LOCUS       sequence-233245-       10455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression vector for mtagRFP-T under EFT3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
  TITLE     A toolkit for DNA assembly, genome engineering and multicolor 
            imaging for C. elegans.
  JOURNAL   Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. 
            Epub 2018 Apr 13.
  PUBMED    33693300
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl Med 
            Aging."; date: "2018-01"; volume: "2:1-10. doi"; pages: " 
            10.1016/j.tma.2018.01.001. Epub 2018 Apr 13"
COMMENT     Sequence Label: BSP611
FEATURES             Location/Qualifiers
     source          1..10455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(146..157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2097..2114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2098..2114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2139..2738
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     primer_bind     complement(4271..4287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4271..4287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4294..4318)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5746..5766)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(5794..5990)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5996..6811)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5996..6811)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6719..6738
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(6821..6843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6832..6848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6857..6862,6863..6878,6879..6884))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     rep_origin      7239..7827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7728..7747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      7890..9052
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9075..9095
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10431..10455
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
       61 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
      121 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
      181 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
      241 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
      301 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
      361 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
      421 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
      481 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
      541 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
      601 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
      661 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
      721 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
      781 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
      841 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
      901 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
      961 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     1021 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     1081 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     1141 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
     1201 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
     1261 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
     1321 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
     1381 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     1441 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     1501 tgtggagttc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     1561 cttcaaagat cgccttctga agtgttttga ctttgaattt ggattctgta ttccgaactc
     1621 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     1681 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     1741 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     1801 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
     1861 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
     1921 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
     1981 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
     2041 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg cacgggtgta
     2101 aaacgacggc cagtccgtaa tacgactcac ttaaggccgc acctttggtc ttttattgtc
     2161 aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat aaaataaaga
     2221 cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga gaaaaagtat
     2281 gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg ctaggagtta
     2341 gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa attaacgtga
     2401 gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg tttttatctt
     2461 tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt caaacattca
     2521 gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg ctttcaacaa
     2581 atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt ttaaaaatat
     2641 tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc actcttctta
     2701 cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctctaga atggtctcca
     2761 agggagagga gcttatcaag gagaacatgc acatgaagct ctacatggag ggaaccgtca
     2821 acaaccacca cttcaagtgc acctccgagg gagagggaaa gccatacgag ggaacccaaa
     2881 ccatgcgtat caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca
     2941 cctccttcat gtacggatcg cgtaccttca tcaaccacac ccaaggaatc ccagacttct
     3001 tcaagcaatc cttcccagag ggattcacct gggagcgtgt caccacctac gaggacggag
     3061 gagtcctcac cgccacccaa gacacctccc tccaagacgg atgcctcatc tacaacgtca
     3121 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat
     3181 gggaggccaa caccgagatg ctctacccag ccgacggagg acttgaggga cgtaccgaca
     3241 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cttcaagacc acctaccgtt
     3301 ccaagaagcc agccaagaac ctcaagatgc caggagtcta ctacgttgac caccgtcttg
     3361 agcgtatcaa ggaggccgac aaggagacct acgtcgagca acacgaggtc gccgtcgccc
     3421 gttactgcga cctcccatcc aagctcggac acaagctcaa cggaatggac gagctttaca
     3481 agtaaggccg ctgtcatcag acatctcgcg cccgtgcctc tgacttctaa gtccaattac
     3541 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     3601 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     3661 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     3721 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     3781 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     3841 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     3901 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     3961 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     4021 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     4081 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     4141 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     4201 cattcccaat ataccaaaca taactgtttg cccactagtg agtcgtatta cgtagcttgg
