LOCUS       sequence-233221-       16258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BacMam vector for Ptch1-B expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers 
            BR, Cho W, Cheng Y, Beachy PA
  TITLE     Structural Basis for Cholesterol Transport-like Activity of the 
            Hedgehog Receptor Patched.
  JOURNAL   Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 
            10.1016/j.cell.2018.10.026. Epub 2018 Nov 8.
  PUBMED    30415841
REFERENCE   2  (bases 1 to 16258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10.026"; journalName: "Cell"; date: 
            "2018-11-15- 15"; volume: "175"; issue: "5"; pages: "1352-1364.e14"
COMMENT     Sequence Label: pVLAD6-mmPtch1-B-SBP
FEATURES             Location/Qualifiers
     source          1..16258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..21)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4..20)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..35)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(229..248)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     348..370
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(408..426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2506..2527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2542..2547,2548..2565,2566..2572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2580..2596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2585..2607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2604..2620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2604..2620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3837..3854
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     4288..4993
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             4288..4876
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     3'UTR           4533..4905
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        5616..5995
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5996..6199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6150..6170
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6196..6220
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          6335..6467
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6450..6469
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        6512..6530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10370..10483
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(10496..10498,10499..11212)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10538..10559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10799..10818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11146..11167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             11216..11239
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             11249..11284
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             11285..11308
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    11312..11900
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11365..11385)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11783..11794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11912..12033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11923..11942)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11977..11996
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(12167..12258)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(12225..12242)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     12262..13089
