LOCUS       sequence-233429-        9507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjBCCIP
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3708..3752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3988..4212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4201..4218
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4569..6221)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     promoter        complement(6228..6979)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     promoter        6991..7490
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7026..7049)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7423..7442
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7511..8110
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7511..7530)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8007..8027
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8248..8481
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8553..8674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8590..8609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8644..8663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8693..8828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8860..8879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8861..8879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8889..8906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8889..8905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8898..8920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9050..9505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9137..9156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9347..9368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccaaaa gaaaaggggg gaggggatac cccctagagc
     3961 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     4021 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     4081 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     4141 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     4201 tagaaggcac agtcgaggct gatcagcgag ctctagcgtc gactctagat taaaattttt
     4261 aaaatgaaaa caaggtatct caaactccaa acgacagaca ctgcaacttc agaaaatcat
     4321 agcatttaag atccgaaggg aaactaccaa atgttgatta acttacggtt actaagtgct
     4381 gagatgtttt atccctccat gagactgtga gcctcctaaa tatccctgcc ttactacgac
     4441 agagcaattc caacagccat gatggttccc tgggaaccac ttagatttga tcttacttga
     4501 attagtccag ccttcagaga agccagggct gtcccttgcc ccggtgaacc acgtttaaac
     4561 aactagaatt acacggcgat cttgccgccc ttcttggcct taatgagaat ctcgcggatc
     4621 ttgcgggcgt ccaacttgcc ggtcagtcct ttaggcacct cgtccacgaa cacaacacca
     4681 ccgcgcagct tcttggcggt tgtaacctgg ctggccacat agtccacgat ctccttctcg
     4741 gtcatggttt taccgtgttc cagcacgacg actgcggcgg gcagctcgcc ggcatcgtcg
     4801 tcgggcaggc cggcgacccc ggcgtcgaag atgttggggt gttgcagcag gatgctctcc
     4861 agttcggctg gggctacctg gtagcccttg tatttgatca ggctcttcag ccggtccacg
     4921 atgaagaagt gctcgtcctc gtcccagtag gcgatgtcgc cgctgtgcag ccagccgtcc
     4981 ttgtcgatga gagcgtttgt agcctcgggg ttgttaacgt agccgctcat gatcatgggg
     5041 ccacggacgc acagctcgcc gcgctggttc acacccagtg tcttaccggt gtccaagtcc
     5101 accaccttag cctcgaagaa gggcaccacc ttgcctactg cgccaggctt gtcgtcccct
     5161 tcgggggtga tcagaatggc gctggttgtt tctgtcaggc cgtagccctg gcggatgcct
     5221 ggtaggtgga agcgtttggc cacggcctca cctacctcct tgctgagcgg cgccccgccg
     5281 ctggcgatct cgtgcaagtt gcttaggtcg tacttgtcga tgagagtgct cttagcgaag
     5341 aagctaaata gtgtgggcac cagcagggca gattgaatct tatagtcttg caagctgcgc
     5401 aagaatagct cctcctcgaa gcggtacatg agcacgaccc gaaagccgca gatcaagtag
     5461 cccagcgtgg tgaacatgcc gaagccgtgg tgaaatggca ccacgctgag gatagcggtg
     5521 tcggggatga tctggttgcc gaagatgggg tcgcgggcat gactgaatcg gacacaagcg
     5581 gtgcggtgcg gtagggctac gcccttgggc aatccggtac tgccactact gttcatgatc
     5641 agggcgatgg ttttgtcccg gtcgaagctc tcgggcacga agtcgtactc gttgaagccg
     5701 ggtggcaaat gggaagtcac gaaggtgtac atgctttgga agccctggta gtcggtcttg
     5761 ctatccatga tgatgatctt ttgtatgatc ggtagcttct tttgcacgtt gaggatcttt
     5821 tgcagccctt tcttgctcac gaatacgacg gtgggctggc tgatgcccat gctgttcagc
     5881 agctcgcgct cgttgtagat gtcgttagct ggggccacag ccacaccgat gaacagggca
     5941 cccaacacgg gcatgaagaa ctgcaagcta ttctcgctgc acaccacgat ccgatggttt
     6001 gtattcagcc catagcgctt catagcttct gccagccgaa cgctcatctc gaagtactcg
     6061 gcgtaggtaa tgtccacctc gatatgtgcg tcggtaaagg cgatggtgcc gggcaccagg
     6121 gcgtagcgct tcatggcttt gtgcagctgc tcgccggcgg tcccgtcttc gagtgggtag
     6181 aatggcgctg ggcccttctt aatgtttttg gcatcttcca tggatcctta agcgggtcgc
     6241 tgcagggtcg ctcggtgttc gaggccacac gcgtcacctt aatatgcgaa gtggacctgg
     6301 gaccgcgccg ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg
     6361 gggtttgtgt catcatagaa ctaaagacat gcaaatatat ttcttccggg gacaccgcca
     6421 gcaaacgcga gcaacgggcc acggggatga agcagctgcg ccactccctg aagctcctgc
     6481 agtccctcgc gcctccgggt gacaagatag tgtacctgtg ccccgtcctg gtgtttgtcg
     6541 cccaacggac gctccgcgtc agccgcgtga cccggctcgt cccgcagaag gtctccggta
     6601 atatcaccgc agtcgtgcgg atgctccaga gcctgtccac gtatacggtc cccatggagc
     6661 ctaggaccca gcgagcccgt cgccgccgcg gcggcgccgc ccgggggtct gcgagcagac
     6721 cgaaaaggtc acactctggg gcgcgcgacc cgcccgagtc agcggcccgc cagttaccac
     6781 ccgccgacca aacccccgcc tccacggagg gcgggggggt gcttaagagg atcgcggcgc
     6841 tcttctgcgt gcccgtggcc accaagacca aaccccgagc cgcctccgaa tgagagtgtt
     6901 tcgttccttc cccctccccc cgcgtcagac aaaccctaac caccgcttaa gcggcccccg
     6961 cgaggtccga agactcattt caattctacc gggtagggga ggcgcttttc ccaaggcagt
     7021 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     7081 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     7141 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     7201 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     7261 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     7321 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     7381 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     7441 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     7501 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7561 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7621 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7681 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7741 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7801 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7861 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7921 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7981 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     8041 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     8101 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     8161 tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
     8221 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
     8281 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8341 ctggctgcct agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8401 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8461 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8521 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8821 tggaggccta ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac
     8881 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8941 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9001 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt
     9061 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9121 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9181 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9241 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9301 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9361 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9421 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9481 aacaaaatat taacgtttac aatttcc
//
