LOCUS       sequence-233192-       16282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BacMam vector for Ptch1-B NNQ variant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers 
            BR, Cho W, Cheng Y, Beachy PA
  TITLE     Structural Basis for Cholesterol Transport-like Activity of the 
            Hedgehog Receptor Patched.
  JOURNAL   Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 
            10.1016/j.cell.2018.10.026. Epub 2018 Nov 8.
  PUBMED    30415841
REFERENCE   2  (bases 1 to 16282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10.026"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1352-1364.e14"
COMMENT     Sequence Label: pVLAD6-mmPtch1-B-NNQ-TEV-SBP
FEATURES             Location/Qualifiers
     source          1..16282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..21)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4..20)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..35)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(229..248)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     348..370
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(408..426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2506..2527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2542..2547,2548..2565,2566..2572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2580..2596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2585..2607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2604..2620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2604..2620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3837..3854
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     4288..4993
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             4288..4876
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     3'UTR           4533..4905
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        5616..5995
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5996..6199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6150..6170
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6196..6220
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          6335..6467
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6450..6469
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        6512..6530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10370..10390
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             10394..10507
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(10520..10522,10523..11236)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10562..10583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10823..10842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11170..11191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             11240..11263
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             11273..11308
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             11309..11332
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    11336..11924
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11389..11409)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11807..11818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11936..12057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11947..11966)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12001..12020
