LOCUS       sequence-233138-       12836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a dominant negative Kash construct that disrupts LINC 
            complexes in addition to fluorescently tagged (mRuby2) 
            alpha-Actinin-2 on an adenoviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Seelbinder B, Ghosh S, Schneider SE, Scott AK, Berman AG, Goergen 
            CJ, Margulies KB, Bedi KC Jr, Casas E, Swearingen AR, Brumbaugh J, 
            Calve S, Neu CP
  TITLE     Nuclear deformation guides chromatin reorganization in cardiac 
            development and disease.
  JOURNAL   Nat Biomed Eng. 2021 Dec;5(12):1500-1516. doi: 
            10.1038/s41551-021-00823-9. Epub 2021 Dec 2.
  PUBMED    34857921
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-021-00823-9"; journalName: "Nat Biomed Eng"; date: 
            "2021-12"; volume: "5"; issue: "12"; pages: "1500-1516"
COMMENT     Sequence Label: Ad:2CMV_A2R2-N25K3
FEATURES             Location/Qualifiers
     source          1..12836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        398..701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        702..905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1027..1758
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    2144..2265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2363..2666
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2667..2870
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             5612..6322
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    6484..6605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   9796..9898
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(9987..10004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10158..10746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10238..10257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11578..12372
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11578..12372
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11632..11651)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12242..12261
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(12420..12799)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12482..12503)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12694..12713
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat actgtaatag taatcaatta
      361 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      481 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      541 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      601 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      661 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      721 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      781 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      841 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      901 gagctggttt agtgaaccgt cagatccgct agagatctgg taccgtcgac gcggccgctc
      961 gacaccatga aactaggacg ggccgtgctg ggcctgctgc tgctggcgcc gtccgtggtg
     1021 caggcgatgg tgagcaaggg cgaggagctg atcaaggaga acatgcacac gaagctgtac
     1081 atggaaggca ccgtgaacaa ccaccacttc aagtgcaccc acgaagggga gggcaagccc
     1141 tacgagggca cccagaccaa caggattaag gtggtggagg gaggccccct gccgttcgca
     1201 ttcgacatcc tggccacctg ctttatgtac gggagcaaga ccttcatcaa ccacacccag
     1261 ggcatccccg atttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     1321 acatacgaag acgggggcgt gcttaccgtt acccaggaca ccagcctcca ggacggctgc
     1381 ttgatctaca acgtcaagct cagaggggtg aacttcccat ccaacggccc tgtgatgcag
     1441 aagaaaacac tcggctggga ggccagtacc gagaccctgt accccgctga cggcggcctg
     1501 gaaggcagat gcgacatggc cctgaagctc gtgggcgggg gccacctgca ctgcaacctg
     1561 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctacttt
     1621 gtggaccgca gactggaaag aatcaaggag gccgacaatg agacctacgt cgagcagcac
     1681 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaatggc
     1741 atggacgagc tgtacaaggg ggatccaccg gtcgccacca gtcggaggca gaagcggtgg
     1801 agtccctgct ctctcctaca gaaagcatgc cgtgtggcac tgccattgca gctgttgctc
     1861 ctgctctttc tgctgctgct gttcctgctg ccggccggcg aggaggagcg cagctgcgcc
     1921 ctggccaaca acttcgcccg ctcctttgcg ctcatgcttc ggtacaatgg ccccccgccc
     1981 acctagggta ccctaagctt ctagataaga tatccgatcc accggatcta gataactgat
     2041 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2101 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg gatctgggcg
     2281 tggttaatat ttcatagcgc gtaatactgt aatagtaatc aattacgggg tcattagttc
     2341 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2401 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2461 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2521 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2581 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2641 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2701 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2761 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2821 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     2881 accgtcagat ccgctagagg ctagcaccat gaatcagata gagcccggcg tgcagtacaa
     2941 ctatgtctac gacgaagatg agtatatgat ccaggaggag gagtgggacc gcgacctgct
     3001 cctcgacccg gcctgggaga agcagcagag gaagacgttc actgcctggt gcaactcaca
     3061 cctgcggaaa gctggcaccc agatcgagaa catcgaggag gatttcagga acggcctcaa
     3121 gctcatgcta cttctagaag tcatctcagg ggaaaggctg cccaaacctg accggggaaa
