LOCUS       sequence-233232-       12524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses NSm-GFP and mCherry-HDEL. Used for visualization of 
            cell-to-cell movement of NSm. NSm can be replaced with gene of 
            interest via HiFi DNA assembly cloning (NEB)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Blekemolen, M. C., Tark-Dame, M., Takken, F. L.
  TITLE     Visualization and Quantification of Cell-to-cell Movement of 
            Proteins in Nicotiana benthamiana.
  JOURNAL   Bio-protocol 8(24): e3114.
  PUBMED    29481865
REFERENCE   2  (bases 1 to 12524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Bio-protocol 8(24): e3114."
COMMENT     Sequence Label: NSm-GFP pZK538
FEATURES             Location/Qualifiers
     source          1..12524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             485..1192
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELYK"
     primer_bind     898..917
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1219..1471
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        1929..2273
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3326..3328,3329..3331,3332..4045)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3326..3328,3329..3331,3332..4042)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3371..3392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3632..3651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3979..4000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4304..4321)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4304..4320)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4313..4335)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4523..4547
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5848..6477
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6906..7979
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8045..8239
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8475..8497)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8583..8723
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8638..8657
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8738..8755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8909..9497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8989..9008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9584..10378)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10803..10827
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(10913..11165)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(11175..11726)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(11732..12112)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc agatcactag
      361 tcttaagtcc ggagctagct ctagagagaa gatgaaggta caggagggtt tgttcgtggt
      421 ggctgttttc taccttgctt atacgcagct agtcaagggg caacctcgca aggagtgcgg
      481 atccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      541 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      601 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      661 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      721 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      781 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      841 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
      901 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
      961 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     1021 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     1081 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     1141 cgaacgcgcc gagggccgcc actccaccgg cggcatggag gagctgtaca agagatctca
     1201 tgacgagctg taactgcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     1261 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     1321 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     1381 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     1441 tcgcgcgcgg tgtcatctat gttactagat ccgatgataa gctgtcaaac atgagaattc
     1501 gatatcaagc ttgcatgcct gcaggtcaac atggtggagc acgacacact tgtctactcc
     1561 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
     1621 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
     1681 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
     1741 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1801 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg
     1861 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1921 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1981 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     2041 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     2101 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     2161 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     2221 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacctcgaga
     2281 attcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt
     2341 gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt
     2401 acgaacgata gccatgttga ctttttttgg taataagggg tcttctaagt ctgccagaaa
     2461 ggatgaaggg cctttagttt cacttgctaa acataacggt aatgttgaag tctcaaaacc
     2521 atggtcttct tctgatgaaa agcttgcttt aaccaaagct atggatacat ccaaaggaaa
     2581 gatactgttg aacacagagg gaacatcttc ctttggaaca tatgaatctg attctatcac
     2641 agaatcagag ggttatgatc tttctgcaag aatgatagta gatacaaacc accatatctc
     2701 aaactggaaa aatgatcttt ttgtcggcaa cgggaagcaa aacgctaata aggtcatcaa
     2761 gatctgtcca acttgggaca gcagaaaaca atacatgatg atttccagga ttgtgatatg
     2821 ggtctgcccc actataccaa accctacagg gaaacttgtg gttgctctgg tcgatcccaa
     2881 catgccatct gaaaagcaaa tcattctgaa gggtcagggg acaataactg atcctatctg
     2941 ttttgttttt tatctgaact ggtctattcc gaaaatgaat aacactccag aaaactgctg
     3001 tcagctgcac ttgatgtgca gtcaagaata caagaagggg gtttcttttg gtagtgtcat
     3061 gtattcttgg acaaaggagt tttgtgattc acccagagct gataaagaca aaagttgcat
     3121 ggtcatacct ctaaacaggg ctattagagc tagatctcaa gcattcattg aggcttgcaa
     3181 gctgataatt cctaaaggaa acagtgagaa gcagattaaa aaacagctta aagaactgag
     3241 ctcaaatctt gagagatcag ttgaagaaga ggaggaaggg gtttatgata atgttgctca
     3301 gttatctttt gatgagatag gatccatggt gagcaagggc gaggagctgt tcaccggggt
     3361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3481 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3541 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3781 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3841 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctgtaca agtaagagct cgcgcgcggg gatcctctag agtccgcaaa
     4081 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga
     4141 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     4201 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     4261 cctaaaacca aaatccagtg acctgcaggc atgcaagctt ggcactggcc gtcgttttac
     4321 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4381 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4441 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
     4501 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     4561 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     4621 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     4681 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     4741 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     4801 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     4861 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     4921 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     4981 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     5041 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     5101 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     5161 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     5221 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     5281 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     5341 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     5401 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     5461 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     5521 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     5581 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     5641 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     5701 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     5761 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     5821 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     5881 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     5941 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     6001 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     6061 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     6121 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     6181 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     6241 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     6301 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     6361 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     6421 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     6481 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     6541 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     6601 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     6661 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     6721 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     6781 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     6841 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     6901 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     6961 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     7021 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     7081 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     7141 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     7201 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     7261 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     7321 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     7381 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     7441 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     7501 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     7561 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     7621 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     7681 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     7741 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     7801 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     7861 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     7921 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     7981 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     8041 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     8101 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     8161 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     8221 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     8281 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     8341 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     8401 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     8461 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8521 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8581 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     8641 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8701 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9541 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     9601 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     9661 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9721 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9781 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9841 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9901 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     9961 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
    10021 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    10081 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    10141 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    10201 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    10261 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    10321 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    10381 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    10441 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    10501 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    10561 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10621 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10681 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10741 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    10801 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    10861 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
    10921 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
    10981 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
    11041 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
    11101 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
    11161 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
    11221 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
    11281 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
    11341 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
    11401 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
    11461 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
    11521 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
    11581 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
    11641 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
    11701 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
    11761 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
    11821 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
    11881 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
    11941 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
    12001 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
    12061 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
    12121 attcgagctc cctaccccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg
    12181 gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca
    12241 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
    12301 cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat
    12361 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
    12421 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccacccctac
    12481 tccaaaaatg tcaaagatac agtctcagaa gaccaaaggg ctat
//