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(12191..12282)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(12249..12266)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     12286..13113
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             12346..12951
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(12989..13113)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
ORIGIN
        1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
       61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattggccgc
     2641 cggttagcaa cttgtatgtt aacacgccga cggcgtacca gtcaaacgaa cgtgcatagt
     2701 tgtggtgtcg aattttttcc ggactaaaat actccaacgt gccgtcgtgc acgctaagtg
     2761 agttttcgtg tttgcacaat ccgtaatcgc aaacatacac gcgatcaagt gcttcgaaat
     2821 ataagacatt ttcgagtttt atgtcgttgt gtatgaaatt gtgcttgtgc aaatcgttga
     2881 gcgcttcaca cagctgtcta ataatattgc taacaagttg gtacgaaagc tcgcctttaa
     2941 tttgtagtgt ttcaaataaa tcgggacagt caatgtagtc catcacgatc acttggttgt
     3001 tgatggaacc gtgattaaaa tagattttta taaaatttgg gtggtcgctc attaactggt
     3061 gaacttttat ttcgtcagcg ttaaaattgt gcgccgaaat tgttttctgc aaatacaatt
     3121 tgctagtggg cttgtgcgat aaaatagata ttttgccaaa gcgcccgttg attattttat
     3181 accgagtttt gacgtttttg cagttttcgt aaaattgtac cagcgtttgc agcgtagcat
     3241 ttgtggtggc catgacgaat cgtagatatg aatctgtaca atcttattta ttcaataata
     3301 ggaacaacaa aattgatgcg catcaatttt ttgaacgtgt cgatactgcg gaagcgcaaa
     3361 ttatcaaaaa caacatttac gacaacacag tggtgctgaa cagagatgtt cttttaaata
     3421 ttttaaagct tgccaacgac gtttttgaca acaaagcata catgtacgtc gacgacagcg
     3481 aagtgtctcg ccactacaat gctgtagtaa aaatgaaaag gcttgttatc ggcgtgcgag
     3541 cttgagacaa tctctttaca atacaattgc ttacattgag cggttgttaa atatcggcac
     3601 ggtaaacgat agcgaaatta caatgcttat agcagatttt tacgatttgt attccaatta
     3661 taatattgaa ttgccaccac cccaagcgct gcctcgaagc agacgacctt ccgttgtgca
     3721 gccagcggcg cctgcgccgg tgcccacaat cgtgcgcgaa caaactaaac cagaacaaat
     3781 tataccggcg gcaccgccgc caccaccttc tcccgtgcct aacattccag cgcctccacc
     3841 accaccacca ccatcgatgt ctgaattgcc gcccgctcca ccaatgccga cggaacctca
     3901 acccgctgca cctttagacg acagacaaca attgttggaa gctattagaa acgaaaaaaa
     3961 tcgcactcgt ctcagaccgg tcaaaccaaa aacggcgccc gaaaccagta caatagttga
     4021 ggtgccgact gtgttgccta aagagacatt tgagcctaaa ccgccgtctg catcaccgcc
     4081 accacctccg cctccgcctc cgccgccagc cccgcctgcg cctccaccga tggtagattt
     4141 atcatcagct ccaccaccgc cgccattagt agatttgccg tctgaaatgt taccaccgcc
     4201 tgcaccatcg ctttctaacg tgttgtctga attaaaatcg ggcacagtta gattgaaacc
     4261 cgcccaaaaa cgcccgcaat cagaaataat tccaaaaagc tcaactacaa atttgatcgc
     4321 ggacgtgtta gccgacacaa ttaataggcg tcgtgtggct atggcaaaat cgtcttcgga
     4381 agcaacttct aacgacgagg gttgggacga cgacgataat cggcctaata aagctaacac
     4441 gcccgatgtt aaatatgtcc aagctttatt taacgtgttt acgtcgagtc aattgtacac
     4501 taacgacagt gatgaaagaa atacaaaagc gcataatatt ttgaacgacg tcgaaccttt
     4561 attacaaaac aaaacacaaa cgaatatcga caaagctaga ttgctgctac aagatttggc
     4621 aagttttgtg gcgttgagcg aaaatccatt agatagtcca gccatcggtt cggaaaaaca
     4681 acccttgttt gaaactaatc gaaacctatt ttacaaatct attgaggatt taatatttaa
     4741 attcagatat aaagacgctg aaaatcattt gattttcgct ctaacatacc accctaaaga
     4801 ttataaattt aatgaattat taaaatacgt acaacaattg tctgtaaatc aacaacgcac
     4861 agaatctagc gcttaataaa tgtactaata acaatgtatc gtgttttaat acgccggacc
     4921 agtgaacaga ggtgcgtctg gtgcaaactc ctttactttg aacaccaggg aaacttcaag
