LOCUS       sequence-233523-       12784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and
            NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, 
            PacI sites, sgRNA tail).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-02-13"; volume: "4(1). pii: 4/1/e00002-19. doi"; pages: 
            " 10.1128/mSphere.00002-19"
COMMENT     Sequence Label: pRS159
FEATURES             Location/Qualifiers
     source          1..12784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(3237..3263,3264..3266,3267..5822,5823..5825,
                     5826..7340)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7353..7373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(8671..8868)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(9469..9812)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(10766..10785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10767..10785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10792..10809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10792..10808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10801..10823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11037..11056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(11093..11167)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     12626..12647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgta agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgaattgg agggtaccag
     2221 agctattaag atcaccagcc tcgtttataa taaacttagt ctaatatttc ttttggacca
     2281 aaatagaact gttctcaggt ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc
     2341 ggctatgcca atcaaaaagg agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg
     2401 cgaaacaaca attttaatgc ttactgtgcc gccaccaata tcctatggcc aaggggcctt
     2461 taaacatgcc acggaaatat acagtcaaag taaacctact atgtctttag agccttcagg
     2521 atcaattgtt tttaaatgaa tgactgcaaa actagaaaag tagcctctta cataagcaag
     2581 tcatttgtac accgagagtt tagtaaccag tgaagcaagt atatctggca aacctgaaac
     2641 ttatctgtga attataaagc tcataagtaa aaagtgtaga aaagaagata aggcacacca
     2701 aatacaaatt tgcaacagta caacgaatat ttcagcgtca aaactagaga ataataaaga
     2761 aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg
     2821 tgctatttat attatatagt ttactcgtaa taccataaaa tattcggttt gattaggtta
     2881 ttttaataag ctaatttgtt ttctaatcgt gtaatttatg ctgtgtatat taagtagtgt
     2941 gtgtgcactg cccaaaaatg tttgttgttt atagtcggtt aaagagaaaa aagaaaaaaa
     3001 gatccataca cacacgttaa ttagttgttc aacgtaatac acttatattt tgttcttatt
     3061 tgctttcggt cgctgttctc accaggattt attgccaacg aaacaatttt tttttatata
     3121 ttttcagatt tttctttttt tcctttcctt tccttttcta attttcactc ctggttttct
     3181 ttctttctta gaaacattat ctcgatatta atattaaaaa aatataatca ttcaaaatgg
     3241 ataaaaaata ctccataggt ttggccattg gaaccaattc agttggatgg gctgtaatca
     3301 ctgatgaata taaggttcct tctaaaaagt tcaaagtttt aggaaatact gatagacact
     3361 ccattaaaaa gaatttaatt ggtgccttgt tgtttgacag tggtgaaaca gctgaagcaa
