LOCUS       sequence-233275-        9436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses negative-feedback dLwCas13a for mammalian RNA 
            targeting/imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, 
            Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, 
            Voytas DF, Ting AY, Zhang F
  TITLE     RNA targeting with CRISPR-Cas13.
  JOURNAL   Nature. 2017 Oct 4. doi: 10.1038/nature24049.
  PUBMED    28976959
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Oct 4. doi: 10.1038/nature24049."
COMMENT     Sequence Label: pC015 - dLwCas13a-NF
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(281..1072,1073..1141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     904..923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1142..1246)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1297..1308
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1710..1729
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1781..3199,3200..3202,3203..4915,4916..4918,
                     4919..5236)
                     /codon_start=1
                     /product="catalytically dead mutant of a Leptotrichia wadei
                     CRISPR-Cas effector that acts as an RNA-guided RNAse 
                     (Abudayyeh et al., 2017)"
                     /label=dLwCas13a
                     /note="contains the inactivating mutations R474A and 
                     R1046A"
                     /translation="MKVTKVDGISHKKYIEEGKLVKSTSEENRTSERLSELLSIRLDIY
                     IKNPDNASEEENRIRRENLKKFFSNKVLHLKDSVLYLKNRKEKNAVQDKNYSEEDISEY
                     DLKNKNSFSVLKKILLNEDVNSEELEIFRKDVEAKLNKINSLKYSFEENKANYQKINEN
                     NVEKVGGKSKRNIIYDYYRESAKRNDYINNVQEAFDKLYKKEDIEKLFFLIENSKKHEK
                     YKIREYYHKIIGRKNDKENFAKIIYEEIQNVNNIKELIEKIPDMSELKKSQVFYKYYLD
                     KEELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLL
                     NKLDTYVRNCGKYNYYLQVGEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETE
                     NENGITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKQNEVKENLKMFYSYDFNMDNKNE
                     IEDFFANIDEAISSIAHGIVHFNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLK
                     IFKQLNSANVFNYYEKDVIIKYLKNTKFNFVNKNIPFVPSFTKLYNKIEDLRNTLKFFW
                     SVPKDKEEKDAQIYLLKNIYYGEFLNKFVKNSKVFFKITNEVIKINKQRNQKTGHYKYQ
                     KFENIEKTVPVEYLAIIQSREMINNQDKEEKNTYIDFIQQIFLKGFIDYLNKNNLKYIE
                     SNNNNDNNDIFSKIKIKKDNKEKYDKILKNYEKHNRNKEIPHEINEFVREIKLGKILKY
                     TENLNMFYLILKLLNHKELTNLKGSLEKYQSANKEETFSDELELINLLNLDNNRVTEDF
                     ELEANEIGKFLDFNENKIKDRKELKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLLEK
                     IADKAKYKISLKELKEYSNKKNEIEKNYTMQQNLHRKYARPKKDEKFNDEDYKEYEKAI
                     GNIQKYTHLKNKVEFNELNLLQGLLLKILHRLVGYTSIWERDLRFRLKGEFPENHYIEE
                     IFNFDNSKNVKYKSGQIVEKYINFYKELYKDNVEKRSIYSDKKVKKLKQEKKDLYIANY
                     IAHFNYIPHAEISLLEVLENLRKLLSYDRKLKNAIMKSIVDILKEYGFVATFKIGADKK
                     IEIQTLESEKIVHLKNLKKKKLMTDRNSEELCELVKVMFEYKALE"
     CDS             join(5282..5284,5285..5992)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(5282..5284,5285..5992)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(5324..5345)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5585..5604)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5932..5953
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6038..6379
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             6428..6553
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     CDS             6563..6583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6600..6732
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6715..6734
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6741..7329
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6794..6814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7212..7223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7356..7373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7362..7473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7569..7589)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7569..7587)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7608..7624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7730..7749)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7795..7850
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7849..7868)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8211..8227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8235..8251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8259..8265,8266..8283,8284..8289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8304..8325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8384..8579
