LOCUS       sequence-233241-       10377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mKO2 under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP607
FEATURES             Location/Qualifiers
     source          1..10377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(146..157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2097..2114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2098..2114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2139..2738
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             2751..3407
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(4193..4209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4193..4209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4216..4240)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5668..5688)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(5716..5912)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5918..6733)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5918..6733)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6641..6660
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(6743..6765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6754..6770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6779..6784,6785..6800,6801..6806))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      7161..7749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7650..7669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      7812..8974
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8997..9017
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10353..10377
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
       61 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
      121 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
      181 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
      241 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
      301 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
      361 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
      421 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
      481 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
      541 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
      601 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
      661 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
      721 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
      781 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
      841 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
      901 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
      961 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     1021 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     1081 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     1141 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
     1201 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
     1261 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
     1321 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
     1381 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     1441 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     1501 tgtggagttc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     1561 cttcaaagat cgccttctga agtgttttga ctttgaattt ggattctgta ttccgaactc
     1621 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     1681 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     1741 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     1801 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
     1861 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
     1921 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
     1981 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
     2041 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg cacgggtgta
     2101 aaacgacggc cagtccgtaa tacgactcac ttaaggccgc acctttggtc ttttattgtc
     2161 aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat aaaataaaga
     2221 cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga gaaaaagtat
     2281 gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg ctaggagtta
     2341 gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa attaacgtga
     2401 gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg tttttatctt
     2461 tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt caaacattca
     2521 gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg ctttcaacaa
     2581 atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt ttaaaaatat
     2641 tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc actcttctta
     2701 cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctctaga atggtctccg
     2761 tcatcaagcc agagatgaag atgcgttact acatggacgg ctccgtcaac ggacacgagt
     2821 tcacaatcga gggagaggga accggacgtc catacgaggg acaccaagag atgaccctcc
     2881 gtgtcaccat ggccgaggga gggccaatgc cattcgcctt cgacctcgtg tcccacgtct
     2941 tctgctacgg acaccgtgtc ttcaccaagt acccagagga gatcccagac tacttcaagc
     3001 aagccttccc agagggactc tcctgggagc gttccctcga gttcgaggac ggaggatccg
     3061 cctccgtctc cgcgcacatc tccctccgtg gaaacacctt ctaccacaag tccaagttca
     3121 ccggagtcaa cttcccagcc gacggaccaa tcatgcaaaa ccaatccgtc gactgggagc
     3181 catccaccga gaagatcacc gcctccgacg gagtcctcaa gggagacgtc accatgtacc
     3241 tcaagctcga gggaggagga aaccacaagt gccaaatgaa gaccacctac aaggccgcca
     3301 aggagatcct cgagatgcca ggagaccact acatcggaca ccgtctcgtc cgtaagaccg
     3361 agggaaacat caccgagcaa gtcgaggacg ccgtcgccca cagctaaggc cgctgtcatc
     3421 agacatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     3481 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     3541 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     3601 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     3661 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     3721 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     3781 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     3841 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     3901 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     3961 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     4021 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     4081 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
     4141 cataactgtt tgcccactag tgagtcgtat tacgtagctt ggcgtaatca tggtcatagc
     4201 tgtttcctgc ccgtgaccca gctttcttgt acaaagtggt aagtgcaagt aagatcagtg
     4261 tttgtttcga tatcagtctg tttcgtaacg gtcttctgta taactacaaa aaagaataaa
