LOCUS       sequence-233144-        7800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus delivery for stable expression of Lb crRNA, has LbDR; 
            Cloning vector for expression of LbCas12a crRNA. It contains BsmBI 
            site for cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler 
            L, Cobos Galvez A, Tschaharganeh DF
  TITLE     Multiplexed orthogonal genome editing and transcriptional activation
            by Cas12a.
  JOURNAL   Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. 
            Epub 2018 Dec 17.
  PUBMED    30559432
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0262-1"; journalName: "Nat Methods"; date: 
            "2019-01"; volume: "16"; issue: "1"; pages: "51-54"
COMMENT     Sequence Label: pMB30
FEATURES             Location/Qualifiers
     source          1..7800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1720..1837
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(1912..2160)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(1970..1989)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2140..2160)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2194..2405
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2431..2433,2434..2436,2437..3147)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2431..2433,2434..2436,2437..3147)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2476..2497)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2737..2756)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3084..3105
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3163..3219
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3658..4246
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3711..3731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4129..4140)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4318..4551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4629..4750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4666..4685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4720..4739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4790..4925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4945..4964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4946..4964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4974..4991)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4974..4990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4983..5005)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5132..5587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5219..5238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5429..5450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5613..5717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5718..5786,5787..6578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5936..5955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6749..7337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7238..7257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7491..7508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7625..7646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7661..7666,7667..7684,7685..7691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7704..7726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7723..7739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7723..7739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7758..7778
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7760..7778
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccaacggt tttaaaagaa aaggggggat
     1741 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1801 agaattacaa aaacaaatta caaaaattca aaattttcaa cttcggaatt cgtgtgctcg
     1861 agcaaaaaaa gagacgatat atcgtctcca tctacactta gtagaaatta cggtgtttcg
     1921 tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat
     1981 atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt
     2041 tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttctaattat
     2101 ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc
     2161 gggacccctc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2221 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     2281 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     2341 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     2401 cacaggtgtc gtgacgcggg atccgccacc atggtgagca agggcgagga gctgttcacc
     2461 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2521 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2581 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2641 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2701 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2761 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2821 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2881 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2941 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3001 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     3061 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3121 actctcggca tggacgagct gtacaaggcc gcaggcagcg gcgccaccaa cttcagcctg
     3181 ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaagacctt caacatctct
     3241 cagcaggacc ttgagctggt ggaagttgcc actgagaaaa tcaccatgct ctatgaggac
     3301 aacaagcacc atgttggtgc tgccatcagg accaagacag gagaaatcat ctctgctgtc
     3361 cacattgaag cctacattgg cagggtcact gtgtgtgcag aggccattgc cattgggtct
     3421 gcagtctcca atgggcagaa ggactttgac accattgtgg ctgtgaggca cccctacagt
     3481 gatgaggtgg acaggtccat cagagtggtg tccccctgtg gcatgtgcag ggagctcatc
     3541 tcagactatg cccctgattg ctttgttctg attgagatga atggcaagct ggtcaagaca
     3601 accattgagg agctgattcc actgaaatac accagaaact aaacgcgtta agtcgacaat
     3661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4021 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4201 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4261 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4321 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4381 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4441 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4501 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4561 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4621 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4741 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4921 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4981 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5041 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5101 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5161 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5221 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5281 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5341 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5401 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5461 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5521 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5581 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6241 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7501 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7621 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7741 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
//
