LOCUS       sequence-23322-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.36
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      178..501
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(505..600)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(637..656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(638..656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(661..677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(661..677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(779..798)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(857..876)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctagagctcg aattctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggataaatg
      181 caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat
      241 tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg
      301 aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct gatgtacttt
      361 cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg agaaaaatgc
      421 gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag gaaactttat
      481 tgtgccgcca agaaaaaagt ctcacaactt tattatacaa agttggcatt ataaaaaagc
      541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
      601 ggagctccat ggtagcgtta acgcggccgc gatatcccct atagtgagtc gtattacatg
      661 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
      721 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      781 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
      841 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc angtgcgaca
      901 atctatcgct tgtatgggaa gcccgatgcg cca
//
