LOCUS       sequence-233243-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mTFP under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP609
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(146..157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2097..2114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2098..2114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2139..2738
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     complement(4247..4263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4247..4263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4270..4294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5722..5742)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(5770..5966)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5972..6787)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5972..6787)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6695..6714
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(6797..6819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6808..6824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6833..6838,6839..6854,6855..6860))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      7215..7803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7704..7723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      7866..9028
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9051..9071
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10407..10431
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
       61 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
      121 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
      181 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
      241 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
      301 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
      361 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
      421 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
      481 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
      541 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
      601 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
      661 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
      721 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
      781 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
      841 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
      901 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
      961 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     1021 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     1081 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     1141 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
     1201 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
     1261 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
     1321 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
     1381 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     1441 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     1501 tgtggagttc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     1561 cttcaaagat cgccttctga agtgttttga ctttgaattt ggattctgta ttccgaactc
     1621 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     1681 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     1741 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     1801 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
     1861 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
     1921 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
     1981 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
     2041 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg cacgggtgta
     2101 aaacgacggc cagtccgtaa tacgactcac ttaaggccgc acctttggtc ttttattgtc
     2161 aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat aaaataaaga
     2221 cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga gaaaaagtat
     2281 gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg ctaggagtta
     2341 gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa attaacgtga
     2401 gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg tttttatctt
     2461 tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt caaacattca
     2521 gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg ctttcaacaa
     2581 atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt ttaaaaatat
     2641 tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc actcttctta
     2701 cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctctaga atggtttcta
     2761 agggagagga gaccaccatg ggagtcatca agccagacat gaagatcaag ctcaagatgg
     2821 agggaaacgt caacggacac gccttcgtca tcgagggaga gggagaggga aagccatacg
     2881 acggaaccaa cactatcaac ctcgaggtca aggaaggagc cccactccca ttctcctacg
     2941 acatcctcac caccgcgttc gcctacggaa accgtgcctt caccaagtac ccagacgaca
     3001 tcccaaacta ctttaagcaa tccttcccag agggatactc ctgggagcgt accatgacct
     3061 tcgaagacaa gggaatcgtc aaggtcaagt cagacatctc catggaggag gactccttca
     3121 tctacgagat ccacctcaag ggagagaact tcccaccaaa cggaccagtc atgcaaaaga
     3181 agaccaccgg atgggacgcc tccaccgaga gaatgtacgt ccgtgacgga gtcctcaagg
     3241 gagacgtcaa gcacaagttg ctcctcgagg gaggaggaca ccaccgtgtc gactttaaga
     3301 ccatctaccg tgccaagaag gccgtcaagc tcccagacta ccacttcgtc gaccaccgta
     3361 tagagatcct caaccacgac aaggactaca acaaggtcac cgtctacgag tccgccgtcg
     3421 cccgtaactc taccgacgga atggatgagc tctacaagta aggccgctgt catcagacat
     3481 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     3541 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     3601 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     3661 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     3721 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     3781 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     3841 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
     3901 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
     3961 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
     4021 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     4081 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     4141 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     4201 tgtttgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
     4261 ctgcccgtga cccagctttc ttgtacaaag tggtaagtgc aagtaagatc agtgtttgtt
     4321 tcgatatcag tctgtttcgt aacggtcttc tgtataacta caaaaaagaa taaaaaaccg