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8430..8565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8585..8719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8622..8641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8676..8695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8787..8804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9038..9057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
       61 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
      481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
      541 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
      601 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
      661 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
      721 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
      781 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
      841 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
      901 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
      961 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1021 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1081 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1141 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1201 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1261 aagtgccacc tgggtcgaca ttgattattg actagagtca tcctcatcgt cgacattgat
     1321 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     1381 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1441 gcccattgac gtcaataatg acaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     1501 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     1561 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     1681 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     1741 ttttggcaaa gaattcggta ccgcgggccc gggcgccacc atgaaagtga ccaaggtcga
     1801 cggcatcagc cacaagaagt acatcgaaga gggcaagctc gtgaagtcca ccagcgagga
     1861 aaaccggacc agcgagagac tgagcgagct gctgagcatc cggctggaca tctacatcaa
     1921 gaaccccgac aacgcctccg aggaagagaa ccggatcaga agagagaacc tgaagaagtt
     1981 ctttagcaac aaggtgctgc acctgaagga cagcgtgctg tatctgaaga accggaaaga
     2041 aaagaacgcc gtgcaggaca agaactatag cgaagaggac atcagcgagt acgacctgaa
     2101 aaacaagaac agcttctccg tgctgaagaa gatcctgctg aacgaggacg tgaactctga
     2161 ggaactggaa atctttcgga aggacgtgga agccaagctg aacaagatca acagcctgaa
     2221 gtacagcttc gaagagaaca aggccaacta ccagaagatc aacgagaaca acgtggaaaa
     2281 agtgggcggc aagagcaagc ggaacatcat ctacgactac tacagagaga gcgccaagcg
     2341 caacgactac atcaacaacg tgcaggaagc cttcgacaag ctgtataaga aagaggatat
     2401 cgagaaactg tttttcctga tcgagaacag caagaagcac gagaagtaca agatccgcga
     2461 gtactatcac aagatcatcg gccggaagaa cgacaaagag aacttcgcca agattatcta
     2521 cgaagagatc cagaacgtga acaacatcaa agagctgatt gagaagatcc ccgacatgtc
     2581 tgagctgaag aaaagccagg tgttctacaa gtactacctg gacaaagagg aactgaacga
     2641 caagaatatt aagtacgcct tctgccactt cgtggaaatc gagatgtccc agctgctgaa
     2701 aaactacgtg tacaagcggc tgagcaacat cagcaacgat aagatcaagc ggatcttcga
     2761 gtaccagaat ctgaaaaagc tgatcgaaaa caaactgctg aacaagctgg acacctacgt
     2821 gcggaactgc ggcaagtaca actactatct gcaagtgggc gagatcgcca cctccgactt
     2881 tatcgcccgg aaccggcaga acgaggcctt cctgagaaac atcatcggcg tgtccagcgt
     2941 ggcctacttc agcctgagga acatcctgga aaccgagaac gagaacggta tcaccggccg
     3001 gatgcggggc aagaccgtga agaacaacaa gggcgaagag aaatacgtgt ccggcgaggt
     3061 ggacaagatc tacaatgaga acaagcagaa cgaagtgaaa gaaaatctga agatgttcta
     3121 cagctacgac ttcaacatgg acaacaagaa cgagatcgag gacttcttcg ccaatatcga
     3181 tgaggccatc tctagtattg ctcacggcat cgtgcatttc aacctggaac tggaaggcaa
     3241 ggacatcttc gccttcaaga atatcgcccc cagcgagatc tccaagaaga tgtttcagaa
     3301 cgaaatcaac gaaaagaagc tgaagctgaa aatcttcaag cagctgaaca gcgccaacgt
     3361 gttcaactac tacgagaagg atgtgatcat caagtacctg aagaatacca agttcaactt
     3421 cgtgaacaaa aacatcccct tcgtgcccag cttcaccaag ctgtacaaca agattgagga
     3481 cctgcggaat accctgaagt ttttttggag cgtgcccaag gacaaagaag agaaggacgc
     3541 ccagatctac ctgctgaaga atatctacta cggcgagttc ctgaacaagt tcgtgaaaaa
     3601 ctccaaggtg ttctttaaga tcaccaatga agtgatcaag attaacaagc agcggaacca
     3661 gaaaaccggc cactacaagt atcagaagtt cgagaacatc gagaaaaccg tgcccgtgga
     3721 atacctggcc atcatccaga gcagagagat gatcaacaac caggacaaag aggaaaagaa
     3781 tacctacatc gactttattc agcagatttt cctgaagggc ttcatcgact acctgaacaa
     3841 gaacaatctg aagtatatcg agagcaacaa caacaatgac aacaacgaca tcttctccaa
     3901 gatcaagatc aaaaaggata acaaagagaa gtacgacaag atcctgaaga actatgagaa
     3961 gcacaatcgg aacaaagaaa tccctcacga gatcaatgag ttcgtgcgcg agatcaagct
     4021 ggggaagatt ctgaagtaca ccgagaatct gaacatgttt tacctgatcc tgaagctgct
     4081 gaaccacaaa gagctgacca acctgaaggg cagcctggaa aagtaccagt ccgccaacaa
     4141 agaagaaacc ttcagcgacg agttggaact gatcaacctg ctgaacctgg acaacaacag
     4201 agtgaccgag gacttcgagc tggaagccaa cgagatcggc aagttcctgg acttcaacga
     4261 aaacaaaatc aaggaccgga aagagctgaa aaagttcgac accaacaaga tctatttcga
     4321 cggcgagaac atcatcaagc accgggcctt ctacaatatc aagaaatacg gcatgctgaa
     4381 tctgctggaa aagatcgccg ataaggccaa gtataagatc agcctgaaag aactgaaaga
     4441 gtacagcaac aagaagaatg agattgaaaa gaactacacc atgcagcaga acctgcaccg
