LOCUS       sequence-23318-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p406GALL
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     408..431
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     452..468
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     452..468
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(502..518)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(503..519)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(537..555)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gaacaaagcg ggagctcgtg acagccctcc gaaggaagac tctcctccgt gcgtcctcgt
       61 cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata
      121 aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag taacctggcc
      181 ccacaaacct tcaaatgaac gaatcaaatt aacaaccata ggatgataat gcgattagtt
      241 ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat ctattaacgg
      301 atatataaat gcaaaaactg cataaccact ttaactaata ctttcaacat tttcggtttg
      361 tattacttct tattcaaatg taataaaagt atcaacaaaa aattgttaat atacctctat
      421 actttaacgt caaggagaaa aaaccccgga ttctagaact agtggatccc ccgggctgca
      481 ggaattcgat atcaagctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca
      541 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
      601 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
      661 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
      721 aaaaccttgc t
//
