LOCUS       sequence-23323-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Merritt C, Seydoux G
  TITLE     Transgenic solutions for the germline.
  JOURNAL   WormBook. 2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1.
  PUBMED    20169625
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "WormBook. 
            2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1."
COMMENT     Sequence Label: pCM1.53
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(53..152)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(264..283)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(342..361)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgactataca aaagcccaca agctttggaa ttcgctagcc ggccatacaa gaacccagct
       61 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
      121 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc
      181 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      241 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      301 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
      361 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
      421 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
      481 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
      541 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
      601 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
      661 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
      721 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
      781 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
      841 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
      901 aataggttgt attgatgttg gacga
//
