LOCUS       sequence-233522-       12448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi plasmid for use in Candida albicans. Contains dCas9 and 
            NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, 
            PacI sites, sgRNA tail).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-02-13"; volume: "4(1). pii: 4/1/e00002-19. doi"; pages: 
            " 10.1128/mSphere.00002-19"
COMMENT     Sequence Label: pRS143
FEATURES             Location/Qualifiers
     source          1..12448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(3237..3263,3264..3266,3267..5822,5823..5825,
                     5826..7340)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7353..7373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     7378..7394
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7379..7395
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8335..8532)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(9133..9476)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(10430..10449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10431..10449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10456..10473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10456..10472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10465..10487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10701..10720)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(10757..10831)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     12290..12311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgta agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgaattgg agggtaccag
     2221 agctattaag atcaccagcc tcgtttataa taaacttagt ctaatatttc ttttggacca
     2281 aaatagaact gttctcaggt ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc
     2341 ggctatgcca atcaaaaagg agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg
     2401 cgaaacaaca attttaatgc ttactgtgcc gccaccaata tcctatggcc aaggggcctt
     2461 taaacatgcc acggaaatat acagtcaaag taaacctact atgtctttag agccttcagg
     2521 atcaattgtt tttaaatgaa tgactgcaaa actagaaaag tagcctctta cataagcaag
     2581 tcatttgtac accgagagtt tagtaaccag tgaagcaagt atatctggca aacctgaaac
     2641 ttatctgtga attataaagc tcataagtaa aaagtgtaga aaagaagata aggcacacca
     2701 aatacaaatt tgcaacagta caacgaatat ttcagcgtca aaactagaga ataataaaga
     2761 aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg
     2821 tgctatttat attatatagt ttactcgtaa taccataaaa tattcggttt gattaggtta
     2881 ttttaataag ctaatttgtt ttctaatcgt gtaatttatg ctgtgtatat taagtagtgt
     2941 gtgtgcactg cccaaaaatg tttgttgttt atagtcggtt aaagagaaaa aagaaaaaaa
     3001 gatccataca cacacgttaa ttagttgttc aacgtaatac acttatattt tgttcttatt
     3061 tgctttcggt cgctgttctc accaggattt attgccaacg aaacaatttt tttttatata
     3121 ttttcagatt tttctttttt tcctttcctt tccttttcta attttcactc ctggttttct
     3181 ttctttctta gaaacattat ctcgatatta atattaaaaa aatataatca ttcaaaatgg
     3241 ataaaaaata ctccataggt ttggccattg gaaccaattc agttggatgg gctgtaatca
     3301 ctgatgaata taaggttcct tctaaaaagt tcaaagtttt aggaaatact gatagacact
     3361 ccattaaaaa gaatttaatt ggtgccttgt tgtttgacag tggtgaaaca gctgaagcaa
     3421 ctcgtttgaa aagaacagct agaagaagat ataccagaag aaagaacaga atttgttact
     3481 tgcaagaaat cttttctaat gaaatggcaa aggttgatga ttcttttttt catagattgg
     3541 aagaaagttt cttagttgaa gaagataaga aacatgaaag acatccaatc tttggtaata
     3601 ttgtagatga agtggcttac cacgaaaaat atcctacaat ttatcatttg agaaaaaaat
     3661 tagttgactc cactgataaa gctgacttaa gacttatata tttggcattg gctcacatga
     3721 tcaagttcag aggtcacttc ttaattgaag gtgatttgaa tccagataat tccgatgtcg