     4981 gagaatttcc tcctcttcag cagagtcggt accgatgtaa acgatgggct tgtagaagtt
     5041 ctcccagatg acacgatcga tgaactgttc gaacgagttg gtgtactcag agtgaaggtt
     5101 cattattggg cagccgccgc ccttcttagc caggctgatg cggtactcgt tgttgctgcc
     5161 cacccatgaa ggctcgacga tcctaatcac gtcatgaggt acatagtcgg ggtcgcaacg
     5221 cagagcgtgt tgggccagga atttgtaaca acggttgggt ctagtgggac gcatgttgac
     5281 aacaaggaaa acatccatca cttcttggtc gttaacaatg gggaagctgt cttccatgaa
     5341 gcgggtccaa gtttccctgt agaactcttt tcctttccat ccaacgacaa gcttcatcgt
     5401 gtcgggttta acattacgga tttccttgaa gagagtgagt ttttggttct tgccgggtcc
     5461 caggaaagga tcagatcttc aatattggcc attagccata ttattcattg gttatatagc
     5521 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     5581 ttatattggc tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta
     5641 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5701 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5761 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5821 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
     5881 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5941 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6001 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6061 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6121 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga
     6181 ggtctatata agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt
     6241 agtttatcac agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt
     6301 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
     6361 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
     6421 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
     6481 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga
     6541 gaattcacgc gtggtacctc tagacaccat ggcctcggct ggtaacgccg ccggggccct
     6601 gggcaggcag gccggcggcg ggaggcgcag acggaccggg ggaccgcacc gcgccgcgcc
     6661 ggaccgggac tatctgcacc ggcccagcta ctgcgacgcc gccttcgctc tggagcagat
     6721 ttccaagggg aaggctactg gccggaaagc gccgctgtgg ctgagagcga agtttcagag
     6781 actcttattt aaactgggtt gttacattca aaagaactgc ggcaagtttt tggttgtggg
     6841 tctcctcata tttggggcct tcgctgtggg attaaaggca gctaatctcg agaccaacgt
     6901 ggaggagctg tgggtggaag ttggtggacg agtgagtcga gaattaaatt atacccgtca
     6961 gaagatagga gaagaggcta tgtttaatcc tcaactcatg atacagactc caaaagaaga
     7021 aggcgctaat gttctgacca cagaggctct cctgcaacac ctggactcag cactccaggc
     7081 cagtcgtgtg cacgtctaca tgtataacag gcaatggaag ttggaacatt tgtgctacaa
     7141 atcaggggaa cttatcacgg agacaggtta catggatcag ataatagaat acctttaccc
     7201 ttgcttaatc attacacctt tggactgctt ctgggaaggg gcaaagctac agtccgggac
     7261 agcatacctc ctaggtaagc ctcctttacg gtggacaaac tttgacccct tggaattcct
     7321 agaagagtta aagaaaataa actaccaagt ggacagctgg gaggaaatgc tgaataaagc
     7381 cgaagttggc catgggtaca tggaccggcc ttgcctcaac ccagccgacc cagattgccc
     7441 tgccacagcc cctaacaaaa attcaaccaa acctcttgat gtggcccttg ttttgaatgg
     7501 tggatgtcaa ggtttatcca ggaagtatat gcattggcag gaggagttga ttgtgggtgg
     7561 taccgtcaag aatgccactg gaaaacttgt cagcgctcac gccctgcaaa ccatgttcca
     7621 gttaatgact cccaagcaaa tgtatgaaca cttcaggggc tacgactatg tctctcacat
     7681 caactggaat gaagacaggg cagccgccat cctggaggcc tggcagagga cttacgtgga
     7741 ggtggttcat caaagtgtcg ccccaaactc cactcaaaag gtgcttccct tcacaaccac
     7801 gaccctggac gacatcctaa aatccttctc tgatgtcagt gtcatccgag tggccagcgg