     4381 tatcacaact gagaaaaaag acaaaactta ttgaacagtg ttcctttcaa aaaattattt
     4441 tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat caaaaacaag aaaactaacg
     4501 tgttctcctg ggcgtggaac tccttatcaa gattaccatt attgtctgtc agaattgata
     4561 tttcaattat ttcatatcta gacaaacccg ggtgtcttgc atttattagt actgtgagtt
     4621 tacatacaaa cgaggcttac tcagataaca tagagtcacc tctaattagc aaaaacccat
     4681 ttctaattag ctatttcgac cagttctaaa gtcaccgtaa ttagaaatgg tttttgtagc
     4741 ttttctaatt agaaatgacc ccgagataag tgaaatatgg cattttctgg gttttctagt
     4801 aagaaatgat cccgcacgta gtgattccgc gttacaagcc aaattcaaat taaaaaagta
     4861 tattacacaa ttcaagttac attattttca ggaaacaatg gcgaagagca aagcagagat
     4921 tcaaaaggag gaaagagaca acagatatga acgaagactt cttgaaaaga gaaagagaca
     4981 tgaggtgagt aaccatgata ataaataatc aagtaagggc tatagattta taaattctgg
     5041 agttaacagt ttcgaaaaaa agtagttttc atatgtgcga aggaatatca aaaatagggg
     5101 aatagcctaa acgagtgaga aaattaaatg tttgttattt tttcaaaaat gtctgcaggg
     5161 ccgttatcct ttcaaatgca ctctatcaaa catagatcat atccatcata tcttatccag
     5221 aatttatatc atatccaaga ttgagagcgt ttcaaggaaa atctcaaaca accatacaat
     5281 tttgtagatc gacaacaagg cctccacttc ttcatcgatg gcctctgcag cacctgcacc
     5341 accaagtttg tatttttcat attaatataa agtgagccac gcatagtcgt ttttaggatt
     5401 tttgccagat cgatgtcgcg caaacagact gttttttttt ctttgaaagc cgtgcgtgac
     5461 atgggccacg gattgtaacg ccgcgctgga tggataaaaa tttattcagt tttttagcct
     5521 ttacaaaata tggttcgact actttcttta tttttacatt tttataggtg ctcattgagc
     5581 tgagatattt tgggtataat cggaaaatcg ctgattttgg tcatttttct gcactaaaga
     5641 ttacacaaaa agttcagtat ttagattcat tgttgaaaat tggggatgat ctacagatac
     5701 atatcgccgt gttcaaaaac acaactttat tatacatagt tgataattcg atggtatcag
     5761 ctcactcaaa gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
     5821 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
     5881 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
     5941 aaaacaagaa tctttttatt gtcagtactg attagaaaaa ctcatcgagc atcaaatgaa
     6001 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     6061 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     6121 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     6181 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     6241 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     6301 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     6361 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     6421 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     6481 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     6541 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     6601 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     6661 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     6721 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     6781 ggctcatttt atgtttcctg tgtgaaattg ttatccgctc acaattccac agattatacg
     6841 agccgatgat taattgtcaa cagctcttta tgttcggatg tgatgtgaga actgtatcct
     6901 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
     6961 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
     7021 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
     7081 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
     7141 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
     7201 atgtcgggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7321 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc gaaaagtgcc
     7861 acctgacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     7921 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     7981 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     8041 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     8101 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     8161 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     8221 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     8281 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     8341 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     8401 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     8461 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     8521 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     8581 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     8641 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     8701 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     8761 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
     8821 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca
     8881 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     8941 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     9001 gcatagtgcg tgtttatgct taaatgcgcg ccagggatcc gccaagctat caactttgta
     9061 tagaaaagtt gtgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat
     9121 gcaaatgttg agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt
     9181 tctggaacaa ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa
     9241 atgttaccac agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat
     9301 tattattgta tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc
     9361 aacgttttta agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt
     9421 tattagataa tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt
     9481 tttaaacgtt caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg
     9541 caagcttctc cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt
     9601 cgcaaaaata cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat
     9661 ctacggttat acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa
     9721 atttcagaac tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa
     9781 aacatcgtag tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca
     9841 gtacatttac agtattacct ctatattccg ttattggtag ttatgctttc cttattctat
     9901 attcaattcc ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag
     9961 gacgagagat tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt
    10021 tcatacggcc gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa
    10081 agtgagagat tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga
    10141 aaaatacacc cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt
    10201 ttgcaaaaac tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata
    10261 tcaacaagag attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg
    10321 agacccttgt taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat
    10381 tccatgatgg tagcaaactc acttcgtcaa gtttgtacaa aaaagcaggc t
//
