LOCUS       sequence-23316-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT TAP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             134..151
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             206..271
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    286..319
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(331..351)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        336..665
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      516..651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(675..694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cttcgtataa tgtatgctat acgaagttat ggatctaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ctctagcccg ggcggatccg aattcgcatg
      121 cgtcgacctc gagcatcacc atcaccatca cgattatgat attccaacta ctgctagcga
      181 gaatttgtat tttcagggtg agctcgacta caaagaccat gacggtgatt ataaagatca
      241 tgacatcgac tacaaggatg acgatgacaa gtagtctaga gatccataac ttcgtataat
      301 gtatgctata cgaagttatc tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
      361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      481 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      601 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      661 gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      721 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc a
//