     7861 ctacctactg atgcttgcct atgcctgttt aaccatgctg cgctgggact gctccaagtc
     7921 ccagggtgcc gtggggctgg ctggcgtcct gttggttgcg ctgtcagtgg ctgcaggatt
     7981 gggcctctgc tccttgattg gcatttcttt taatgctgcg acaactcagg ttttgccgtt
     8041 tcttgctctt ggtgttggtg tgaataatgt cttcctcctg gcccatgcat tcagtgaaac
     8101 aggacagaat aagaggattc catttgagga caggactggg gagtgcctca agcgcaccgg
     8161 agccagcgtg gccctcacct ccatcagcaa tgtcaccgcc ttcttcatgg ccgcattgat
     8221 ccctatccct gccctgcgag cgttctccct ccaggctgct gtggtggtgg tattcaattt
     8281 tgctatggtt ctgctcattt ttcctgcaat tctcagcatg gatttataca gacgtgagga
     8341 cagaagattg gatattttct gctgtttcac aagcccctgt gtcagcaggg tgattcaagt
     8401 tgagccacag gcctacacag agcctatgca gtccaccgtt cagctccgca cagagtatga
     8461 ccctcacacg cacgtgtact acaccaccgc cgagccacgc tctgagatct ctgtacagcc
     8521 tgttaccgtc acccaggaca acctcagctg tcagagtccc gagagcacca gctctaccag
     8581 ggacctgctc tcccagttct cagactccag cctccactgc ctcgagcccc cctgcaccaa
     8641 gtggacactc tcttcgtttg cagagaagca ctatgctcct ttcctcctga aacccaaagc
     8701 caaggttgtg gtaatccttc ttttcctggg cttgctgggg gtcagccttt atgggaccac
     8761 ccgagtgaga gacgggctgg acctcacgga cattgttccc cgggaaacca gagaatatga
     8821 cttcatagct gcccagttca agtacttctc tttctacaac atgtatatag tcacccagaa
     8881 agcagactac ccgaatatcc agcacctact ttacgacctt cataagagtt tcagcaatgt
     8941 gaagtatgtc atgctggagg agaacaagca acttccccaa atgtggctgc actactttag
     9001 agactggctt caaggacttc aggatgcatt tgacagtgac tgggaaactg ggaggatcat
     9061 gccaaacaat tataaaaatg gatcagatga cggggtcctc gcttacaaac tcctggtgca
     9121 gactggcagc cgagacaagc ccatcgacat tagtcagttg actaaacagc gtctggtaga
     9181 cgcagatggc atcattaatc cgagcgcttt ctacatctac ctgaccgctt gggtcagcaa
     9241 cgaccctgta gcttacgctg cctcccaggc caacatccgg cctcaccggc cggagtgggt
     9301 ccatgacaaa gccgactaca tgccagagac caggctgaga atcccagcag cagagcccat
     9361 cgagtacgct cagttccctt tctacctcaa cggcctacga gacacctcag actttgtgga
     9421 agccatagaa aaagtgagag tcatctgtaa caactatacg agcctgggac tgtccagcta
     9481 ccccaatggc taccccttcc tgttctggga gcaatacatc agcctgcgcc actggctgct
     9541 gctatccatc agcgtggtgc tggcctgcac gtttctagtg tgcgcagtct tcctcctgaa
     9601 cccctggacg gccgggatca ttgtcatggt cctggctctg atgaccgttg agctctttgg
     9661 catgatgggc ctcattggga tcaagctgag tgctgtgcct gtggtcatcc tgattgcatc
     9721 tgttggcatc ggagtgcagt tcaccgtcca cgtggctttg gcctttctga cagccattgg
     9781 ggacaagaac cacagggcta tgctcgctct ggaacacatg tttgctcccg ttctggacgg
     9841 tgctgtgtcc actctgctgg gtgtactgat gcttgcaggg tccgaatttg atttcattgt
     9901 cagatacttc tttgccgtcc tggccattct caccgtcttg ggggttctca atggactggt
     9961 tctgctgcct gtcctcttat ccttctttgg accgtgtcct gaggtgtctc cagccaatgg
    10021 cctaaaccga ctgcccactc cttcgcctga gccgcctcca agtgtcgtcc ggtttgccgt
    10081 gcctcctggt cacacgaaca atgggtctga ttcctccgac tcggagtaca gctctcagac
    10141 cacggtgtct ggcatcagtg aggagctcag gcaatacgaa gcacagcagg gtgccggagg
    10201 ccctgcccac caagtgattg tggaagccac agaaaaccct gtctttgccc ggtccactgt
    10261 ggtccatccg gactccagac atcagcctcc cttgacccct cggcaacagc cccacctgga
    10321 ctctggctcc ttgtcccctg gacggcaagg ccagcagcct cgaagggatg aaaatctgta
    10381 cttccagtcc ggcatggatg aaaagaccac cggttggcgt ggtggtcatg ttgttgaagg
    10441 tctggcaggc gaactggaac agctgcgtgc acgtctggaa catcatccgc agggtcagcg
    10501 tgaaccgtaa gcggccgcag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
    10561 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
