      241 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcatgacatt
      301 gtcaattttc tctatacggt agggcaaaga tggaaaaatg atgaatactc aagacgcagg
      361 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
      421 gccaattgga tatctatgat attatgttct aaaggcggcc tttatgatgt tgtagaaaat
      481 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
      541 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
      601 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
      661 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
      721 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
      781 gaaattatgc gacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
      841 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggtcctaa aaagagtctt
      901 tgcattatcg ataatcctgt tgatttgacg tctgcagcga caggtgcggc tcatgatgtt
      961 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattatat gggaaatcat
     1021 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
     1081 aatgcaaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     1141 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggacgattat tgagtcagat
     1201 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
     1261 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
     1321 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
     1381 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
     1441 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
     1501 gcggttgaaa tagaacagac tcgtttcctg gaagaagata agttccttgt atttgtccca
     1561 taataggtaa aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
     1621 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
     1681 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
     1741 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
     1801 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
     1861 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttca
     1921 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
     1981 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
     2041 ctcagtctac ccattaatga attgccgaat aatctagatt ttgaaggcca taaattgggt
     2101 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
     2161 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
     2221 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
     2281 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
     2341 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
     2401 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
     2461 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
     2521 actcaaatag caatataagg actctctgaa ttcatgaaat ttggaaactt tttacttaca
     2581 taccaacctc cccaattttc tcaaacagag gtaatggaac gtttggttaa attaggtcgc
     2641 atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt
     2701 ggtctacttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg
     2761 aatgtaggaa ccgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat
     2821 gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt
     2881 tacaacaagg actttcgcgt attcggcgcg gatatgaata acagtcgcgc cttagcggaa
     2941 tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa
     3001 catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg
     3061 gtttatgtgg tggctgaatc agctgcgacg actgagtggg cagctcaatt tggcctaccg
     3121 atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat
     3181 gaggtggctc aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata
     3241 acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat
     3301 tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt
     3361 tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat
     3421 cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac
     3481 ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct
     3541 aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca
     3601 tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa atgaaatttg
     3661 gattgttctt ccttaacttc atcaatccga caactgttca agaacaaagt atagttcgca
     3721 tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta gtgtatgaaa
     3781 atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt tttctgctcg
     3841 gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact catcatcctg
     3901 tccgcatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga tttattttag
     3961 ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg gctgaatatc
     4021 aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca acaggctatt
     4081 gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc catgcttata
     4141 cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt gagtgggcgg
     4201 ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt agatatgaat
     4261 atgctgaaag atataaagcc gtcgcggata aatatgacgt tgacctatca gagatagacc
     4321 atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa caagagacgc
     4381 gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaagatttc gaaaataaac
     4441 ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata actgcggcta
     4501 aattggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg
     4561 atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag aagtaccaca
     4621 tggaatatac ctaaccatgg tagatttcga gttgcagcga ggcggcaagt gaacgaatcc
     4681 ccaggagcat agataactat gtgactgggg tgagtgaaag cagccaacaa agcagcagct
     4741 tgaaagatga agggtataaa agagtatgac agcagtgctg ccatactttc taatattatc
     4801 ttgaggagta aaacaggtat gacttcatat gttgataaac aagaaattac agcaagctca
     4861 gaaattgatg atttgatttt ttcgagcgat ccattagtgt ggtcttacga cgagcaggaa
     4921 aaaatcagaa agaaacttgt gcttgatgca tttcgtaatc attataaaca ttgtcgagaa
     4981 tatcgtcact actgtcaggc acacaaagta gatgacaata ttacggaaat tgatgacata
     5041 cctgtattcc caacatcggt ttttaagttt actcgcttat taacttctca ggaaaacgag
     5101 attgaaagtt ggtttaccag tagcggcacg aatggtttaa aaagtcaggt ggcgcgtgac
     5161 agattaagta ttgagagact cttaggctct gtgagttatg gcatgaaata tgttggtagt
     5221 tggtttgatc atcaaataga attagtcaat ttgggaccag atagatttaa tgctcataat
     5281 atttggttta aatatgttat gagtttggtg gaattgttat atcctacgac atttaccgta
     5341 acagaagaac gaatagattt tgttaaaaca ttgaatagtc ttgaacgaat aaaaaatcaa
     5401 gggaaagatc tttgtcttat tggttcgcca tactttattt atttactctg ccattatatg
     5461 aaagataaaa aaatctcatt ttctggagat aaaagccttt atatcataac cggaggcggc
     5521 tggaaaagtt acgaaaaaga atctctgaaa cgtgatgatt tcaatcatct tttatttgat
     5581 actttcaatc tcagtgatat tagtcagatc cgagatatat ttaatcaagt tgaactcaac
     5641 acttgtttct ttgaggatga aatgcagcgt aaacatgttc cgccgtgggt atatgcgcga
     5701 gcgcttgatc ctgaaacgtt gaaacctgta cctgatggaa cgccggggtt gatgagttat
     5761 atggatgcgt cagcaaccag ttatccagca tttattgtta ccgatgatgt cgggataatt
     5821 agcagagaat atggtaagta tcccggcgtg ctcgttgaaa ttttacgtcg cgtcaatacg
     5881 aggacgcaga aagggtgtgc tttaagctta accgaagcgt ttgatagttg agtcgaccta
     5941 aggagaaaga aatggtgaag atacagccca tccccacaac tagccagggc agccttttta
     6001 taatgaatag caccatagag acaatcctgg gccatagatc cattaggaag ttcacatctg
     6061 aacctattgc tagtgagcag ctgcaaacga ttcttcagtc tgggctcgct gcttcaagct
     6121 catccatgct gcaggttgtg agtataattc gggttacaga cacggaaaag agaaaattgc
     6181 tcgctcaata tgccggcaac cagacgtatg tggaatccgc tgctgagttc ctggtctttt
     6241 gtatagacta ccagcgacac gctactatca accccgatgt ccaagctgac tttaccgagc
     6301 tgaccctgat tggtgcagtg gattccggca taatggccca gaattgcctc ctggcagcag
     6361 aatcaatggg tcttggcgga gtctatatcg gaggacttcg gaactcagct gcccaagtgg
     6421 atgagttgct cggactgccc aagaacacag ctatcctctt cggaatgtgc ttggggcacc
     6481 ccgatcagag ccctgagaca aagcctagac tgcccgctca tgtgatcgtg cacgagaacc
     6541 aatatcaagc tctgaacatt gacgacgtac aggcgtatga caaaacattg caggagtatt
     6601 atgccagcag aaccagcaac cagaagcaga gtgtctggtc ccaggaaact gcaggcaagc
     6661 tggccggaga atccctccca cacatcctgc catacctgaa ctccaagggc cttgccagaa
     6721 ggtaactaag gagaaagaac ccgggtcgac ctgcagccaa gcttaattag ctgagcttgg
     6781 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
     6841 cggttgccgc cgggcgtttt ttattggtga gaatccaagc tagcttggcg agattttcag
     6901 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     6961 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     7021 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     7081 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     7141 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     7201 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     7261 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     7321 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     7381 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     7441 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     7501 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     7561 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggggtaa
     7621 tgactctcta gcttgaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     7681 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccctctaga
     7741 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7801 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7861 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     7921 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7981 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     8041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8641 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9961 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10021 ctttcgtctt cac
//