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             12322..12927
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(12965..13089)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
ORIGIN
        1 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
       61 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      241 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      301 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      361 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      421 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2461 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2521 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2581 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattggccgc
     2641 cggttagcaa cttgtatgtt aacacgccga cggcgtacca gtcaaacgaa cgtgcatagt
     2701 tgtggtgtcg aattttttcc ggactaaaat actccaacgt gccgtcgtgc acgctaagtg
     2761 agttttcgtg tttgcacaat ccgtaatcgc aaacatacac gcgatcaagt gcttcgaaat
     2821 ataagacatt ttcgagtttt atgtcgttgt gtatgaaatt gtgcttgtgc aaatcgttga
     2881 gcgcttcaca cagctgtcta ataatattgc taacaagttg gtacgaaagc tcgcctttaa
     2941 tttgtagtgt ttcaaataaa tcgggacagt caatgtagtc catcacgatc acttggttgt
     3001 tgatggaacc gtgattaaaa tagattttta taaaatttgg gtggtcgctc attaactggt
     3061 gaacttttat ttcgtcagcg ttaaaattgt gcgccgaaat tgttttctgc aaatacaatt
     3121 tgctagtggg cttgtgcgat aaaatagata ttttgccaaa gcgcccgttg attattttat
     3181 accgagtttt gacgtttttg cagttttcgt aaaattgtac cagcgtttgc agcgtagcat
     3241 ttgtggtggc catgacgaat cgtagatatg aatctgtaca atcttattta ttcaataata
     3301 ggaacaacaa aattgatgcg catcaatttt ttgaacgtgt cgatactgcg gaagcgcaaa
     3361 ttatcaaaaa caacatttac gacaacacag tggtgctgaa cagagatgtt cttttaaata
     3421 ttttaaagct tgccaacgac gtttttgaca acaaagcata catgtacgtc gacgacagcg
     3481 aagtgtctcg ccactacaat gctgtagtaa aaatgaaaag gcttgttatc ggcgtgcgag
     3541 cttgagacaa tctctttaca atacaattgc ttacattgag cggttgttaa atatcggcac
     3601 ggtaaacgat agcgaaatta caatgcttat agcagatttt tacgatttgt attccaatta
     3661 taatattgaa ttgccaccac cccaagcgct gcctcgaagc agacgacctt ccgttgtgca
     3721 gccagcggcg cctgcgccgg tgcccacaat cgtgcgcgaa caaactaaac cagaacaaat
     3781 tataccggcg gcaccgccgc caccaccttc tcccgtgcct aacattccag cgcctccacc
     3841 accaccacca ccatcgatgt ctgaattgcc gcccgctcca ccaatgccga cggaacctca
     3901 acccgctgca cctttagacg acagacaaca attgttggaa gctattagaa acgaaaaaaa
     3961 tcgcactcgt ctcagaccgg tcaaaccaaa aacggcgccc gaaaccagta caatagttga
     4021 ggtgccgact gtgttgccta aagagacatt tgagcctaaa ccgccgtctg catcaccgcc
     4081 accacctccg cctccgcctc cgccgccagc cccgcctgcg cctccaccga tggtagattt
     4141 atcatcagct ccaccaccgc cgccattagt agatttgccg tctgaaatgt taccaccgcc
     4201 tgcaccatcg ctttctaacg tgttgtctga attaaaatcg ggcacagtta gattgaaacc
     4261 cgcccaaaaa cgcccgcaat cagaaataat tccaaaaagc tcaactacaa atttgatcgc
     4321 ggacgtgtta gccgacacaa ttaataggcg tcgtgtggct atggcaaaat cgtcttcgga
     4381 agcaacttct aacgacgagg gttgggacga cgacgataat cggcctaata aagctaacac
     4441 gcccgatgtt aaatatgtcc aagctttatt taacgtgttt acgtcgagtc aattgtacac
     4501 taacgacagt gatgaaagaa atacaaaagc gcataatatt ttgaacgacg tcgaaccttt
     4561 attacaaaac aaaacacaaa cgaatatcga caaagctaga ttgctgctac aagatttggc
     4621 aagttttgtg gcgttgagcg aaaatccatt agatagtcca gccatcggtt cggaaaaaca
     4681 acccttgttt gaaactaatc gaaacctatt ttacaaatct attgaggatt taatatttaa
     4741 attcagatat aaagacgctg aaaatcattt gattttcgct ctaacatacc accctaaaga
