LOCUS       sequence-233431-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjMGA
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3708..3752
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3988..4212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4201..4218
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4635..6287)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     promoter        complement(6294..7045)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     promoter        7057..7556
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7092..7115)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7489..7508
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7577..8176
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7577..7596)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8073..8093
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8314..8547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8619..8740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8656..8675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8710..8729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8759..8894
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8926..8945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8927..8945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8955..8972)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8955..8971)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8964..8986)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9116..9571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9203..9222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9413..9434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccaaaa gaaaaggggg gaggggatac cccctagagc
     3961 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     4021 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     4081 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     4141 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     4201 tagaaggcac agtcgaggct gatcagcgag ctctagcgtc gactctagaa ctgctttaat
     4261 tcttgtaact gatttatttc aggtttatat attctacctt tcaatcacca aacttctgtt
     4321 cttaggtttc taaaaaaata gtcacttttc gaaagcagaa aaggaaagat atatatattt
     4381 aatatataaa agaaaaatgc taaatctgac attatattct taaaaacatt ccatttattt
     4441 acagatgtat aaaaaggcga attattggtc taccgagatc atgcttctta tggtattagg
     4501 gtgagagctc actgtcctcc aggttcctca actcctctca ctcaacttca cctttcataa
     4561 tcacacatgt aatattcata gatcagtttc ccctagggta aggttaatgg gtagctagcg
     4621 ctaaacaact agaattacac ggcgatcttg ccgcccttct tggccttaat gagaatctcg
     4681 cggatcttgc gggcgtccaa cttgccggtc agtcctttag gcacctcgtc cacgaacaca
     4741 acaccaccgc gcagcttctt ggcggttgta acctggctgg ccacatagtc cacgatctcc
     4801 ttctcggtca tggttttacc gtgttccagc acgacgactg cggcgggcag ctcgccggca
     4861 tcgtcgtcgg gcaggccggc gaccccggcg tcgaagatgt tggggtgttg cagcaggatg
     4921 ctctccagtt cggctggggc tacctggtag cccttgtatt tgatcaggct cttcagccgg
     4981 tccacgatga agaagtgctc gtcctcgtcc cagtaggcga tgtcgccgct gtgcagccag
     5041 ccgtccttgt cgatgagagc gtttgtagcc tcggggttgt taacgtagcc gctcatgatc
     5101 atggggccac ggacgcacag ctcgccgcgc tggttcacac ccagtgtctt accggtgtcc
     5161 aagtccacca ccttagcctc gaagaagggc accaccttgc ctactgcgcc aggcttgtcg
     5221 tccccttcgg gggtgatcag aatggcgctg gttgtttctg tcaggccgta gccctggcgg
     5281 atgcctggta ggtggaagcg tttggccacg gcctcaccta cctccttgct gagcggcgcc
     5341 ccgccgctgg cgatctcgtg caagttgctt aggtcgtact tgtcgatgag agtgctctta
     5401 gcgaagaagc taaatagtgt gggcaccagc agggcagatt gaatcttata gtcttgcaag
     5461 ctgcgcaaga atagctcctc ctcgaagcgg tacatgagca cgacccgaaa gccgcagatc
     5521 aagtagccca gcgtggtgaa catgccgaag ccgtggtgaa atggcaccac gctgaggata
     5581 gcggtgtcgg ggatgatctg gttgccgaag atggggtcgc gggcatgact gaatcggaca
     5641 caagcggtgc ggtgcggtag ggctacgccc ttgggcaatc cggtactgcc actactgttc
     5701 atgatcaggg cgatggtttt gtcccggtcg aagctctcgg gcacgaagtc gtactcgttg
     5761 aagccgggtg gcaaatggga agtcacgaag gtgtacatgc tttggaagcc ctggtagtcg
     5821 gtcttgctat ccatgatgat gatcttttgt atgatcggta gcttcttttg cacgttgagg
     5881 atcttttgca gccctttctt gctcacgaat acgacggtgg gctggctgat gcccatgctg
     5941 ttcagcagct cgcgctcgtt gtagatgtcg ttagctgggg ccacagccac accgatgaac
     6001 agggcaccca acacgggcat gaagaactgc aagctattct cgctgcacac cacgatccga
     6061 tggtttgtat tcagcccata gcgcttcata gcttctgcca gccgaacgct catctcgaag
     6121 tactcggcgt aggtaatgtc cacctcgata tgtgcgtcgg taaaggcgat ggtgccgggc
     6181 accagggcgt agcgcttcat ggctttgtgc agctgctcgc cggcggtccc gtcttcgagt
     6241 gggtagaatg gcgctgggcc cttcttaatg tttttggcat cttccatgga tccttaagcg
     6301 ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg
     6361 acctgggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc
     6421 tgggcggggt ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca
     6481 ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca gctgcgccac tccctgaagc
     6541 tcctgcagtc cctcgcgcct ccgggtgaca agatagtgta cctgtgcccc gtcctggtgt
     6601 ttgtcgccca acggacgctc cgcgtcagcc gcgtgacccg gctcgtcccg cagaaggtct
     6661 ccggtaatat caccgcagtc gtgcggatgc tccagagcct gtccacgtat acggtcccca
     6721 tggagcctag gacccagcga gcccgtcgcc gccgcggcgg cgccgcccgg gggtctgcga
     6781 gcagaccgaa aaggtcacac tctggggcgc gcgacccgcc cgagtcagcg gcccgccagt
     6841 taccacccgc cgaccaaacc cccgcctcca cggagggcgg gggggtgctt aagaggatcg
     6901 cggcgctctt ctgcgtgccc gtggccacca agaccaaacc ccgagccgcc tccgaatgag
     6961 agtgtttcgt tccttccccc tccccccgcg tcagacaaac cctaaccacc gcttaagcgg
     7021 cccccgcgag gtccgaagac tcatttcaat tctaccgggt aggggaggcg cttttcccaa
     7081 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     7141 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     7201 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     7261 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     7321 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     7381 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     7441 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     7501 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     7561 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7621 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7681 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7741 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7801 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7861 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7921 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7981 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8041 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     8101 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8161 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     8221 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     8281 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     8341 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8401 agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8461 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8521 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     8581 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8701 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8761 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8821 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8881 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
     8941 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9001 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9061 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
     9121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9481 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9541 aattttaaca aaatattaac gtttacaatt tcc
//