    10621 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
    10681 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
    10741 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
    10801 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
    10861 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
    10921 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
    10981 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
    11041 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
    11101 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
    11161 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
    11221 cgagctgtac aagtaggcat tggaagttct gtttcaaggg cctggggccg cagaagatca
    11281 agttgatcca agattgattg atggcaagca tcaccatcac catcaccatc actagaatca
    11341 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    11401 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    11461 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    11521 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    11581 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    11641 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    11701 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
    11761 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    11821 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    11881 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga catgataaga
    11941 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
    12001 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
    12061 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
    12121 agcaagtaaa acctctacaa atgtggtagg atccgcgccc gatggtggga cggtatgaat
    12181 aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt
    12241 atttatttgc gagatggtta tcattttaat tatctccatg atatcagcaa ctatatattg
    12301 atagacattt ccagtttgtg atattagttt gtgcgtctca ttacaatggc tgttattttt
    12361 aacaacaaac aactgctcgc agacaatagt atagaaaagg gaggtgaact gtttttgttt
    12421 aacggttcgt acaacatttt ggaaagttat gttaatccgg tgctgctaaa aaatggtgta
    12481 attgaactag aagaagctgc gtactatgcc ggcaacatat tgtacaaaac cgacgatccc
    12541 aaattcattg attatataaa tttaataatt aaagcaacac actccgaaga actaccagaa
    12601 aatagcactg ttgtaaatta cagaaaaact atgcgcagcg gtactataca ccccattaaa
    12661 aaagacatat atatttatga caacaaaaaa tttactctat acgatagata catatatgga
    12721 tacgataata actatgttaa tttttatgag gagaaaaatg aaaaagagaa ggaatacgaa
    12781 gaagaagacg acaaggcgtc tagtttatgt gaaaataaaa ttatattgtc gcaaattaac
    12841 tgtgaatcat ttgaaaatga ttttaaatat tacctcagcg attataacta cgcgttttca
    12901 attatagata atactacaaa tgttcttgtt gcgtttggtt tgtatcgtta ataaaaaaca
    12961 aatttgacat ttataattgt tttattattc aataattaca aataggattg agacccttgc
    13021 agttgccagc aaacggacag agcttgtcga cagttttcat tttttggcag tttggtggtt
    13081 tatacacatc ttgggatttg attaaaagat gcatgacttc acccacacac ttggagtcgt
    13141 ggttgtagaa atgcgtcaga gccttgatta aacatcggtt ttcacacttg gcacacgaaa
    13201 ccacgtttgc aatgtaacag tttagtataa acctcttcct gtacataccg ccgcgcggta
    13261 cattgttttc tttcaaatcg ttcaatattt tttttataca cggaggcata ttaatcttgt
    13321 tgttcagcat gtcgacgaca tttttctttc cttctacgca ttctttcata caaatgtttc
    13381 tcttgctgcg ttttttaaaa ttggaatttt taatttcatt aatagtgggc ggagccgtta
    13441 acagcatgta caatcgcagt ccggaaattt tgaccaaaac cccgccattg tagaataccg
    13501 tgtatggagt tagggtcaat ttatcaataa aatcatcggg atcaattaaa tatgtagctt
