LOCUS       sequence-23324-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Merritt C, Seydoux G
  TITLE     Transgenic solutions for the germline.
  JOURNAL   WormBook. 2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1.
  PUBMED    20169625
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "WormBook. 
            2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1."
COMMENT     Sequence Label: pCM1.56
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..104
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    598..721
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(794..812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(817..833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggagctcca ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcaatgc ttttttataa tgccaacttt gtatagaaaa gttgctgcag gtcgactcta
      121 gaggatcaag agcatttgaa tcagaatatg gagaacggag catgagcatt ttcgaagttt
      181 tttagatgca ctagaacaaa gcgtgttggc ttcctctgag cccgctttcc ttatataccc
      241 gcattctgca gccttacaga atgttctaga aggtcctaga tgcattcgtt tgaaaatact
      301 cccggtgggt gcaaagagac gcagacggaa aatgtatctg ggtctcttta atgtgtacac
      361 tacttttcca tgtaccgaat gtgagtcgcc ctccttttgc aacaagcagc tcgaatgttc
      421 tagaaaaagg tggaaaatag tataaatacc gttgaaaata aataccgaac aacatttgct
      481 ctaattgtga aattagaaat cttcaaacta taatcatctc actggatcct atcgattcgc
      541 ggccgctgta cacccgggtg ccctatagtg agtcgtattg gtaccggtag aaaaaatcaa
      601 gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      661 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
      721 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
      781 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      841 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      901 acaataaaac tgtctgctta cataaacagt aa
//