     3781 acaagttgtt tattcaattg gttcaaactt acaaccaact tttcgaggaa aatccaatca
     3841 atgcctcagg tgtggatgct aaagctattt tatctgctag attgtcaaaa agtagacgtt
     3901 tggaaaatct tattgcccaa ttgccaggtg aaaaaaaaaa tggattgttt ggaaacttaa
     3961 ttgcattatc tttgggtttg accccaaatt tcaaatcaaa ctttgatttg gccgaggatg
     4021 ctaaacttca attgtctaag gatacatacg atgacgattt agataatctt cttgcccaaa
     4081 ttggtgatca atatgcagat ttattcttgg cagctaagaa cttgagtgat gcaattttgt
     4141 taagtgacat tttgagagtc aacacagaaa ttacaaaagc tcctttatcc gcctctatga
     4201 ttaaaagata tgatgaacac catcaagatt taaccttgtt gaaagctctt gtacgtcaac
     4261 aattaccaga aaagtataaa gaaatattct ttgatcaatc taaaaatggt tacgctggtt
     4321 atattgatgg tggtgctagt caagaagaat tttataagtt tatcaagcca attcttgaaa
     4381 aaatggatgg tactgaggaa ttgttggtta agttgaatcg agaggattta cttcgtaagc
     4441 aaagaacatt tgataacggt tctatccctc atcaaatcca cttaggtgag ttacacgcta
     4501 tcttgagacg tcaggaagat ttttaccctt ttttgaaaga caatcgtgaa aaaattgaaa
     4561 aaattttgac tttcagaatt ccttattacg tcgggccatt ggctagaggt aattctagat
     4621 ttgcatggat gactcgtaaa tctgaagaga ctatcacccc atggaatttt gaggaagttg
     4681 ttgataaggg agcctccgct caatcattca tcgagagaat gaccaatttc gacaaaaact
     4741 tgccaaatga gaaggtgtta cctaaacatt ctttgttata tgaatatttc actgtttata
     4801 atgaattaac aaaagtcaaa tacgtcactg aaggtatgag aaaaccagca ttcttaagtg
     4861 gtgaacaaaa gaaagctatc gttgacttgt tattcaaaac taatcgtaaa gtcactgtca
     4921 aacaacttaa agaggactat tttaagaaaa tagagtgttt cgattccgtt gaaatcagtg
     4981 gggttgagga tagatttaat gctagtttag gaacttatca cgatttgttg aaaattatca
     5041 aagacaaaga ttttttggat aatgaagaaa acgaagatat cttggaggac attgtattga
     5101 ctttgacttt gtttgaggat agagaaatga tcgaagaacg tttaaaaact tacgctcact
     5161 tattcgacga taaagtcatg aaacaattaa aaagaagaag atataccggt tggggaagat
     5221 tatctagaaa gttgattaat ggtattagag ataagcaaag tggtaaaacc atcttagatt
     5281 tccttaaaag tgatggtttt gcaaacagaa actttatgca gttgattcat gatgatagtt
     5341 tgacctttaa ggaagatatt caaaaagctc aggtttctgg acagggtgat tcattgcatg
     5401 aacatattgc caacttagca gggtctccag ctatcaaaaa gggaatttta caaactgtta
     5461 aagttgttga tgagttggtg aaagttatgg gacgtcataa gcctgaaaat atagttatag
     5521 aaatggctag agaaaatcaa acaactcaga aaggacaaaa gaattctcgt gaaagaatga
     5581 aaagaataga agaaggaatt aaagagttgg gttctcaaat cttaaaagaa cacccagtcg
     5641 aaaacaccca gttacagaac gagaagcttt acttgtacta tttgcaaaac gggcgtgaca
     5701 tgtatgtaga tcaagagttg gatatcaata gactttcaga ttacgatgtc gatcacatag
     5761 ttcctcagtc atttttaaaa gatgattcca ttgacaacaa agttttaaca agatctgata
     5821 aggctagagg taaatcagac aatgtgccat cagaagaagt ggttaagaaa atgaagaact
     5881 attggagaca attgttaaat gcaaagttga ttactcagag aaaatttgac aacttaacta
     5941 aagctgaaag aggaggatta tccgaattag ataaagctgg attcataaaa cgtcagttgg
     6001 ttgaaacaag acaaattact aaacatgtag cccaaatttt agattcccga atgaatacta
     6061 aatatgatga aaatgacaaa ttaatcagag aggtcaaagt gatcacatta aagtcaaagt
     6121 tggtgtcaga tttccgaaag gattttcaat tctacaaggt gagagagatc aacaattatc
     6181 atcatgctca cgacgcatat ttgaatgcag tggtaggaac tgccttaata aagaagtatc
     6241 ctaagttaga atccgaattc gtgtatggtg attataaagt ctacgatgtc agaaagatga
     6301 tagcaaaatc agaacaagaa attgggaaag ccaccgcaaa gtatttcttt tactccaaca
     6361 tcatgaattt ctttaagacc gaaatcacct tggctaacgg ggaaatcaga aaacgtcctt
     6421 tgattgaaac aaatggagaa accggtgaaa ttgtgtggga taaggggaga gattttgcta
     6481 ctgttagaaa agttttatca atgcctcaag ttaatattgt aaagaagact gaagttcaaa
     6541 ctggtggttt ctctaaggaa tccattcttc caaaacgaaa ctcagacaag ttgatcgccc