     3421 ctcgtttgaa aagaacagct agaagaagat ataccagaag aaagaacaga atttgttact
     3481 tgcaagaaat cttttctaat gaaatggcaa aggttgatga ttcttttttt catagattgg
     3541 aagaaagttt cttagttgaa gaagataaga aacatgaaag acatccaatc tttggtaata
     3601 ttgtagatga agtggcttac cacgaaaaat atcctacaat ttatcatttg agaaaaaaat
     3661 tagttgactc cactgataaa gctgacttaa gacttatata tttggcattg gctcacatga
     3721 tcaagttcag aggtcacttc ttaattgaag gtgatttgaa tccagataat tccgatgtcg
     3781 acaagttgtt tattcaattg gttcaaactt acaaccaact tttcgaggaa aatccaatca
     3841 atgcctcagg tgtggatgct aaagctattt tatctgctag attgtcaaaa agtagacgtt
     3901 tggaaaatct tattgcccaa ttgccaggtg aaaaaaaaaa tggattgttt ggaaacttaa
     3961 ttgcattatc tttgggtttg accccaaatt tcaaatcaaa ctttgatttg gccgaggatg
     4021 ctaaacttca attgtctaag gatacatacg atgacgattt agataatctt cttgcccaaa
     4081 ttggtgatca atatgcagat ttattcttgg cagctaagaa cttgagtgat gcaattttgt
     4141 taagtgacat tttgagagtc aacacagaaa ttacaaaagc tcctttatcc gcctctatga
     4201 ttaaaagata tgatgaacac catcaagatt taaccttgtt gaaagctctt gtacgtcaac
     4261 aattaccaga aaagtataaa gaaatattct ttgatcaatc taaaaatggt tacgctggtt
     4321 atattgatgg tggtgctagt caagaagaat tttataagtt tatcaagcca attcttgaaa
     4381 aaatggatgg tactgaggaa ttgttggtta agttgaatcg agaggattta cttcgtaagc
     4441 aaagaacatt tgataacggt tctatccctc atcaaatcca cttaggtgag ttacacgcta
     4501 tcttgagacg tcaggaagat ttttaccctt ttttgaaaga caatcgtgaa aaaattgaaa
     4561 aaattttgac tttcagaatt ccttattacg tcgggccatt ggctagaggt aattctagat
     4621 ttgcatggat gactcgtaaa tctgaagaga ctatcacccc atggaatttt gaggaagttg
     4681 ttgataaggg agcctccgct caatcattca tcgagagaat gaccaatttc gacaaaaact
     4741 tgccaaatga gaaggtgtta cctaaacatt ctttgttata tgaatatttc actgtttata
     4801 atgaattaac aaaagtcaaa tacgtcactg aaggtatgag aaaaccagca ttcttaagtg
     4861 gtgaacaaaa gaaagctatc gttgacttgt tattcaaaac taatcgtaaa gtcactgtca
     4921 aacaacttaa agaggactat tttaagaaaa tagagtgttt cgattccgtt gaaatcagtg
     4981 gggttgagga tagatttaat gctagtttag gaacttatca cgatttgttg aaaattatca
     5041 aagacaaaga ttttttggat aatgaagaaa acgaagatat cttggaggac attgtattga
     5101 ctttgacttt gtttgaggat agagaaatga tcgaagaacg tttaaaaact tacgctcact
     5161 tattcgacga taaagtcatg aaacaattaa aaagaagaag atataccggt tggggaagat
     5221 tatctagaaa gttgattaat ggtattagag ataagcaaag tggtaaaacc atcttagatt
     5281 tccttaaaag tgatggtttt gcaaacagaa actttatgca gttgattcat gatgatagtt
     5341 tgacctttaa ggaagatatt caaaaagctc aggtttctgg acagggtgat tcattgcatg
     5401 aacatattgc caacttagca gggtctccag ctatcaaaaa gggaatttta caaactgtta
     5461 aagttgttga tgagttggtg aaagttatgg gacgtcataa gcctgaaaat atagttatag
     5521 aaatggctag agaaaatcaa acaactcaga aaggacaaaa gaattctcgt gaaagaatga
     5581 aaagaataga agaaggaatt aaagagttgg gttctcaaat cttaaaagaa cacccagtcg
     5641 aaaacaccca gttacagaac gagaagcttt acttgtacta tttgcaaaac gggcgtgaca
     5701 tgtatgtaga tcaagagttg gatatcaata gactttcaga ttacgatgtc gatcacatag
     5761 ttcctcagtc atttttaaaa gatgattcca ttgacaacaa agttttaaca agatctgata
