LOCUS       sequence-23336-e         399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for C-terminal 3x HA tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE2c
FEATURES             Location/Qualifiers
     source          1..399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..187
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(256..282)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(256..282)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(283..309)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(283..309)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
       61 acacgggcca gagctgcagc tggatggcaa ataatgattt tattttgact gatagtgacc
      121 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
      181 gctgggtcta gatatctcga gagtactgct ctaggcttag tcgggcacgt cgtaggggta
      241 cggtccggat cgatcggcgt agtcgggcac gtcgtagggg taggcgtagt cgggcacgtc
      301 gtaggggtat cgcgacaggc ctccgcttgc ggccgcatgt ccgaaatctg taccggatcc
      361 agtcgactga attggttcct ttaaagcctg cttttttgt
//