     4321 aaaccgtatc acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa
     4381 ttattttgta aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa
     4441 ctaacgtgtt ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa
     4501 ttgatatttc aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg
     4561 tgagtttaca tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa
     4621 acccatttct aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt
     4681 tgtagctttt ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt
     4741 tctagtaaga aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa
     4801 aaagtatatt acacaattca agttacatta ttttcaggaa acaatggcga agagcaaagc
     4861 agagattcaa aaggaggaaa gagacaacag atatgaacga agacttcttg aaaagagaaa
     4921 gagacatgag gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa
     4981 ttctggagtt aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa
     5041 taggggaata gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct
     5101 gcagggccgt tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt
     5161 atccagaatt tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca
     5221 tacaattttg tagatcgaca acaaggcctc cacttcttca tcgatggcct ctgcagcacc
     5281 tgcaccacca agtttgtatt tttcatatta atataaagtg agccacgcat agtcgttttt
     5341 aggatttttg ccagatcgat gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg
     5401 cgtgacatgg gccacggatt gtaacgccgc gctggatgga taaaaattta ttcagttttt
     5461 tagcctttac aaaatatggt tcgactactt tctttatttt tacattttta taggtgctca
     5521 ttgagctgag atattttggg tataatcgga aaatcgctga ttttggtcat ttttctgcac
     5581 taaagattac acaaaaagtt cagtatttag attcattgtt gaaaattggg gatgatctac
     5641 agatacatat cgccgtgttc aaaaacacaa ctttattata catagttgat aattcgatgg
     5701 tatcagctca ctcaaagtat agcgaccagc attcacatac gattgacgca tgatattact
     5761 ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc
     5821 acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag
     5881 ttcttgaaaa caagaatctt tttattgtca gtactgatta gaaaaactca tcgagcatca
     5941 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     6001 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     6061 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     6121 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     6181 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     6241 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     6301 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     6361 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     6421 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     6481 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     6541 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     6601 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     6661 catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt
     6721 gaatatggct cattttatgt ttcctgtgtg aaattgttat ccgctcacaa ttccacagat
     6781 tatacgagcc gatgattaat tgtcaacagc tctttatgtt cggatgtgat gtgagaactg
     6841 tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg caaatcctaa
     6901 ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc tgtgagggga
     6961 gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg cgccgtgcgg
     7021 ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta aatgtacggg
     7081 cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag ggtattctgg
     7141 gcctccatgt cgggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccgaaa
     7801 agtgccacct gacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc
     7861 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa
     7921 agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg
     7981 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     8041 gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca
     8101 tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg
     8161 ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag
     8221 gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta
     8281 ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt
     8341 ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt
     8401 cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga
     8461 aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt
     8521 tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc
     8581 aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat
     8641 agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct
     8701 cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt
     8761 tcaaaagcgc tctgaagttc ctatactttc tagctagaga ataggaactt cggaatagga
     8821 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     8881 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     8941 agaacggcat agtgcgtgtt tatgcttaaa tgcgcgccag ggatccgcca agctatcaac
     9001 tttgtataga aaagttgtgt cggattatgg gctcttcttt tatcctctac tttctttcta
     9061 gaaaatgcaa atgttgagtc agaaattgct gaatggagta caactgcaat gggtttagct
     9121 tatgtttctg gaacaattac tggtggaatt gtgattgaaa agtaaggtta tatttcattt
     9181 caataaatgt taccacagta taacaaaaac aagttacatt gttcagagta tgtctgaatg
     9241 tattattatt attgtatttg tgcatcagta ctgtagttgt actgaagaag ggtttaaaat
     9301 aatctcaacg tttttaagac gagttagtga gaatacggtc gcagttagga tgtttggaca
     9361 tagttttatt agataatcgt gaactaactc actataaaaa cccgctaact cattgaataa
     9421 gatagtttta aacgttcaaa atttgaagac aaaattgtat gaataagttt acagagttgg
     9481 tcgacgcaag cttctccttc tcttcacatt tctcaataat ttagctctaa ttgcattcgt
     9541 attcttcgca aaaatacgta ttctgattga cccgatgaaa tacggatgcc tagttgcatt
     9601 gatcatctac ggttatactt atgggtgagg tttttcaaat atattttgat ttaatcgaat
     9661 aatgaaattt cagaactgga gttggtccaa tttcgtggtt tatatcctct gaactggtac
     9721 ctcaaaaaca tcgtagtatt gctcaatctg tggcatattc aatgaatact gtaatggtgg
     9781 ttatcagtac atttacagta ttacctctat attccgttat tggtagttat gctttcctta
     9841 ttctatattc aattccttca tttataagta tgcttatatt atttcgctac ttaccggaaa
     9901 ccaaaggacg agagattcat gatattgtga atgaacttaa aaaaaaatga aaacctttga
     9961 aaacgttcat acggccgaaa ttttacttga tcgagccaat tgaacttttg ttaagactta
    10021 cagaaaagtg agagattttg ttacaaaaaa gtgagaaaaa agcgaaaatg aacaggaaaa
    10081 agaagaaaaa tacacccgaa gtatcatata aaaattaaaa agattgtttg acctggcgga
    10141 acttttttgc aaaaactctg aatatccatg gcacatgctt cgtgcaaaac aggatccaaa
    10201 tgaatatcaa caagagattc ttgaagacga cgagccactt gctcagagca ttgcttatct
    10261 cgaatgagac ccttgttaaa atcatttttc aaacattata tgacattgtc gaaatgtcct
    10321 cctgattcca tgatggtagc aaactcactt cgtcaagttt gtacaaaaaa gcaggct
//