     6601 gaaaaaaaga ttgggatcct aagaaatacg gaggttttga ttctcctact gtcgcatatt
     6661 ctgtcttggt cgtggctaaa gttgaaaagg gtaaatccaa aaaattgaaa tctgtgaaag
     6721 aattgttagg tattaccatc atggagcgtt catcctttga gaaaaaccca attgactttc
     6781 tcgaagcgaa gggctataaa gaagtgaaga aagatttgat tattaagttg ccaaagtatt
     6841 ctctctttga acttgaaaat gggagaaaga gaatgttggc ttcagctgga gaattgcaaa
     6901 aaggaaacga gttagctttg ccttcaaagt acgttaattt cttatactta gcatctcatt
     6961 atgaaaagtt gaaggggtcc cctgaagaca atgagcaaaa gcaattattc gtagaacaac
     7021 ataaacacta tttggatgag ataattgaac aaatctccga gttttctaaa agagttattt
     7081 tggctgatgc aaatttggat aaagttttat ctgcttataa taagcatcgt gacaaaccaa
     7141 ttcgagaaca agctgaaaat attatccact tgttcacatt gactaacttg ggagctccag
     7201 ccgcgtttaa gtactttgac accaccattg accgtaagag atatacttca acaaaagaag
     7261 tgttagacgc cacattgatt catcagtcca taactggttt atatgaaact agaatcgatt
     7321 tgtcacaatt ggggggtgat agccgagcgg atcctaagaa aaaacgaaaa gtctgactcg
     7381 aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccgagtgaa
     7441 attctggaaa tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat
     7501 atataacttg ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa
     7561 tttaagaaaa agagaaaaat gttttatttg agagattgat attttacttg aatttagctt
     7621 agcttttata aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga
     7681 ctataaaata gccacccaag gcatttctat atcttgttgt tgttgttttc atcttctgta
     7741 tcagaggaac ttattttatt attttcgtca cgggtatttt ctcttgtttg atgattcatc
     7801 ccattcattc catcataaaa ttctagaagg accacctttg attgtaaata gtaataatta
     7861 ccacccttat ttaattattt atttaactta tttatttatt tattctacat atatacaaat
     7921 ctaataaagt gaaaatctcc cccttcacat ttataattgg ccagtctttt tcaaataagc
     7981 attccaacca gcatttctat accttttacc ttcaatatct ggatctcttc ctttaccaaa
     8041 caatcctcta ccaacaatga taatatcagt tccagtgcta acaacttcat caacagttct
     8101 atattgttgt cctaatccat cacctttatc atctaatcca actccaggtg tcataataag
     8161 ccaatcaaat ccttcttctt ggccacccat atcacgttgg gcaataaatc caataacaaa
     8221 ttccttatcg gatttagcaa tttcaacagt tttttgagaa tattctccat agtaccgaat
     8281 tcgagctcgt ttaaacgtcg acactggatg gcggcgttag tatcgaatcg acagcagtat
     8341 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
     8401 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
     8461 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
     8521 tttattgtca gtactgactc gagttattat ggacatggca tagacatata caaagcttgt
     8581 tcaccatcgg aagcagtacc atcgtataaa gcagtatcca aaccacacaa agtgaaaccc
     8641 attcttctat aagcatgaat agctggagca ttaacattgg taacttccaa ccacaaatga
     8701 ccagcacctc tttctctggc gaattcagta gccaaaccca tcaaagctct accaacacca
     8761 tgacctctat gttctggagc aacttcaata tcttcaacag tcaatcttct gttccaacca
     8821 gaataagaaa caacaacgaa accagccaaa tcaccatcat caccataagc aacgaaagtt
     8881 ctagaatctg gatcaccatc ttcaccagca tcggattcat catcggattc atcatctggg
     8941 aaaaccttag tcaatggtgg atcaactgga acttctctca aagtgaaacc atcaccagta
     9001 gcagtaactc taaaaacagt atcggtagtg aaagaaccat ccaaagcttc aatagcttca
     9061 gcatcacctg gaacagaagt tctgtatcta taagcagtat catccaaagt agtagacata
     9121 attgtaggat ccggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt
     9181 ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc
     9241 tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg
     9301 caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg
     9361 gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat
     9421 gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg
     9481 gccgggtgac ccggcgggga cgaggcaagc ttgattgcta atgatcccac tgatgataat
