LOCUS       sequence-233248-        9067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Landthaler M, Yalcin A, Tuschl T
  TITLE     The human DiGeorge syndrome critical region gene 8 and Its D. 
            melanogaster homolog are required for miRNA biogenesis.
  JOURNAL   Curr Biol. 2004 Dec 14. 14(23):2162-7.
  PUBMED    15589161
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2004 Dec 14. 14(23):2162-7."
COMMENT     Sequence Label: pcDNA4/TO/cmycDrosha
FEATURES             Location/Qualifiers
     source          1..9067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             987..1016
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5075..5092)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5081..5305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5351..5779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5438..5457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5648..5669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5788..5808)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5793..6122
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5973..6108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6035..6054
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6170..6217
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6236..6610
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6740..6861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6777..6796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6831..6850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6910..6926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6910..6926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6923..6945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6934..6950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6958..6964,6965..6982,6983..6988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7003..7024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7141..7158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7312..7900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7392..7411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8071..8862,8863..8931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8694..8713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8932..9036)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatggaac aaaaacttat ttctgaagaa gatctgatga
     1021 tgcagggaaa cacatgtcac agaatgtcgt tccacccggg acgagggtgt ccccgaggac
     1081 gaggaggaca tggagccaga ccctcagcac catcctttag gccccaaaat ctgaggctgc
     1141 ttcaccctca gcagcctcct gtgcaatatc aatatgaacc tccaagtgcc ccttccacca
     1201 ctttctcaaa ctctccagcc cccaattttc tccctccacg accagacttt gtacccttcc
     1261 ccccacccat gcctccgtca gcgcaaggcc ctcttccccc ctgcccaatc aggccgcctt
     1321 tccccaacca ccagatgagg caccccttcc cagttcctcc ttgttttcct cccatgccac
     1381 caccaatgcc ttgtcctaat aaccccccag tccctggggc acctcctgga caaggcactt
     1441 tccccttcat gatgccccct ccctccatgc ctcatccccc gccccctcca gtcatgccgc
     1501 agcaggttaa ttatcagtac cctccgggct attctcacca caacttccca cctcccagtt
     1561 ttaatagttt ccagaacaac cctagttctt tcctgcccag tgctaataac agcagtagtc
     1621 ctcatttcag acatctccct ccatacccac tcccaaaggc tcccagtgag agaaggtccc
     1681 cagaaaggct gaaacactat gatgaccaca ggcaccgaga tcacagtcat gggcgaggtg
     1741 agaggcatcg gtccctggat cggcgggagc gaggccgcag tcccgacagg agaagacaag
     1801 acagccggta cagatctgat tatgaccgag ggagaacacc atctcgccac cgcagctacg
     1861 aacggagcag agagcgagaa cgggagagac acaggcatcg agacaaccga agatcaccat
     1921 ctctggaaag gtcctacaaa aaagagtata agagatctgg aagtcgctcc ccaagtaggg
     1981 agaagaagag agctcgttgg gaggaagaaa aagaccgttg gagtgacaac cagagttctg
     2041 gcaaagacaa gaactatacc tcaatcaagg aaaaagagcc cgaggagacc atgcctgaca
     2101 agaatgagga ggaagaagaa gaacttctta agcctgtgtg gattcgatgc actcattcag
     2161 aaaactacta ctccagtgac cccatggatc aggtgggaga ttctacagtg gttggaacga
     2221 gtaggcttcg tgacttatat gacaaatttg aggaggagtt ggggagcagg caagaaaagg
     2281 ccaaagctgc tcggcctccg tgggaacctc caaagacgaa gctcgatgaa gatttagaga
     2341 gttccagtga atccgagtgt gagtctgatg aggacagcac ctgttctagc agctcagact
     2401 ctgaagtttt tgacgttatt gcagaaatca aacgcaaaaa ggcccaccct gaccgacttc
     2461 atgatgaact ttggtacaac gatccaggcc agatgaatga tggaccactc tgcaaatgca
     2521 gcgcaaaggc aagacgcaca ggaattaggc acagcattta tcctggagaa gaggccatca
     2581 agccctgtcg tcctatgacc aacaatgctg gcagactttt ccactaccgg atcacagtct
     2641 ccccgcctac gaacttttta actgacaggc caactgttat agaatacgat gatcacgagt
     2701 atatctttga aggattttct atgtttgcac atgcccccct gaccaatatt ccactgtgta
     2761 aagtaattag attcaacata gactacacga ttcatttcat tgaagagatg atgccggaga
     2821 atttttgtgt gaaagggctt gaactctttt cactgttcct attcagagat attttggaat
     2881 tatatgactg gaatcttaaa ggtcctttgt ttgaagacag ccctccctgc tgcccaagat
     2941 ttcatttcat gccacgtttt gtaagatttc ttccagatgg aggaaaggaa gtgctgtcca
