LOCUS       sequence-23317-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404GALS
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     411..434
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     455..471
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     455..471
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(505..521)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(506..522)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      524..771
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(540..558)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(789..808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(790..808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(818..835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(818..834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattaaccct cactaaaggg aacaaagctg gagctcggaa gactctcctc cgtgcgtcct
       61 cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca
      121 ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg
      181 gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta
      241 gttttttagc cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa
      301 cagatatata aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt
      361 ttgtattact tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc
      421 tatactttaa cgtcaaggag aaaaaacccc ggattctaga actagtggat cccccgggct
      481 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagtcatgta attagttatg
      541 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
      601 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
      661 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
      721 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac
      781 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaac
//