     4801 ttataaattt aatgaattat taaaatacgt acaacaattg tctgtaaatc aacaacgcac
     4861 agaatctagc gcttaataaa tgtactaata acaatgtatc gtgttttaat acgccggacc
     4921 agtgaacaga ggtgcgtctg gtgcaaactc ctttactttg aacaccaggg aaacttcaag
     4981 gagaatttcc tcctcttcag cagagtcggt accgatgtaa acgatgggct tgtagaagtt
     5041 ctcccagatg acacgatcga tgaactgttc gaacgagttg gtgtactcag agtgaaggtt
     5101 cattattggg cagccgccgc ccttcttagc caggctgatg cggtactcgt tgttgctgcc
     5161 cacccatgaa ggctcgacga tcctaatcac gtcatgaggt acatagtcgg ggtcgcaacg
     5221 cagagcgtgt tgggccagga atttgtaaca acggttgggt ctagtgggac gcatgttgac
     5281 aacaaggaaa acatccatca cttcttggtc gttaacaatg gggaagctgt cttccatgaa
     5341 gcgggtccaa gtttccctgt agaactcttt tcctttccat ccaacgacaa gcttcatcgt
     5401 gtcgggttta acattacgga tttccttgaa gagagtgagt ttttggttct tgccgggtcc
     5461 caggaaagga tcagatcttc aatattggcc attagccata ttattcattg gttatatagc
     5521 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     5581 ttatattggc tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta
     5641 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5701 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     5761 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     5821 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
     5881 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     5941 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6001 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6061 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6121 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga
     6181 ggtctatata agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt
     6241 agtttatcac agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt
     6301 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
     6361 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
     6421 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
     6481 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga
     6541 gaattcacgc gtggtacctc tagacaccat ggcctcggct ggtaacgccg ccggggccct
     6601 gggcaggcag gccggcggcg ggaggcgcag acggaccggg ggaccgcacc gcgccgcgcc
     6661 ggaccgggac tatctgcacc ggcccagcta ctgcgacgcc gccttcgctc tggagcagat
     6721 ttccaagggg aaggctactg gccggaaagc gccgctgtgg ctgagagcga agtttcagag
     6781 actcttattt aaactgggtt gttacattca aaagaactgc ggcaagtttt tggttgtggg
     6841 tctcctcata tttggggcct tcgctgtggg attaaaggca gctaatctcg agaccaacgt
     6901 ggaggagctg tgggtggaag ttggtggacg agtgagtcga gaattaaatt atacccgtca
     6961 gaagatagga gaagaggcta tgtttaatcc tcaactcatg atacagactc caaaagaaga
     7021 aggcgctaat gttctgacca cagaggctct cctgcaacac ctggactcag cactccaggc
     7081 cagtcgtgtg cacgtctaca tgtataacag gcaatggaag ttggaacatt tgtgctacaa
     7141 atcaggggaa cttatcacgg agacaggtta catggatcag ataatagaat acctttaccc
     7201 ttgcttaatc attacacctt tggactgctt ctgggaaggg gcaaagctac agtccgggac
     7261 agcatacctc ctaggtaagc ctcctttacg gtggacaaac tttgacccct tggaattcct
     7321 agaagagtta aagaaaataa actaccaagt ggacagctgg gaggaaatgc tgaataaagc
     7381 cgaagttggc catgggtaca tggaccggcc ttgcctcaac ccagccgacc cagattgccc
     7441 tgccacagcc cctaacaaaa attcaaccaa acctcttgat gtggcccttg ttttgaatgg
     7501 tggatgtcaa ggtttatcca ggaagtatat gcattggcag gaggagttga ttgtgggtgg
     7561 taccgtcaag aatgccactg gaaaacttgt cagcgctcac gccctgcaaa ccatgttcca
     7621 gttaatgact cccaagcaaa tgtatgaaca cttcaggggc tacgactatg tctctcacat
     7681 caactggaat gaagacaggg cagccgccat cctggaggcc tggcagagga cttacgtgga