     3001 tgcaccagat tctcctgtac ttgttaaggt gcagcaaagc cctggtgcct gaggaggaga
     3061 ttgccaatat gcttcagtgg gaggagctgg agtggcagaa atatgcagaa gaatgcaaag
     3121 gcatgattgt taccaaccct gggacgaaac caagctctgt ccgtatcgat caactggatc
     3181 gtgaacagtt caaccccgat gtgattactt ttccgattat cgtccacttt gggatacgcc
     3241 ctgcacagtt gagttatgca ggagacccac agtaccaaaa actgtggaag agttatgtga
     3301 aacttcgcca cctcctagca aatagtccca aagtcaaaca aactgacaaa cagaagctgg
     3361 cacagaggga ggaagccctc caaaaaatac ggcagaagaa tacaatgaga cgagaagtaa
     3421 cggtggagct aagtagccaa ggattctgga aaactggcat ccgttctgat gtctgtcagc
     3481 atgcaatgat gctacctgtt ctgacccatc atatccgcta ccaccaatgc ctaatgcatt
     3541 tggacaagtt gataggatat actttccaag atcgttgtct gttgcagctg gccatgactc
     3601 atccaagtca tcatttaaat tttggaatga atcctgatca tgccaggaat tcattatcta
     3661 actgtggaat tcggcagccc aaatacggag acagaaaagt tcatcacatg cacatgcgga
     3721 agaaagggat taacaccttg ataaatatca tgtcacgcct tggccaagat gacccaactc
     3781 cctcgaggat taaccacaat gaacggttgg aattcctggg tgatgctgtt gttgaatttc
     3841 tgaccagcgt ccatttgtac tatttgtttc ctagtctgga agaaggagga ttagcaacct
     3901 atcggactgc cattgttcag aatcagcacc ttgccatgct agcaaagaaa cttgaactgg
     3961 atcgatttat gctgtatgct cacgggcctg acctttgtag agaatcggac cttcgacatg
     4021 caatggccaa ttgttttgaa gcgttaatag gagctgttta cttggaggga agcctggagg
     4081 aagccaagca gttatttgga cgcttgctct ttaatgatcc ggacctgcgc gaagtctggc
     4141 tcaattatcc tctccaccca ctccaactac aagagccaaa tactgatcga caacttattg
     4201 aaacttctcc ggttctacaa aaacttactg agtttgaaga agcaattgga gtaattttta
     4261 ctcatgttcg acttctggca agggcattca cattgagaac tgtgggattt aaccatctga
     4321 ccctaggcca caatcagaga atggaattcc taggtgactc cataatgcaa ctggtagcca
     4381 cagagtactt attcattcat ttcccagatc atcatgaagg acacttaact ttgttgcgaa
     4441 gctctttggt gaataataga actcaggcca aggtagcgga ggagctgggc atgcaggagt
     4501 acgccataac caacgacaag accaagaggc ctgtggcgct tcgcaccaag accttggcgg
     4561 accttttgga atcatttatt gcagcgctgt acattgataa ggatttggaa tatgttcata
     4621 ctttcatgaa tgtctgcttc tttccacgat tgaaagagtt cattttgaat caggattgga
     4681 atgaccccaa atcccagctt cagcagtgtt gcttgacact taggacagaa ggaaaagagc
     4741 cagacattcc tctgtacaag actctgcaga cagtgggccc atcccatgcc cgaacctaca
     4801 ctgtggctgt ttatttcaag ggagaaagaa taggctgtgg gaaaggacca agtattcagc
     4861 aagcggaaat gggagcagca atggatgcgc ttgaaaaata taattttccc cagatggccc
     4921 atcagaagcg gttcatcgaa cggaagtaca gacaagagtt aaaagaaatg aggtgggaaa
     4981 gagagcatca agagagagag ccagatgaga ctgaagacat caagaaataa gcggccgctc
     5041 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5101 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5161 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5221 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5281 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5341 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5401 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5461 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5521 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5581 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5641 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5701 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5761 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5881 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5941 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6001 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6061 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6121 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6181 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     6241 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     6301 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     6361 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     6421 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     6481 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     6541 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     6601 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6661 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6721 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6781 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6841 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6961 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7021 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7081 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7141 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7201 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7261 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7801 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7861 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7921 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9001 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9061 tgacgtc
//
