LOCUS       sequence-23325-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Merritt C, Seydoux G
  TITLE     Transgenic solutions for the germline.
  JOURNAL   WormBook. 2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1.
  PUBMED    20169625
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "WormBook. 
            2010 Feb 8:1-21. doi: 10.1895/wormbook.1.148.1."
COMMENT     Sequence Label: pCM1.57
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..105
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    606..729
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(825..841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(825..841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg cttttttata atgccaactt tgtatagaaa agttgctaca ggtcgactct
      121 agaggatcac caaaaacgga acgttgagct ggacggaaat agtggtaaag tgacatgatt
      181 atagtttgaa gatttctaat ttcacaatta gagcaaatgt tgttcggtat ttattttcaa
      241 cggtatttat actattttcc acctttttct agaacattcg agctgcttgt tgcaaaagga
      301 gggcgactca cattcggtac atggaaaagt agtgtacaca ataaagagac ccagatacat
      361 tttccgtctg cgtctctttg cacccaccgg gagtattttc aaacgaatgc atctaggacc
      421 ttctagaaca ttctgtaagg ctgcagaatg cgggtatata aggaaagcgg gctcagagga
      481 agccaacacg ctttgttcta gtgcatctaa aaaacttcga aaatcctcat cggatcctat
      541 cgattcgcgg ccgctgtaca cccgggtgcc ctatagtgag tcgtattggt accggtagaa
      601 aaaatcaagt ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa atatcaatat
      661 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatgca
      721 gtcactatga accaactact tagatggtat tagtgacctg tagaattcga gctctagagc
      781 tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
      841 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      901 catcatgaac aataaaactg tctgcttnc
//
