LOCUS       sequence-233246-        9590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes the complete CRISPR/dCas9 machinery for 
            repressing transposition of the following bacterial Insertion 
            Sequences: IS1, IS3, IS5, and IS150..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Nyerges A, Balint B, Cseklye J, Nagy I, Pal C, Feher T
  TITLE     CRISPR-interference-based modulation of mobile genetic elements in 
            bacteria.
  JOURNAL   Synth Biol (Oxf). 2019;4(1):ysz008. doi: 10.1093/synbio/ysz008. Epub
            2019 Mar 15.
  PUBMED    31008359
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2019"; volume: "4(1):ysz008. doi"; pages: " 
            10.1093/synbio/ysz008. Epub 2019 Mar 15"
COMMENT     Sequence Label: pCRIS
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..23,24..29)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(347..425)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(721..747,748..750,751..3237,3238..3240,3241..4827)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4848..4979
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4980..5015
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5046..5081
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5112..5147
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5178..5213
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5244..5279
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   5310..5345
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(5774..5793)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7646..8305)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8220..8239
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8306..8408)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8800..8817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8934..9479)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9014..9033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa attgctaacg
       61 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
      121 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatta
      181 cgaaatcatc ctgtggagct tagtaggttt agcaagatgg cagcgcctaa atgtagaatg
      241 ataaaaggat taagagatta atttccctaa aaatgataaa acaagcgttt tgaaagcgct
      301 tgtttttttg gtttgcagtc agagtagaat agaagtatca aaaaaagcac cgactcggtg
      361 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatgct gttttgaatg
      421 gttccaacaa gattatttta taacttttat aacaaataat caaggagaaa ttcaaagaaa
      481 tttatcagcc ataaaacaat acttaatact atagaatgat aacaaaataa actacttttt
      541 aaaagaattt tgtgttataa tctatttatt attaagtatt gggtaatatt ttttgaagag
      601 atattttgaa aaagaaaaat taaagcatat taaactaatt tcggaggtca ttaaaactat
      661 tattgaaatc atcaaactca ttatggattt aatttaaact ttttatttta ggaggcaaaa
      721 atggataaga aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg
      781 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc
      841 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa
      901 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt
      961 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga
     1021 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga
     1081 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa
     1141 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat
     1201 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat
     1261 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct
     1321 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga
     1381 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat
     1441 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa
     1501 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg
     1561 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt
     1621 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca
     1681 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga
     1741 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca
     1801 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta
     1861 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc
     1921 aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat
     1981 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt
     2041 gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt
     2101 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa
     2161 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa
     2221 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt
     2281 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt
     2341 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc
     2401 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt
     2461 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt
     2521 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt
     2581 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct
     2641 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga
     2701 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta
     2761 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat
     2821 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta
     2881 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact
     2941 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt
     3001 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt
     3061 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct
     3121 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga
     3181 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc
     3241 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct
     3301 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa
     3361 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta
     3421 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa
     3481 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat
     3541 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct
     3601 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat
     3661 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa
     3721 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa
     3781 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct
     3841 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc
     3901 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt
     3961 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta
     4021 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt
     4081 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct
     4141 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt
     4201 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac
     4261 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa
     4321 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta
     4381 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt
     4441 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag
     4501 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt
     4561 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa
     4621 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct
     4681 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa
     4741 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt
     4801 gatttgagtc agctaggagg tgactgaagt atattttaga tgaagattat ttcttaataa
     4861 ctaaaaatat ggtataatac tcttaataaa tgcagtaata caggggcttt tcaagactga
     4921 agtctagctg agacaaatag tgcgattacg aaatttttta gacaaaaata gtctacgagg
     4981 ttttagagct atgctgtttt gaatggtccc aaaacatgct gccaacttac tgatttagtg
     5041 tatgagtttt agagctatgc tgttttgaat ggtcccaaaa cggctccaga tgacaaacat
     5101 gatctcatat cgttttagag ctatgctgtt ttgaatggtc ccaaaacacg cggctaagtg
     5161 agtaaactct cagtcaggtt ttagagctat gctgttttga atggtcccaa aacggggcta
     5221 ttccatttca tcgtccaaca aaagttttag agctatgctg ttttgaatgg tcccaaaact
     5281 gagacctgtc tcggaagctc ataggactcg ttttagagct atgctgtttt gaatggtccc
     5341 aaaacttcag cacactgaga cttgttgagt tccatgtttt agagctatgc tgttttgaat
     5401 ggactccatt caacattgcc gatgataact tgagaaagag ggttaatacc agcagtcgga
     5461 taccttccta ttctttctgt taaagcgttt tcatgttata ataggcaaaa gaagagtagt
     5521 gtgatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     5581 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     5641 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     5701 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     5761 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     5821 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     5881 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     5941 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     6001 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     6061 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     6121 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     6181 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     6241 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     6301 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     6361 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     6421 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     6481 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     6541 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     6601 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     6661 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     6721 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     6781 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     6841 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     6901 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     6961 gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     7021 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     7081 attctgagtt acaacagtcc gcaccgctgc cggtagctcc ttccggtggg cgcggggcat
     7141 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     7201 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     7261 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
     7321 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
     7381 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
     7441 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     7501 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
     7561 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag
     7621 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     7681 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     7741 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     7801 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     7861 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     7921 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     7981 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     8041 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     8101 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     8161 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     8221 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     8281 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     8341 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     8401 gccgatcaac gtctcatttt cgccaaaagt tggcccgggg cttcccggta tcaacaggga
     8461 caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa
     8521 gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
     8581 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
     8641 aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct tactatgttg
     8701 gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt
     8761 gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg
     8821 ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga
     8881 agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
     8941 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
     9001 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
     9061 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
     9121 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
     9181 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     9241 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
     9301 tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
     9361 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
     9421 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
     9481 aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt atctcttcaa
     9541 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctaatgt
//
