LOCUS       sequence-23327-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.47
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..170)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(294..389)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(426..445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(427..445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(450..466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(450..466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(568..587)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(646..665)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tagagctcgn ttctacaggt cactaatacc atctaagtag ttggttcata gtgactgcat
       61 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      121 gatatttata tcattttacg tttctcgttc aactttcttg tacaaagtgg gataattttg
      181 ccgtattttc catattttgt tttgtatatt tatccactca ccccctctct ttgtcctgtg
      241 aatgaacttg tgccaaaaaa gccaaaaata atttactttt tttaaagaca tatcaacttt
      301 attatacaaa gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt
      361 cactatcagt caaaataaaa tcattatttg gagctccatg gtagcgttaa cgcggccgcg
      421 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      481 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      541 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      601 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      661 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
      721 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
      781 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
      841 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
      901 tagaagaata tcctgattc
//