     3181 aatgcggttc cacaagattg cgaatgtcaa caaggctctg gattacatag ccagcaaggg
     3241 agtgaagctg gtatctattg gagctgaaga aatagtcgat ggcaatgtga agatgaccct
     3301 gggtatgatc tggaccatca tcctccgctt cgccattcag gacatctcag tggaagaaac
     3361 atctgccaaa gaagggctgc tgctatggtg tcagaggaag acagctccat acagaaatgt
     3421 gaatatccag aacttccaca ccagctggaa agatggcctt ggactctgtg ccctcatcca
     3481 cagacaccgg cccgacctca ttgactactc aaagcttaac aaggatgacc ccataggaaa
     3541 cattaacctg gccatggaaa tagcagagaa gcacttggat atccctaaga tgttggatgc
     3601 tgaagacatt gtcaacactc ccaaacccga tgaaagagcc atcatgactt acgtttcctg
     3661 cttctaccat gctttcgcgg gagcggagca ggccgagaca gcagctaaca gaatatgtaa
     3721 ggttcttgct gtgaatcaag agaatgagag gctgatggaa gaatatgaga ggctagcgag
     3781 tgagctgttg gagtggattc ggcgcacaat cccctggctg gagaacagga cgcctgagaa
     3841 gaccatgcag gccatgcaga agaagctgga ggacttccgg gattaccgtc gtaagcataa
     3901 gccccccaag gtgcaggaga agtgccagct ggagatcaac ttcaacacgc tgcagaccaa
     3961 gcttcggatc agcaaccggc ccgccttcat gccctccgag ggcaagatgg tgtcggacat
     4021 tgctggtgcc tggcagaggt tggagcaggc tgagaagggc tatgaggagt ggctattgaa
     4081 tgagattcga agactggagc gcttggagca cttggctgag aagttcaggc agaaggcctc
     4141 cacgcacgag acatgggctt atggcaaaga acagatcttg ctgcagaagg actacgagtc
     4201 tgcgtccctg acggaagttc gagccctact tcggaagcac gaggcctttg agagcgacct
     4261 ggctgcacac caggaccgcg tggagcagat cgctgccatt gcgcaggagc tcaatgaact
     4321 ggactatcac gatgctgtga acgtcaatga tcggtgccag aaaatttgcg accagtggga
     4381 tagattgggg acgctcaccc agaagaggag ggaggccttg gagagaactg agaagttgct
     4441 ggaaacaatc gaccaactgc acctggagtt tgccaagaga gccgcgccct tcaacaactg
     4501 gatggaggga gccatggagg acctgcagga catgttcatt gtccacagca tcgaggagat
     4561 ccagagtctg atcactgcac atgagcagtt caaggccaca ctgcccgagg ccgacgggga
     4621 gcggcagtcc atcctggcca tccagaacga ggtggagaag gtgatccaga gctacagcat
     4681 ccggatcagc tccagcaacc cctacagcac tgtcaccatg gatgagctgc gcaacaagtg
     4741 ggacaaggtg aagcagctgg tgccggtccg ggatcagtcc ctgcaggagg agctggctcg
     4801 ccaacatgct aatgagcgtc tgcggcgcca gtttgctgct caggccaatg ccattgggcc
     4861 ctggatccag aacaagatgg aggagatcgc tcggagctcc atccagatca ccggggccct
     4921 ggaggaccag atgaaccagc tgaagcagta cgaacacaac atcatcaact acaagaacaa
     4981 catcgacaag ctggagggcg accaccagct catccaggag gccctggtct tcgacaacaa
     5041 gcacacgaac tacaccatgg agcatatccg tgtcggatgg gagttgctgc tgacaactat
     5101 cgccagaacc atcaatgagg tggagaccca gatcctgaca agagatgcca agggcatcac
     5161 ccaggagcag atgaatgagt tcagagcctc cttcaaccac tttgacagga ggaagaacgg
     5221 cctgatggat catgaggatt tcagagcctg cctcatttcc atgggctatg acttgggtga
     5281 agctgaattt gcccgcatta tgactttggt tgaccccaac ggacaaggca cggtcacctt
     5341 ccagtccttc attgacttca tgactagaga gaccgcagac accgacacgg cagagcaggt
     5401 catcgcctcc ttccggattc tggcttctga taagccttac atcttggcag aggagcttcg
     5461 tcgagagctg cctccggatc aggcccagta ctgcatcaag agaatgcccc catactcagg
     5521 cccgggcagt gtccccgggg ccctggacta cactgccttc tcctctgccc tctacgggga
     5581 aagcgacctc ggcgaccctc ctgttgcaac gatggtgtct aagggcgaag agctgatcaa
     5641 ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg
     5701 cacaggtgaa ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat
     5761 cgagggagga cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag
     5821 ccgtactttt atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga
     5881 gggttttact tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca
     5941 ggacaccagc cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt
     6001 tccctccaat ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat
     6061 gatgtatcca gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg
     6121 tggtggccat ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa
     6181 catcaagatg cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga
     6241 caatgaaatg ttcgtagtac aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg
     6301 tgggatggac gagctgtaca agtaactcga gcctaagctt ctagataaga tatccgatcc
     6361 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     6421 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6481 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6601 tcttaacgcg gatctgggcg tggttaaggg tgggaaagaa tatataaggt gggggtctta
     6661 tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagcaccaac tcgtttgatg
     6721 gaagcattgt gagctcatat ttgacaacgc gcatgccccc atgggccggg gtgcgtcaga
     6781 atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc cgcaaactct actaccttga
     6841 cctacgagac cgtgtctgga acgccgttgg agactgcagc ctccgccgcc gcttcagccg
     6901 ctgcagccac cgcccgcggg attgtgactg actttgcttt cctgagcccg cttgcaagca
     6961 gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac ggctcttttg gcacaattgg
     7021 attctttgac ccgggaactt aatgtcgttt ctcagcagct gttggatctg cgccagcagg
     7081 tttctgccct gaaggcttcc tcccctccca atgcggttta aaacataaat aaaaaaccag
     7141 actctgtttg gatttggatc aagcaagtgt cttgctgtct ttatttaggg gttttgcgcg
     7201 cgcggtaggc ccgggaccag cggtctcggt cgttgagggt cctgtgtatt ttttccagga
     7261 cgtggtaaag gtgactctgg atgttcagat acatgggcat aagcccgtct ctggggtgga
     7321 ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt gtagatgatc cagtcgtagc
     7381 aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag caagctgatt gccaggggca
     7441 ggcccttggt gtaagtgttt acaaagcggt taagctggga tgggtgcata cgtggggata
     7501 tgagatgcat cttggactgt atttttaggt tggctatgtt cccagccata tccctccggg