     4261 cgtaatcatg gtcatagctg tttcctgccc gtgacccagc tttcttgtac aaagtggtaa
     4321 gtgcaagtaa gatcagtgtt tgtttcgata tcagtctgtt tcgtaacggt cttctgtata
     4381 actacaaaaa agaataaaaa accgtatcac aactgagaaa aaagacaaaa cttattgaac
     4441 agtgttcctt tcaaaaaatt attttgtaaa aaatcgagtt tttctccaaa ataacgcacg
     4501 caatcaaaaa caagaaaact aacgtgttct cctgggcgtg gaactcctta tcaagattac
     4561 cattattgtc tgtcagaatt gatatttcaa ttatttcata tctagacaaa cccgggtgtc
     4621 ttgcatttat tagtactgtg agtttacata caaacgaggc ttactcagat aacatagagt
     4681 cacctctaat tagcaaaaac ccatttctaa ttagctattt cgaccagttc taaagtcacc
     4741 gtaattagaa atggtttttg tagcttttct aattagaaat gaccccgaga taagtgaaat
     4801 atggcatttt ctgggttttc tagtaagaaa tgatcccgca cgtagtgatt ccgcgttaca
     4861 agccaaattc aaattaaaaa agtatattac acaattcaag ttacattatt ttcaggaaac
     4921 aatggcgaag agcaaagcag agattcaaaa ggaggaaaga gacaacagat atgaacgaag
     4981 acttcttgaa aagagaaaga gacatgaggt gagtaaccat gataataaat aatcaagtaa
     5041 gggctataga tttataaatt ctggagttaa cagtttcgaa aaaaagtagt tttcatatgt
     5101 gcgaaggaat atcaaaaata ggggaatagc ctaaacgagt gagaaaatta aatgtttgtt
     5161 attttttcaa aaatgtctgc agggccgtta tcctttcaaa tgcactctat caaacataga
     5221 tcatatccat catatcttat ccagaattta tatcatatcc aagattgaga gcgtttcaag
     5281 gaaaatctca aacaaccata caattttgta gatcgacaac aaggcctcca cttcttcatc
     5341 gatggcctct gcagcacctg caccaccaag tttgtatttt tcatattaat ataaagtgag
     5401 ccacgcatag tcgtttttag gatttttgcc agatcgatgt cgcgcaaaca gactgttttt
     5461 ttttctttga aagccgtgcg tgacatgggc cacggattgt aacgccgcgc tggatggata
     5521 aaaatttatt cagtttttta gcctttacaa aatatggttc gactactttc tttattttta
     5581 catttttata ggtgctcatt gagctgagat attttgggta taatcggaaa atcgctgatt
     5641 ttggtcattt ttctgcacta aagattacac aaaaagttca gtatttagat tcattgttga
     5701 aaattgggga tgatctacag atacatatcg ccgtgttcaa aaacacaact ttattataca
     5761 tagttgataa ttcgatggta tcagctcact caaagtatag cgaccagcat tcacatacga
     5821 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
     5881 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
     5941 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgattaga
     6001 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     6061 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     6121 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     6181 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     6241 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     6301 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     6361 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     6421 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     6481 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     6541 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     6601 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     6661 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     6721 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     6781 agcaagacgt ttcccgttga atatggctca ttttatgttt cctgtgtgaa attgttatcc
     6841 gctcacaatt ccacagatta tacgagccga tgattaattg tcaacagctc tttatgttcg
     6901 gatgtgatgt gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac
     6961 ctcagtggca aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc
     7021 aacgcgtctg tgaggggagc gtttccctgc tcgcaggtct gcagcgagga gccgtaattt
     7081 ttgcttcgcg ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt
     7141 atgggctaaa tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg
     7201 tcaaggaggg tattctgggc ctccatgtcg ggatcttctt gagatccttt ttttctgcgc
     7261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7381 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7861 gccttttgct ggccgaaaag tgccacctga cgaagcatct gtgcttcatt ttgtagaaca
     7921 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     7981 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     8041 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     8101 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     8161 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     8221 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     8281 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     8341 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     8401 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     8461 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     8521 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     8581 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     8641 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     8701 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     8761 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     8821 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     8881 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     8941 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     9001 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgcgccaggg
     9061 atccgccaag ctatcaactt tgtatagaaa agttgtgtcg gattatgggc tcttctttta
     9121 tcctctactt tctttctaga aaatgcaaat gttgagtcag aaattgctga atggagtaca
     9181 actgcaatgg gtttagctta tgtttctgga acaattactg gtggaattgt gattgaaaag
     9241 taaggttata tttcatttca ataaatgtta ccacagtata acaaaaacaa gttacattgt
     9301 tcagagtatg tctgaatgta ttattattat tgtatttgtg catcagtact gtagttgtac
     9361 tgaagaaggg tttaaaataa tctcaacgtt tttaagacga gttagtgaga atacggtcgc
     9421 agttaggatg tttggacata gttttattag ataatcgtga actaactcac tataaaaacc
     9481 cgctaactca ttgaataaga tagttttaaa cgttcaaaat ttgaagacaa aattgtatga
     9541 ataagtttac agagttggtc gacgcaagct tctccttctc ttcacatttc tcaataattt
     9601 agctctaatt gcattcgtat tcttcgcaaa aatacgtatt ctgattgacc cgatgaaata
     9661 cggatgccta gttgcattga tcatctacgg ttatacttat gggtgaggtt tttcaaatat
     9721 attttgattt aatcgaataa tgaaatttca gaactggagt tggtccaatt tcgtggttta
     9781 tatcctctga actggtacct caaaaacatc gtagtattgc tcaatctgtg gcatattcaa
     9841 tgaatactgt aatggtggtt atcagtacat ttacagtatt acctctatat tccgttattg
     9901 gtagttatgc tttccttatt ctatattcaa ttccttcatt tataagtatg cttatattat
     9961 ttcgctactt accggaaacc aaaggacgag agattcatga tattgtgaat gaacttaaaa
    10021 aaaaatgaaa acctttgaaa acgttcatac ggccgaaatt ttacttgatc gagccaattg
    10081 aacttttgtt aagacttaca gaaaagtgag agattttgtt acaaaaaagt gagaaaaaag
    10141 cgaaaatgaa caggaaaaag aagaaaaata cacccgaagt atcatataaa aattaaaaag
    10201 attgtttgac ctggcggaac ttttttgcaa aaactctgaa tatccatggc acatgcttcg
    10261 tgcaaaacag gatccaaatg aatatcaaca agagattctt gaagacgacg agccacttgc
    10321 tcagagcatt gcttatctcg aatgagaccc ttgttaaaat catttttcaa acattatatg
    10381 acattgtcga aatgtcctcc tgattccatg atggtagcaa actcacttcg tcaagtttgt
    10441 acaaaaaagc aggct
//