     7741 ggtggttcat caaagtgtcg ccccaaactc cactcaaaag gtgcttccct tcacaaccac
     7801 gaccctggac gacatcctaa aatccttctc tgatgtcagt gtcatccgag tggccagcgg
     7861 ctacctactg atgcttgcct atgcctgttt aaccatgctg cgctgggact gctccaagtc
     7921 ccagggtgcc gtggggctgg ctggcgtcct gttggttgcg ctgtcagtgg ctgcaggatt
     7981 gggcctctgc tccttgattg gcatttcttt taatgctgcg acaactcagg ttttgccgtt
     8041 tcttgctctt ggtgttggtg tggatgatgt cttcctcctg gcccatgcat tcagtgaaac
     8101 aggacagaat aagaggattc catttgagga caggactggg gagtgcctca agcgcaccgg
     8161 agccagcgtg gccctcacct ccatcagcaa tgtcaccgcc ttcttcatgg ccgcattgat
     8221 ccctatccct gccctgcgag cgttctccct ccaggctgct gtggtggtgg tattcaattt
     8281 tgctatggtt ctgctcattt ttcctgcaat tctcagcatg gatttataca gacgtgagga
     8341 cagaagattg gatattttct gctgtttcac aagcccctgt gtcagcaggg tgattcaagt
     8401 tgagccacag gcctacacag agcctatgca gtccaccgtt cagctccgca cagagtatga
     8461 ccctcacacg cacgtgtact acaccaccgc cgagccacgc tctgagatct ctgtacagcc
     8521 tgttaccgtc acccaggaca acctcagctg tcagagtccc gagagcacca gctctaccag
     8581 ggacctgctc tcccagttct cagactccag cctccactgc ctcgagcccc cctgcaccaa
     8641 gtggacactc tcttcgtttg cagagaagca ctatgctcct ttcctcctga aacccaaagc
     8701 caaggttgtg gtaatccttc ttttcctggg cttgctgggg gtcagccttt atgggaccac
     8761 ccgagtgaga gacgggctgg acctcacgga cattgttccc cgggaaacca gagaatatga
     8821 cttcatagct gcccagttca agtacttctc tttctacaac atgtatatag tcacccagaa
     8881 agcagactac ccgaatatcc agcacctact ttacgacctt cataagagtt tcagcaatgt
     8941 gaagtatgtc atgctggagg agaacaagca acttccccaa atgtggctgc actactttag
     9001 agactggctt caaggacttc aggatgcatt tgacagtgac tgggaaactg ggaggatcat
     9061 gccaaacaat tataaaaatg gatcagatga cggggtcctc gcttacaaac tcctggtgca
     9121 gactggcagc cgagacaagc ccatcgacat tagtcagttg actaaacagc gtctggtaga
     9181 cgcagatggc atcattaatc cgagcgcttt ctacatctac ctgaccgctt gggtcagcaa
     9241 cgaccctgta gcttacgctg cctcccaggc caacatccgg cctcaccggc cggagtgggt
     9301 ccatgacaaa gccgactaca tgccagagac caggctgaga atcccagcag cagagcccat
     9361 cgagtacgct cagttccctt tctacctcaa cggcctacga gacacctcag actttgtgga
     9421 agccatagaa aaagtgagag tcatctgtaa caactatacg agcctgggac tgtccagcta
     9481 ccccaatggc taccccttcc tgttctggga gcaatacatc agcctgcgcc actggctgct
     9541 gctatccatc agcgtggtgc tggcctgcac gtttctagtg tgcgcagtct tcctcctgaa
     9601 cccctggacg gccgggatca ttgtcatggt cctggctctg atgaccgttg agctctttgg
     9661 catgatgggc ctcattggga tcaagctgag tgctgtgcct gtggtcatcc tgattgcatc
     9721 tgttggcatc ggagtggagt tcaccgtcca cgtggctttg gcctttctga cagccattgg
     9781 ggacaagaac cacagggcta tgctcgctct ggaacacatg tttgctcccg ttctggacgg
     9841 tgctgtgtcc actctgctgg gtgtactgat gcttgcaggg tccgaatttg atttcattgt
     9901 cagatacttc tttgccgtcc tggccattct caccgtcttg ggggttctca atggactggt
     9961 tctgctgcct gtcctcttat ccttctttgg accgtgtcct gaggtgtctc cagccaatgg
    10021 cctaaaccga ctgcccactc cttcgcctga gccgcctcca agtgtcgtcc ggtttgccgt
    10081 gcctcctggt cacacgaaca atgggtctga ttcctccgac tcggagtaca gctctcagac
    10141 cacggtgtct ggcatcagtg aggagctcag gcaatacgaa gcacagcagg gtgccggagg
    10201 ccctgcccac caagtgattg tggaagccac agaaaaccct gtctttgccc ggtccactgt
    10261 ggtccatccg gactccagac atcagcctcc cttgacccct cggcaacagc cccacctgga
    10321 ctctggctcc ttgtcccctg gacggcaagg ccagcagcct cgaagggata tggatgaaaa
    10381 gaccaccggt tggcgtggtg gtcatgttgt tgaaggtctg gcaggcgaac tggaacagct
    10441 gcgtgcacgt ctggaacatc atccgcaggg tcagcgtgaa ccgtaagcgg ccgcagtgag