     7561 gattcatgtt gtgcagaacc accagcacag tgtatccggt gcacttggga aatttgtcat
     7621 gtagcttaga aggaaatgcg tggaagaact tggagacgcc cttgtgacct ccaagatttt
     7681 ccatgcattc gtccataatg atggcaatgg gcccacgggc ggcggcctgg gcgaagatat
     7741 ttctgggatc actaacgtca tagttgtgtt ccaggatgag atcgtcatag gccattttta
     7801 caaagcgcgg gcggagggtg ccagactgcg gtataatggt tccatccggc ccaggggcgt
     7861 agttaccctc acagatttgc atttcccacg ctttgagttc agatgggggg atcatgtcta
     7921 cctgcggggc gatgaagaaa acggtttccg gggtagggga gatcagctgg gaagaaagca
     7981 ggttcctgag cagctgcgac ttaccgcagc cggtgggccc gtaaatcaca cctattaccg
     8041 gctgcaactg gtagttaaga gagctgcagc tgccgtcatc cctgagcagg ggggccactt
     8101 cgttaagcat gtccctgact cgcatgtttt ccctgaccaa atccgccaga aggcgctcgc
     8161 cgcccagcga tagcagttct tgcaaggaag caaagttttt caacggtttg agaccgtccg
     8221 ccgtaggcat gcttttgagc gtttgaccaa gcagttccag gcggtcccac agctcggtca
     8281 cctgctctac ggcatctcga tccagcatat ctcctcgttt cgcgggttgg ggcggctttc
     8341 gctgtacggc agtagtcggt gctcgtccag acgggccagg gtcatgtctt tccacgggcg
     8401 cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg tgcgctccgg gctgcgcgct
     8461 ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag cgctgccggt cttcgccctg
     8521 cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc agcccctccg cggcgtggcc
     8581 cttggcgcgc agcttgccct tggaggaggc gccgcacgag gggcagtgca gacttttgag
     8641 ggcgtagagc ttgggcgcga gaaataccga ttccggggag taggcatccg cgccgcaggc
     8701 cccgcagacg gtctcgcatt ccacgagcca ggtgagctct ggccgttcgg ggtcaaaaac
     8761 caggtttccc ccatgctttt tgatgcgttt cttacctctg gtttccatga gccggtgtcc
     8821 acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca gacttgagag ggagtttaaa
     8881 cgaattcaat agcttgttgc atgggcggcg atataaaatg caaggtgctg ctcaaaaaat
     8941 caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc
     9001 aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg
     9061 gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct
     9121 tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc
     9181 gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg
     9241 agtcataatg taagactcgg taaacacatc aggttgattc acatcggtca gtgctaaaaa
     9301 gcgaccgaaa tagcccgggg gaatacatac ccgcaggcgt agagacaaca ttacagcccc
     9361 cataggaggt ataacaaaat taataggaga gaaaaacaca taaacacctg aaaaaccctc
     9421 ctgcctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc
     9481 agccataaca gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac
     9541 acggcaccag ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat
     9601 aggactaaaa aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg
     9661 aacctacgcc cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt
     9721 tttcccacgt tacgtcactt cccattttaa gaaaactaca attcccaaca catacaagtt
     9781 actccgccct aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac
     9841 tccaccccct cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatgtt
     9901 aattaacatg catggatcca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9961 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10021 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10081 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10141 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10321 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10561 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10921 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10981 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11041 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11101 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11161 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11221 gttgccattg ctgcagccat gagattatca aaaaggatct tcacctagat ccttttcacg
    11281 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
    11341 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
    11401 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
    11461 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
    11521 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
    11581 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
    11641 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
    11701 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
    11761 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
    11821 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    11881 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    11941 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    12001 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    12061 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
    12121 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    12181 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    12241 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    12301 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    12361 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc
    12421 cgaaatcggc accatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
    12481 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
    12541 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttgtg
    12601 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
    12661 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
    12721 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat
    12781 gcgccgctac agggcgcgtc cattcgccat tcaggatcga attaattctt aattaa
//