     9541 tcagctaaca tcaataaccc tcttggctct tggttggtgg tggtttcttt agctccctgt
     9601 tttaatcctt caaccactcc attcccagtg acaccatgag cattggtaat atctgcccaa
     9661 ctactaattt tataaactcc accaatatat tgtttcttta cggtattacc aatatcagca
     9721 aattttctat cttcaaaaat cataaattga tgtttacgtg aaagttctaa taatggttca
     9781 atagtggatt cataggaaaa atcattgatt atatcaatat gagtcttgat taagcataca
     9841 taaggaccta atttatcaat taattcaagg aattccttag tggtatcaac gtcaattgat
     9901 gcacataaat tggttttctt cagttccatt aatcgaaata atcgctgtgc tactggtgag
     9961 gcatgagttt ctgctctctc actataggtc ttagtgttga ctgtcatatc ttttgtttgc
    10021 tatttctatt tctatttctt tcttcaactg taaaaggggc ggtatataaa ccagtgtgta
    10081 tggggttgtt gctcaggaat tgatcaatgt ttaaaagaat ggttgaaatc atcattgaag
    10141 aacaaaaaaa aagatttttt ctttcattat gttatctgta taatatgcag gaaataagat
    10201 tgcaaaaaaa tagccgtttt tttggatact atcaaacaag aggaatgact aatacagaat
    10261 tagtgttacg aatcaatggc actacagcaa ctttcaacaa aataatctac gttcaaaata
    10321 taatatatac tattaaataa aatcaaataa taaaataatt ttaaagaaag tagtaccagc
    10381 tctttttttt gtttccgttt ataccatcca aatcaattcc tattagtcgc cctatagtga
    10441 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    10501 tacccaactt aatcgccttg cagcacatcc ccccttcgcc agctggcgta atagcgaaga
    10561 ggcccgcacc gatcgccctt cccaacagtt gcgtagcctg aatggcgaat gggacgcgcc
    10621 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    10681 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    10741 cggcaaaaaa aagcaccacc gactcggtgc cactttttca agttgataac ggactagcct
    10801 tattttaact tgctatttct agctctaaaa ctgaagagcc attaatgctc ttcctcgaaa
    10861 ttaaaaatag tttacgcaag tctcgaactt ccaccacaat ttaattgtaa gaacgctgcg
    10921 aaaacatatt ctccaatcct attgggccga agtttcagct atgccaaagg catcaaattt
    10981 ttttttataa aattttccac ttttccatac cagtgaaatt atcacgtgat ggagtgagta
    11041 tctataatat ttgattgttt ctttgatgag gcaatactcg agtatagtta ttgaccaatg
    11101 acttttaccg gctaaacaag tggtattcaa gcacaattca gttttctgga tctctaataa
    11161 ttgcaattgc aattgcttca catactctgt ttataattca gtttctttct cttgatgtta
    11221 ctgatatatt aagttaataa ataccaataa ctttttttca aaaatcttct attttccttt
    11281 caattgatta tttaaacacg aatcgttaat aagctgtgat tgctttttct ttttcttttt
    11341 cttttctttg ttcaagtttg aaaaaagtaa aaaaaaaggt ttctcttgtt cgatgtagaa
    11401 ttcagtgggt tatagttgta atgatatatg gaataaaaag aaagaaagaa agagaagaga
    11461 aagagaagaa gagaagaatt aagtaaacgt gtattagaat ttaaaaatta aaattcatga
    11521 taatctttaa taataattaa ctaagaatca caaaattgaa aatttgaaag aaaaaagatc
    11581 actccaaggt cgtgtcaagt gactagaaat tcttaattaa aatagttata acaaaaaaag
    11641 aaatttgtat ataacaatta agtaaaagaa aaaaaagcaa ttcgctttgt ataataatac
    11701 ccagaaaaat atattcatat attcataaat caaacatgaa atgaagaatg ctgaatcact
    11761 tgataggatt tagttccatt atggtactaa gaagtaatat tacttctatg aaaaggttga
    11821 atccgagatc aaaattcatc ttttcatata ctctggagaa aaaaaaaaaa gtaattgtag
    11881 taagaatgac aagtatcagt tgagaaaaaa agtaacaaag agaaagctcg gaggaggctc
    11941 cccaaaggtt ttatcaccaa tggtggtacc actaacccaa gaacagaaaa agcattgata
    12001 ttttgaaaaa atacaatact catcttttaa cccgaacctt ttcttgtcct ttaaaacttt
    12061 tatctcatat ctgtccaaag tcatattgtc ttactacttt ctctccttca tcgtccttcc
    12121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    12181 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    12241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    12301 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    12361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    12421 tttaacaaaa tattaacgtt tacaattt
//