    13561 tcttgtctaa acatgacgct ctaatgagcg gactccacac gttatacgat gcattcgcca
    13621 tacttcgcgg cttctcgcac cagcggtcct gattcgacta acggtcgatt ggatggctta
    13681 atagttttaa cgtcaccgac atactcggta attttgtagt cggcagttct tttgggcgtg
    13741 atcgttgtca ctacgggggt catgtcatcg cttcgtaaaa acttattaca atcaaagctc
    13801 gtgccggaac gcgtgcacag atcgtgcacg ttgcgtcgca agctatttaa ttcgcgcttt
    13861 atttgatctc tatcatagtc gtgttgagat gcgacaacgg gcgcattgtt aattcttgac
    13921 gtgcgataca taatatatta ttggataaag ttgcattaaa tgaaactaac ttacaagatg
    13981 gctagtttgt taaaatacgc gctgcgcttg actcgggaat acaaagaaaa cattattcca
    14041 cactttgatc acttgactcg attgcgcgat ttaatcgacg gcatgattaa aagcgaggat
    14101 gtacaacgtt ttaatcgcac taatcgcaat gatttaattt cggcttgcat gcaaatcaac
    14161 gttcggacgt acatgcccaa cgccacgata gatatgcgca aacaacccaa ctgtatatat
    14221 tttcgaattt gccaatattg ccacttggag gccgacgtgc cttcgcccga cgatcattcg
    14281 gtgtacagat acttgtgcgt cgcgtgcggc acgccgctgg tcatcgacca cccgctcgac
    14341 gtgttcggcc acacggagga aggcgtcaac gaactgctcg aggtgcagcg agtcaacgcg
    14401 ggcggggagt tgtaggcgtc ataactattt attaaataag ataatttaaa aaatcgccgt
    14461 taatatgcaa atcaaaactg tactattggc ttttgcgatg tttgccgcgt tgaacgcgca
    14521 acacgtttta gccgcttgcg ccgagaccgg cgccgtctgc gttcataacg acgagtgttg
    14581 tagcggcgca tgttccccca tatttaatta ttgcttacca caataagttt agtttgcgtt
    14641 aaaaaacaat agttgttgca aaatcgacac acgtgtatca aaatggctcg cgttaaaatt
    14701 ggagaattta aatttggaga agatacgttc aacctgcggt acgtgctaga gcgcgaccaa
    14761 caggtccggt tcgtggccaa ggacgtcgct aacagtttaa aatatacagt ttgtgataaa
    14821 gctatacgtg ttcatgtaga caataaatac aaatcgttat ttgagcagac catccaaaat
    14881 gggggcccta cctctaacag cgtcgtgaaa aggggcgacc cgctgtattt gcagccgcat
    14941 acagtgctca ttactaaatc cggcgtgatt cagctgatta tgaagtccaa attgccttac
    15001 gccatagaat tacaagaatg gcttttagaa gaagtaattc ctcaagtgct ctgcaccggc
    15061 aagtacgatc ccgcgatcaa acaacgggag gaggagagca aacagttggt gactaagctg
    15121 attgcgacat tcaccgagca cacaaacgcg ctgcaagcgg tggtggcgca aaaaaccgag
    15181 gaacttgtta aaaaacaaga gtttattgaa cgcatcgttg ccatcaagga caagcagatc
    15241 gaggccaaag atttgcaggt cacgcgcgtc atgactgatc taaaccgcat gtacaccggc
    15301 ttccaggaaa ccatgcaaaa gaaagacgaa ataatgcaga aaaaagacgc gcaggtcacc
    15361 gatttagttg ccaaagtggt ggatttgtcg gatcgcgcgg ttcaataccc cgcggacaag
    15421 cgcaaacatc cggtgttgtg cgtgacgcgc gacggcacta cgtttacggc tattaccggc
    15481 caaaagacgt acgtggaaaa ccaaaagcat aaacgtaaca tcaacgttgc caacattgtg
    15541 gtggagaata tccggcctaa tcctaccgtc gattggaaca acgccactga tcggctacaa
    15601 gctaaacgga gcaagcgaag catagttttg gttcgctgga agaagcgcaa caatttgaaa
    15661 ataggataaa gtatttgtta aagaatgcaa ataatattaa ttaaattaat aaatcttgaa
    15721 cgtaattttg tctttcaatt ttgtgacctc tggctttttc gaatctatct atggcttcct
    15781 gcggcgcaat acccagggtg tgcattaaat atctgcacac catgtaaccg gtgcgattaa
    15841 taccgtgtgt gcagtgcacg cccaccaaca tgccgggaca cttttctgta aattctttta
    15901 ccgtgtcaat aaattcttga actatgcttt caggcggcaa agtctggcca ggtacttgaa
    15961 tttttttgta taacaggccc gcccgcaaaa aatgcacacc atcataatat ttagacgtgt
    16021 tggttaaatc gattattgct ccaatactag ggttttgttt tactatctgt tctgcggtcc
    16081 acatatcttc ttcgctagtc acgtacgcaa acaactcggg ccgcaaaggt gttttaaaac
    16141 atatcagatt agaatctttt ataacttggc cgcattgtaa atagttgtgc caacgcgcgg
    16201 gaaacatttt acacgtgctt tcaatcttat ctcataaacg caactaaata tgatccttca
    16261 actcgcttta cgagccaagc tt
//
