LOCUS       sequence-233234-       10332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Six5 (without signal peptide). Used in combination with 
            Avr2-GFP pZK538(#118011) for visualization of cell-to-cell movement 
            of Avr2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Blekemolen, M. C., Tark-Dame, M., Takken, F. L.
  TITLE     Visualization and Quantification of Cell-to-cell Movement of 
            Proteins in Nicotiana benthamiana.
  JOURNAL   Bio-protocol 8(24): e3114.
  PUBMED    29481865
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Bio-protocol 8(24): e3114."
COMMENT     Sequence Label: Six5 pGWB402
FEATURES             Location/Qualifiers
     source          1..10332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(708..732)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      764..1016
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1049..1065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1049..1065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1062..1084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1073..1089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1097..1103,1104..1121,1122..1127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1142..1163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(1197..1449)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1839..2633)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1950..1969)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2560..2579
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2654..2837)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(2690..2869)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    3043..3067
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3588..4379
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4625..5213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5114..5133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5367..5384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5399..5539
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5465..5484)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5625..5647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5883..6077
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6143..7216)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7645..8274)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9575..9599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9787..9809
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9801..9818
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9802..9818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
      361 gttatcaaca agtttgtaca aaaaagcagg ctggatgagg gatcatcagt actgtgcttg
      421 ccagtccggt tctggtgaca gcattgatat agatgccacc actcagcttc agaacgataa
      481 tagtaaatca tatttgtggg ctcagacctc tccagcatat tggtttgctg accgtcataa
      541 acctggtcca cgattcgctg gcatttatct gaaagctgca aatggcaaaa ttgatggcga
      601 tacattctat aatctttgca ttaataatgg aggagcagac tcaacatgct ttgactgctc
      661 taaatctcac caggttagaa acgttatcta ttgtgatgcg gcctaggacc cagctttctt
      721 gtacaaagtg gttgataaca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
      781 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
      841 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
      901 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
      961 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     1021 attggttccg gaaccaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     1081 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1141 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctga attcccgatc
     1201 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     1261 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     1321 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     1381 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     1441 tgaacgatcg gggatcatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag
     1501 caagatatcg cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg
     1561 gacgccgggc ccgatcatat tgtcgctcag gatcgtggcg ttgtgcttgt cggccgttgc
     1621 tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca gagatcccgt gggcgaagaa
     1681 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     1741 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     1801 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     1861 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     1921 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     1981 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     2041 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcatc gccgtcgggc
     2101 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     2161 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     2221 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     2281 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     2341 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     2401 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     2461 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     2521 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     2581 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     2641 gatccagatc cggtgcagat tatttggatt gagagtgaat atgagactct aattggatac
     2701 cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat
     2761 agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca
     2821 ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca
     2881 aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct
     2941 ccgctcatga tgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa
     3001 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     3061 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     3121 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     3181 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     3241 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     3301 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     3361 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     3421 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     3481 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     3541 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     3601 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     3661 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     3721 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     3781 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     3841 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     3901 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     3961 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     4021 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     4081 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     4141 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     4201 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     4261 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     4321 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     4381 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     4441 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     4501 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     4561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4741 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5281 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5341 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5401 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     5461 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     5521 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5581 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5641 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     5701 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     5761 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     5821 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     5881 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     5941 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     6001 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     6061 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     6121 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     6181 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     6241 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     6301 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     6361 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     6421 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     6481 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     6541 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     6601 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     6661 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     6721 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     6781 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     6841 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     6901 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     6961 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     7021 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     7081 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     7141 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     7201 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     7261 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     7321 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     7381 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     7441 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     7501 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     7561 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     7621 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     7681 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     7741 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     7801 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     7861 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     7921 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     7981 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     8041 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     8101 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     8161 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     8221 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     8281 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     8341 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     8401 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     8461 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
     8521 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
     8581 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
     8641 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
     8701 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
     8761 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
     8821 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
     8881 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
     8941 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
     9001 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
     9061 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
     9121 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
     9181 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
     9241 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
     9301 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
     9361 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
     9421 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
     9481 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
     9541 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
     9601 acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct
     9661 agcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9721 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9781 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg
     9841 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
     9901 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
     9961 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
    10021 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
    10081 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
    10141 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
    10201 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
    10261 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
    10321 ccaaagggca at
//
