LOCUS       sequence-233244-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mtagBFP2 under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP610
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(146..157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2097..2114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2098..2114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2139..2738
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     complement(4250..4266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4250..4266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4273..4297)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5725..5745)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(5773..5969)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5975..6790)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5975..6790)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6698..6717
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(6800..6822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6811..6827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6836..6841,6842..6857,6858..6863))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      7218..7806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7707..7726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      7869..9031
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9054..9074
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10410..10434
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
       61 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
      121 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
      181 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
      241 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
      301 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
      361 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
      421 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
      481 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
      541 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
      601 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
      661 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
      721 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
      781 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
      841 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
      901 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
      961 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     1021 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     1081 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     1141 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
     1201 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
     1261 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
     1321 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
     1381 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     1441 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     1501 tgtggagttc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     1561 cttcaaagat cgccttctga agtgttttga ctttgaattt ggattctgta ttccgaactc
     1621 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     1681 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     1741 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     1801 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
     1861 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
     1921 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
     1981 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
     2041 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg cacgggtgta
     2101 aaacgacggc cagtccgtaa tacgactcac ttaaggccgc acctttggtc ttttattgtc
     2161 aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat aaaataaaga
     2221 cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga gaaaaagtat
     2281 gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg ctaggagtta
     2341 gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa attaacgtga
     2401 gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg tttttatctt
     2461 tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt caaacattca
     2521 gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg ctttcaacaa
     2581 atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt ttaaaaatat
     2641 tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc actcttctta
     2701 cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctctaga atggtctcca
     2761 agggagagga gcttatcaag gagaacatgc acatgaagct ctacatggag ggaaccgttg
     2821 acaaccacca cttcaagtgc acctccgagg gagagggaaa gccatacgag ggaacccaaa
     2881 ccatgcgtat caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca
     2941 cctccttcct ctacggttcc aagaccttca tcaaccacac ccaaggaatc ccagacttct
     3001 tcaagcaatc cttcccagag ggattcacct gggagcgtgt caccacctac gaggacggag
     3061 gagtcctcac cgccacccaa gacacctccc tccaagacgg atgcctcatc tacaacgtca
     3121 agatccgtgg agtcaacttc acctccaacg gaccagtcat gcaaaagaag accctcggat
     3181 gggaggcctt caccgagacc ctctacccag ccgacggagg acttgaggga cgtaacgaca
     3241 tggccctcaa gctcgtcgga ggatctcacc tcatcgccaa cgccaagacc acctaccgtt
     3301 ccaagaagcc agccaagaac ctcaagatgc caggagtcta ctacgtcgat taccgtcttg
     3361 agcgtatcaa ggaggccaac aacgagacct acgtcgagca acacgaggtc gccgtcgccc
     3421 gttactgcga cctcccatcc aagctcggac acaagctcaa ctaaggccgc tgtcatcaga
     3481 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     3541 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     3601 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     3661 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     3721 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
     3781 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
     3841 cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca
     3901 tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat
     3961 gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga
     4021 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     4081 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     4141 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     4201 aactgtttgc ccactagtga gtcgtattac gtagcttggc gtaatcatgg tcatagctgt
     4261 ttcctgcccg tgacccagct ttcttgtaca aagtggtaag tgcaagtaag atcagtgttt
     4321 gtttcgatat cagtctgttt cgtaacggtc ttctgtataa ctacaaaaaa gaataaaaaa
     4381 ccgtatcaca actgagaaaa aagacaaaac ttattgaaca gtgttccttt caaaaaatta
     4441 ttttgtaaaa aatcgagttt ttctccaaaa taacgcacgc aatcaaaaac aagaaaacta
     4501 acgtgttctc ctgggcgtgg aactccttat caagattacc attattgtct gtcagaattg
     4561 atatttcaat tatttcatat ctagacaaac ccgggtgtct tgcatttatt agtactgtga
     4621 gtttacatac aaacgaggct tactcagata acatagagtc acctctaatt agcaaaaacc
     4681 catttctaat tagctatttc gaccagttct aaagtcaccg taattagaaa tggtttttgt
     4741 agcttttcta attagaaatg accccgagat aagtgaaata tggcattttc tgggttttct
     4801 agtaagaaat gatcccgcac gtagtgattc cgcgttacaa gccaaattca aattaaaaaa
     4861 gtatattaca caattcaagt tacattattt tcaggaaaca atggcgaaga gcaaagcaga
     4921 gattcaaaag gaggaaagag acaacagata tgaacgaaga cttcttgaaa agagaaagag
     4981 acatgaggtg agtaaccatg ataataaata atcaagtaag ggctatagat ttataaattc
     5041 tggagttaac agtttcgaaa aaaagtagtt ttcatatgtg cgaaggaata tcaaaaatag
     5101 gggaatagcc taaacgagtg agaaaattaa atgtttgtta ttttttcaaa aatgtctgca
     5161 gggccgttat cctttcaaat gcactctatc aaacatagat catatccatc atatcttatc
     5221 cagaatttat atcatatcca agattgagag cgtttcaagg aaaatctcaa acaaccatac
     5281 aattttgtag atcgacaaca aggcctccac ttcttcatcg atggcctctg cagcacctgc
     5341 accaccaagt ttgtattttt catattaata taaagtgagc cacgcatagt cgtttttagg
     5401 atttttgcca gatcgatgtc gcgcaaacag actgtttttt tttctttgaa agccgtgcgt
     5461 gacatgggcc acggattgta acgccgcgct ggatggataa aaatttattc agttttttag
     5521 cctttacaaa atatggttcg actactttct ttatttttac atttttatag gtgctcattg
     5581 agctgagata ttttgggtat aatcggaaaa tcgctgattt tggtcatttt tctgcactaa
     5641 agattacaca aaaagttcag tatttagatt cattgttgaa aattggggat gatctacaga
     5701 tacatatcgc cgtgttcaaa aacacaactt tattatacat agttgataat tcgatggtat
     5761 cagctcactc aaagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc
     5821 tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg
     5881 ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc
     5941 ttgaaaacaa gaatcttttt attgtcagta ctgattagaa aaactcatcg agcatcaaat
     6001 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     6061 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     6121 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     6181 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     6241 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     6301 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     6361 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     6421 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     6481 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     6541 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     6601 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     6661 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     6721 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     6781 tatggctcat tttatgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacagattat
     6841 acgagccgat gattaattgt caacagctct ttatgttcgg atgtgatgtg agaactgtat
     6901 cctagcaaga ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct
     6961 tttatatttc tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg
     7021 tttccctgct cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca
     7081 tcaaaatgta tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga
     7141 cagtcacatc atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc
     7201 tccatgtcgg gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccgaaaagt
     7861 gccacctgac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct
     7921 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc
     7981 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga
     8041 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     8101 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc
     8161 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc
     8221 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct
     8281 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg
     8341 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta
     8401 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat
     8461 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat
     8521 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt
     8581 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
     8641 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
     8701 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt
     8761 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
     8821 aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact
     8881 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     8941 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     9001 acggcatagt gcgtgtttat gcttaaatgc gcgccaggga tccgccaagc tatcaacttt
     9061 gtatagaaaa gttgtgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     9121 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     9181 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     9241 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     9301 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     9361 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     9421 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     9481 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     9541 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     9601 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     9661 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     9721 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     9781 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     9841 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     9901 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     9961 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
    10021 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
    10081 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
    10141 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
    10201 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
    10261 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
    10321 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
    10381 gattccatga tggtagcaaa ctcacttcgt caagtttgta caaaaaagca ggct
//