    10501 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
    10561 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
    10621 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
    10681 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
    10741 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
    10801 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
    10861 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
    10921 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
    10981 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
    11041 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
    11101 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
    11161 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aggcattgga
    11221 agttctgttt caagggcctg gggccgcaga agatcaagtt gatccaagat tgattgatgg
    11281 caagcatcac catcaccatc accatcacta gaatcaacct ctggattaca aaatttgtga
    11341 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    11401 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    11461 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    11521 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    11581 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    11641 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    11701 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11761 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11821 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    11881 cctttgggcc gcctccccgc ctgagacatg ataagataca ttgatgagtt tggacaaacc
    11941 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
    12001 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
    12061 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
    12121 ggtaggatcc gcgcccgatg gtgggacggt atgaataatc cggaatattt ataggttttt
    12181 ttattacaaa actgttacga aaacagtaaa atacttattt atttgcgaga tggttatcat
    12241 tttaattatc tccatgatat cagcaactat atattgatag acatttccag tttgtgatat
    12301 tagtttgtgc gtctcattac aatggctgtt atttttaaca acaaacaact gctcgcagac
    12361 aatagtatag aaaagggagg tgaactgttt ttgtttaacg gttcgtacaa cattttggaa
    12421 agttatgtta atccggtgct gctaaaaaat ggtgtaattg aactagaaga agctgcgtac
    12481 tatgccggca acatattgta caaaaccgac gatcccaaat tcattgatta tataaattta
    12541 ataattaaag caacacactc cgaagaacta ccagaaaata gcactgttgt aaattacaga
    12601 aaaactatgc gcagcggtac tatacacccc attaaaaaag acatatatat ttatgacaac
    12661 aaaaaattta ctctatacga tagatacata tatggatacg ataataacta tgttaatttt
    12721 tatgaggaga aaaatgaaaa agagaaggaa tacgaagaag aagacgacaa ggcgtctagt
    12781 ttatgtgaaa ataaaattat attgtcgcaa attaactgtg aatcatttga aaatgatttt
    12841 aaatattacc tcagcgatta taactacgcg ttttcaatta tagataatac tacaaatgtt
    12901 cttgttgcgt ttggtttgta tcgttaataa aaaacaaatt tgacatttat aattgtttta
    12961 ttattcaata attacaaata ggattgagac ccttgcagtt gccagcaaac ggacagagct
    13021 tgtcgacagt tttcattttt tggcagtttg gtggtttata cacatcttgg gatttgatta
    13081 aaagatgcat gacttcaccc acacacttgg agtcgtggtt gtagaaatgc gtcagagcct
    13141 tgattaaaca tcggttttca cacttggcac acgaaaccac gtttgcaatg taacagttta
    13201 gtataaacct cttcctgtac ataccgccgc gcggtacatt gttttctttc aaatcgttca
    13261 atattttttt tatacacgga ggcatattaa tcttgttgtt cagcatgtcg acgacatttt
    13321 tctttccttc tacgcattct ttcatacaaa tgtttctctt gctgcgtttt ttaaaattgg
    13381 aatttttaat ttcattaata gtgggcggag ccgttaacag catgtacaat cgcagtccgg
    13441 aaattttgac caaaaccccg ccattgtaga ataccgtgta tggagttagg gtcaatttat
