LOCUS       sequence-233283-        5914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV vector that expresses rtTA3G under the c-fos promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, 
            Lim CS, Kaang BK
  TITLE     Interregional synaptic maps among engram cells underlie memory 
            formation.
  JOURNAL   Science. 2018 Apr 27;360(6387):430-435. doi: 
            10.1126/science.aas9204.
  PUBMED    29700265
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-04-27- 27"; volume: "360"; 
            issue: "6387"; pages: "430-435"
COMMENT     Sequence Label: pAAV-FAH-rtTA3G
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             complement(846..857)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1826..2572
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    2661..3137
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2860..2879)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3177..3317
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3177..3306
                     /label=AAV2 ITR
     rep_origin      3392..3847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3479..3498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3689..3710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3864..3883)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3983..4005
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4043..4061)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4129..4233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4234..4302,4303..5094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4452..4471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5265..5853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5754..5773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcaagct ttcctttagg aacagaggct tcgagccttt
      181 aaggctgcgt acttgcttct cctaatacca gagactcaaa aaaaaaaaaa aagttccaga
      241 ttgctggaca atgacccggg tctcatccct tgaccctggg aaccgggtcc acattgaatc
      301 aggtgcgaat gttcgctcgc cttctctgcc tttcccgcct cccctccccc ggccgcggcc
      361 ccggttcccc ccctgcgctg caccctcaga gttggctgca gccggcgagc tgttcccgtc
      421 aatccctccc tcctttacac aggatgtcca tattaggaca tctgcgtcag caggtttcca
      481 cggccggtcc ctgttgttct ggggggggga ccatctccga aatcctacac gcggaaggtc
      541 taggagaccc cctaagatcc caaatgtgaa cactcatagg tgaaagatgt atgccaagac
      601 gggggttgaa agcctggggc gtagagttga cgacagagcg cccgcagagg gccttggggc
      661 gcgcttcccc ccccttccag ttccgcccag tgacgtagga agtccatcca ttcacagcgc
      721 ttctataaag gcgccagctg aggcgcctac tactccaacc gcgactgcag cgagcaactg
      781 agaagactgg atagagccgg cggttccgcg aacgagcagt gaccgcgctc ccacccagct
      841 ctgctctgca gctcccacca gtgtctaccc ctggacccct tgccgggctt tccccaaact
      901 tcgaccatga tgttctcggg tttcaacgcc gactacgagg cgtcatcctc ccgctgcagt
      961 agcgcctccc cggccgggga cagcctttcc tactaccatt ccccagccga ctccttctcc
     1021 agcatgggct ctcctgtcaa cacacaggtg agtttggctt tgtgtagccg ccaggtccgc
     1081 gctgagggtc gccgtggagg agacactggg gtgtgactcg caggggcggg ggggtcttcc
     1141 tttttcgctc tggagggaga ctggcgcggt cagagcagcc ttagcctggg aacccaggac
     1201 ttgtctgagc gcgtgcacac ttgtcatagt aagacttagt gaccccttcc cgcgcggcag
     1261 gtttattctg agtggcctgc ctgcattctt ctctcggccg acttgtttct gagatcagcc
     1321 ggggccaaca agtctcgagc aaagagtcgc taactagagt ttgggaggcg gcaaaccgcg
     1381 gcaatccccc ctcccggggc agcctggagc agggaggagg gaggagggag gagggtgctg
     1441 cgggcgggtg tgtaaggcag tttcattgat aaaaagcgag ttcattctgg agactccgga
     1501 gcagcgcctg cgtcagcgca gacgtcaggg atatttataa caaaccccct ttcgagcgag
     1561 tgatgccgaa gggataacgg gaacgcagca gtaggatgga ggagaaaggc tgcgctgcgg
     1621 aattcaaggg aggatattgg gagagctttt atctccgatg aggtgcatac aggaagacat
     1681 aagcagtctc tgaccggaat gcttctctct ccctgcttca tgcgacacta gggccacttg
     1741 ctccacctgt gtctggaacc tcctcgctca cctccgcttt cctctttttg ttttgtttca
     1801 ggacttttgc gcagatctgg ccaccatgtc tagactggac aagagcaaag tgatcaacag
     1861 cgccctggaa ctgctgaacg gcgtgggcat cgagggcctg accacccgga agctggccca
     1921 gaaactgggc gtggaacagc ccaccctgta ctggcacgtg aagaacaagc gggccctgct
     1981 ggacgccctg cccatcgaga tgctggaccg gcaccacacc cacagctgcc ctctggaagg
     2041 cgagagctgg caggacttcc tgcggaacaa cgccaagagc tacagatgcg ccctgctgag
     2101 ccaccgggac ggcgccaaag tgcacctggg caccagaccc accgagaagc agtacgagac
     2161 actggaaaac cagctggcct tcctgtgcca gcagggcttc agcctggaaa acgccctgta
     2221 cgccctgagc gccgtgggcc acttcaccct gggctgcgtg ctggaagaac aggaacacca
     2281 ggtcgccaaa gaggaaagag agacacccac caccgacagc atgccccccc tgctgaagca
     2341 ggccatcgag ctgttcgaca gacagggcgc cgagcccgcc ttcctgttcg gcctggaact
     2401 gatcatctgc ggcctcgaga agcagctgaa gtgcgagagc ggcggaccca ccgacgccct
     2461 ggacgacttc gacctggaca tgctgcccgc cgatgccctg gatgattttg atctggatat
     2521 gctccctgct gacgctctcg atgacttcga tctcgatatg ctgccaggct aatcgataaa
     2581 acgttttatt gtgtttttaa tttatttatt aagatggatt ctcagatatt tatattttta
     2641 ttttattttt ttggatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2701 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2761 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2821 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     2881 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     2941 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3001 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3061 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3121 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3181 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3241 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3301 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3361 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     3481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3601 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3721 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     3781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     3841 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3961 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4021 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4081 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5881 ttcctggcct tttgctggcc ttttgctcac atgt
//