     4501 gaagtacgcc agacccaaga aggacgaaaa gttcaacgac gaggactaca aagagtatga
     4561 gaaggccatc ggcaacatcc agaagtacac ccacctgaag aacaaggtgg aattcaatga
     4621 gctgaacctg ctgcagggcc tgctgctgaa gatcctgcac cggctcgtgg gctacaccag
     4681 catctgggag cgggacctga gattccggct gaagggcgag tttcccgaga accactacat
     4741 cgaggaaatt ttcaatttcg acaactccaa gaatgtgaag tacaaaagcg gccagatcgt
     4801 ggaaaagtat atcaacttct acaaagaact gtacaaggac aatgtggaaa agcggagcat
     4861 ctactccgac aagaaagtga agaaactgaa gcaggagaag aaagacctgt atattgcaaa
     4921 ctacatcgct cactttaatt atattcctca tgccgagatt agcctgctgg aagtgctgga
     4981 aaacctgcgg aagctgctgt cctacgaccg gaagctgaag aacgccatca tgaagtccat
     5041 cgtggacatt ctgaaagaat acggcttcgt ggccaccttc aagatcggcg ctgacaagaa
     5101 gatcgaaatc cagaccctgg aatcagagaa gatcgtgcac ctgaagaatc tgaagaaaaa
     5161 gaaactgatg accgaccgga acagcgagga actgtgcgaa ctcgtgaaag tcatgttcga
     5221 gtacaaggcc ctggaaggag gaggtggaag cggaggagga ggaagcggag gaggaggtag
     5281 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5341 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5401 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     5461 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     5521 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5581 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     5641 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     5701 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     5761 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5821 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5881 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5941 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt actctagggg
     6001 agctggcgct ggagcgggtg caggggctgg ctctagattc cagtgccgga tctgcatgcg
     6061 gaacttcagc gaccggtcca acctgagcag gcacatcaga acccacaccg gagaaaagcc
     6121 cttcgcctgc gacatttgcg gccggaagtt cgccatcagc agcaacctga acagccacac
     6181 caagatccac actggcagcc agaaaccttt ccagtgcaga atttgtatga gaaactttag
     6241 cagaagcgac aacctggcca gacacatccg gacacatact ggtgaaaaac cttttgcctg
     6301 tgatatctgt ggcagaaagt ttgccacctc cggcaatctg acccggcaca caaagattca
     6361 cctgcggggc agccagctat cgattgtcga cgctcctgaa caacgtgaag gtgcttctca
     6421 agtttctgtt tctgttactt ttgaagatgt tgctgttctt tttactcgtg atgaatggaa
     6481 aaaacttgat ctttctcaac gttctcttta tcgtgaagtt atgcttgaaa attattctaa
     6541 tcttgcttct atggctggat cccccaagaa aaagcgcaag gtataactcg aggccgcagg
     6601 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     6661 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     6721 ttctctccac aggtgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6901 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6961 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7021 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7141 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7201 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7261 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7321 cctccccgcc tggaattcga gctcggtacg atcagcctcg actgtgcctt ctagttgcca
     7381 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     7441 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     7501 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcccagc
     7561 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     7621 cctgtgtgaa attgttatcc gctaattcac tcctcaggtg caggctgcct atcagaaggt
     7681 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat ctttttccct
     7741 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7801 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7861 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7921 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     7981 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8041 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8101 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8161 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     8221 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8281 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8341 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     8401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     8581 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8641 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8701 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8821 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8881 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8941 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9001 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9061 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9121 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9181 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9241 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9301 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9361 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9421 ctgctgaagc cagtta
//