    13501 caataaaatc atcgggatca attaaatatg tagctttctt gtctaaacat gacgctctaa
    13561 tgagcggact ccacacgtta tacgatgcat tcgccatact tcgcggcttc tcgcaccagc
    13621 ggtcctgatt cgactaacgg tcgattggat ggcttaatag ttttaacgtc accgacatac
    13681 tcggtaattt tgtagtcggc agttcttttg ggcgtgatcg ttgtcactac gggggtcatg
    13741 tcatcgcttc gtaaaaactt attacaatca aagctcgtgc cggaacgcgt gcacagatcg
    13801 tgcacgttgc gtcgcaagct atttaattcg cgctttattt gatctctatc atagtcgtgt
    13861 tgagatgcga caacgggcgc attgttaatt cttgacgtgc gatacataat atattattgg
    13921 ataaagttgc attaaatgaa actaacttac aagatggcta gtttgttaaa atacgcgctg
    13981 cgcttgactc gggaatacaa agaaaacatt attccacact ttgatcactt gactcgattg
    14041 cgcgatttaa tcgacggcat gattaaaagc gaggatgtac aacgttttaa tcgcactaat
    14101 cgcaatgatt taatttcggc ttgcatgcaa atcaacgttc ggacgtacat gcccaacgcc
    14161 acgatagata tgcgcaaaca acccaactgt atatattttc gaatttgcca atattgccac
    14221 ttggaggccg acgtgccttc gcccgacgat cattcggtgt acagatactt gtgcgtcgcg
    14281 tgcggcacgc cgctggtcat cgaccacccg ctcgacgtgt tcggccacac ggaggaaggc
    14341 gtcaacgaac tgctcgaggt gcagcgagtc aacgcgggcg gggagttgta ggcgtcataa
    14401 ctatttatta aataagataa tttaaaaaat cgccgttaat atgcaaatca aaactgtact
    14461 attggctttt gcgatgtttg ccgcgttgaa cgcgcaacac gttttagccg cttgcgccga
    14521 gaccggcgcc gtctgcgttc ataacgacga gtgttgtagc ggcgcatgtt cccccatatt
    14581 taattattgc ttaccacaat aagtttagtt tgcgttaaaa aacaatagtt gttgcaaaat
    14641 cgacacacgt gtatcaaaat ggctcgcgtt aaaattggag aatttaaatt tggagaagat
    14701 acgttcaacc tgcggtacgt gctagagcgc gaccaacagg tccggttcgt ggccaaggac
    14761 gtcgctaaca gtttaaaata tacagtttgt gataaagcta tacgtgttca tgtagacaat
    14821 aaatacaaat cgttatttga gcagaccatc caaaatgggg gccctacctc taacagcgtc
    14881 gtgaaaaggg gcgacccgct gtatttgcag ccgcatacag tgctcattac taaatccggc
    14941 gtgattcagc tgattatgaa gtccaaattg ccttacgcca tagaattaca agaatggctt
    15001 ttagaagaag taattcctca agtgctctgc accggcaagt acgatcccgc gatcaaacaa
    15061 cgggaggagg agagcaaaca gttggtgact aagctgattg cgacattcac cgagcacaca
    15121 aacgcgctgc aagcggtggt ggcgcaaaaa accgaggaac ttgttaaaaa acaagagttt
    15181 attgaacgca tcgttgccat caaggacaag cagatcgagg ccaaagattt gcaggtcacg
    15241 cgcgtcatga ctgatctaaa ccgcatgtac accggcttcc aggaaaccat gcaaaagaaa
    15301 gacgaaataa tgcagaaaaa agacgcgcag gtcaccgatt tagttgccaa agtggtggat
    15361 ttgtcggatc gcgcggttca ataccccgcg gacaagcgca aacatccggt gttgtgcgtg
    15421 acgcgcgacg gcactacgtt tacggctatt accggccaaa agacgtacgt ggaaaaccaa
    15481 aagcataaac gtaacatcaa cgttgccaac attgtggtgg agaatatccg gcctaatcct
    15541 accgtcgatt ggaacaacgc cactgatcgg ctacaagcta aacggagcaa gcgaagcata
    15601 gttttggttc gctggaagaa gcgcaacaat ttgaaaatag gataaagtat ttgttaaaga
    15661 atgcaaataa tattaattaa attaataaat cttgaacgta attttgtctt tcaattttgt
    15721 gacctctggc tttttcgaat ctatctatgg cttcctgcgg cgcaataccc agggtgtgca
    15781 ttaaatatct gcacaccatg taaccggtgc gattaatacc gtgtgtgcag tgcacgccca
    15841 ccaacatgcc gggacacttt tctgtaaatt cttttaccgt gtcaataaat tcttgaacta
    15901 tgctttcagg cggcaaagtc tggccaggta cttgaatttt tttgtataac aggcccgccc
    15961 gcaaaaaatg cacaccatca taatatttag acgtgttggt taaatcgatt attgctccaa
    16021 tactagggtt ttgttttact atctgttctg cggtccacat atcttcttcg ctagtcacgt
    16081 acgcaaacaa ctcgggccgc aaaggtgttt taaaacatat cagattagaa tcttttataa
    16141 cttggccgca ttgtaaatag ttgtgccaac gcgcgggaaa cattttacac gtgctttcaa
    16201 tcttatctca taaacgcaac taaatatgat ccttcaactc gctttacgag ccaagctt
//
