LOCUS       sequence-233161-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector encoding BFP-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  TITLE     Yusa CRISPR-Cas9 and mammalian expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKLV2-U6(BsmBI)-EF1a-BFP-BSD
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..38
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     30..47
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        391..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             607..787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    834..959
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1458..1691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1876..1920
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2218..2335
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2410..2658
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2410..2430
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2581..2600
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3192..4373
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3423..4365
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4322..4342
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4386..4395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4392..5093
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             5112..5165
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5172..5567
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5586..5639
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5730..5750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6148..6159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6611..6628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6617..6841
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6867..6887)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6867..6885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6906..6922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6906..6922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6919..6941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6930..6946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6954..6960,6961..6978,6979..6984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6999..7020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7137..7154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7308..7896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7388..7407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8067..8858,8859..8927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8690..8709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8928..9032)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9058..9513)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9195..9216)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9407..9426
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
       61 acgactcact atagggcgaa ttgactagta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
      601 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      661 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      721 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      781 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      841 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      901 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      961 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1021 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1081 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1141 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1201 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1261 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgcgct gatcttcaga
     1321 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1381 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1441 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1501 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1561 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1621 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1681 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1741 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1801 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1861 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1921 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1981 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2041 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2101 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2161 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     2221 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     2281 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     2341 ttattacagg gacagcagag atccagtttg gttagtaccg ggccctacgc gtccaaggtc
     2401 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2461 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2521 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2581 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2641 tggaaaggac gaaacaccgg agacgcatga ctcgtctcag tttaagagct atgctggaaa
     2701 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     2761 ggtgcttttt ttacgcgtga ggggcgcgga agccggggcg gaggaggagc caaccccggg
     2821 ctgggctgag acccgcagag gaagacgctc tagggatttg tcccggacta gcgagatggc
     2881 aaggctgagg acgggaggct gattgagagg cgaaggtaca ccctaatctc aatacaacct
     2941 ttggagctaa gccagcaatg gtagagggaa gattctgcac gtcccttcca ggcggcctcc
     3001 ccgtcaccac cccccccaac ccgccccgac cggagctgag agtaattcat acaaaaggac
     3061 tcgcccctgc cttggggaat cccagggacc gtcgttaaac tcccactaac gtagaaccca
     3121 gagatcgctg cgttcccgcc ccctcacccg cccgctctcg tcatcactga ggtggagaag
     3181 agcatgcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     3241 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaagtgg cgcggggtaa
     3301 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     3361 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     3421 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     3481 tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3541 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     3601 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     3661 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     3721 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     3781 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     3841 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     3901 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     3961 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     4021 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     4081 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     4141 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     4201 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     4261 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4321 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtggaagctt
     4381 ggcgcgccac catgggcagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4441 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4501 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     4561 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     4621 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     4681 acgaggacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     4741 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     4801 aaacactcgg ctgggaggcc ttcaccgaga ccctgtaccc cgctgacggc ggcctggaag
     4861 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     4921 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     4981 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     5041 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatggagggg
     5101 gcagcggcgg ggaaggaagg ggctccctgc tcacctgcgg cgacgtggaa gagaaccccg
     5161 gacctgtcga catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     5221 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     5281 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     5341 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5401 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5461 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5521 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggcgga gggggcagcg
     5581 gcggggaagg aaggggctcc ctgctcacct gcggcgacgt ggaagagaac cccggaccta
     5641 tgcattgatg ataatctaga ggatccgcta gcggccgctc aacctctgga ttacaaaatt
     5701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6061 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6241 atctcccttt gggccgcctc cccgcaggta cctttaagac caatgactta caaggcagct
     6301 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6361 agaagtcaag atctgctttt tgcctgtact gggtctctct ggttagacca gagtctctct
     6421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtttaaacc
     6601 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6661 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6721 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6781 agcaaggggg aggattggga agtcaatagc aggcatgctg gggatgcggt gggctctatg
     6841 ggcggccgtt aattaagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6961 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7081 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7141 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7981 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8041 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     9061 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     9121 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     9181 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     9241 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     9301 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     9361 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     9421 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     9481 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     9541 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9601 gtgctgcaag gcgattaagt tggg
//