     5821 aggctagagg taaatcagac aatgtgccat cagaagaagt ggttaagaaa atgaagaact
     5881 attggagaca attgttaaat gcaaagttga ttactcagag aaaatttgac aacttaacta
     5941 aagctgaaag aggaggatta tccgaattag ataaagctgg attcataaaa cgtcagttgg
     6001 ttgaaacaag acaaattact aaacatgtag cccaaatttt agattcccga atgaatacta
     6061 aatatgatga aaatgacaaa ttaatcagag aggtcaaagt gatcacatta aagtcaaagt
     6121 tggtgtcaga tttccgaaag gattttcaat tctacaaggt gagagagatc aacaattatc
     6181 atcatgctca cgacgcatat ttgaatgcag tggtaggaac tgccttaata aagaagtatc
     6241 ctaagttaga atccgaattc gtgtatggtg attataaagt ctacgatgtc agaaagatga
     6301 tagcaaaatc agaacaagaa attgggaaag ccaccgcaaa gtatttcttt tactccaaca
     6361 tcatgaattt ctttaagacc gaaatcacct tggctaacgg ggaaatcaga aaacgtcctt
     6421 tgattgaaac aaatggagaa accggtgaaa ttgtgtggga taaggggaga gattttgcta
     6481 ctgttagaaa agttttatca atgcctcaag ttaatattgt aaagaagact gaagttcaaa
     6541 ctggtggttt ctctaaggaa tccattcttc caaaacgaaa ctcagacaag ttgatcgccc
     6601 gaaaaaaaga ttgggatcct aagaaatacg gaggttttga ttctcctact gtcgcatatt
     6661 ctgtcttggt cgtggctaaa gttgaaaagg gtaaatccaa aaaattgaaa tctgtgaaag
     6721 aattgttagg tattaccatc atggagcgtt catcctttga gaaaaaccca attgactttc
     6781 tcgaagcgaa gggctataaa gaagtgaaga aagatttgat tattaagttg ccaaagtatt
     6841 ctctctttga acttgaaaat gggagaaaga gaatgttggc ttcagctgga gaattgcaaa
     6901 aaggaaacga gttagctttg ccttcaaagt acgttaattt cttatactta gcatctcatt
     6961 atgaaaagtt gaaggggtcc cctgaagaca atgagcaaaa gcaattattc gtagaacaac
     7021 ataaacacta tttggatgag ataattgaac aaatctccga gttttctaaa agagttattt
     7081 tggctgatgc aaatttggat aaagttttat ctgcttataa taagcatcgt gacaaaccaa
     7141 ttcgagaaca agctgaaaat attatccact tgttcacatt gactaacttg ggagctccag
     7201 ccgcgtttaa gtactttgac accaccattg accgtaagag atatacttca acaaaagaag
     7261 tgttagacgc cacattgatt catcagtcca taactggttt atatgaaact agaatcgatt
     7321 tgtcacaatt ggggggtgat agccgagcgg atcctaagaa aaaacgaaaa gtcagtggtg
     7381 gtggtagtgg gtccgggtca ggaggttcat ccaagttagg aggttcagga gggagtatgg
     7441 aacgagttag aatgatcaac gtacagcgtt tgttagaggc tgcagagttt ttggaaagac
     7501 gagaacgaga atgcgagcat ggttacgcat cttccttccc atctatgcca tccccacgag
     7561 ggagtggagg ggggtctggg tccggttctg gatctgggag ttcaaaatta gggggatccg
     7621 ggggatctat ggaacgtgtc cgtatgatta atgtccaaag attattagaa gccgcagaat
     7681 ttttagaaag acgagaaaga gaatgtgaac atggttatgc atcatcattt ccaagtatgc
     7741 cttcaccaag aggttaaccc gggggatccg agtgaaattc tggaaatctg gaaatctggt
     7801 tttgtattct tgttattctt ctttttgtta ttacatatat aacttgttac ttttttaaaa
     7861 aaatctttgt ttattttata aatatataaa actaaattta agaaaaagag aaaaatgttt
     7921 tatttgagag attgatattt tacttgaatt tagcttagct tttataaagt attattatgt
     7981 aaaaaaacaa aacaaatata cattaaaaag ttaagactat aaaatagcca cccaaggcat
     8041 ttctatatct tgttgttgtt gttttcatct tctgtatcag aggaacttat tttattattt
     8101 tcgtcacggg tattttctct tgtttgatga ttcatcccat tcattccatc ataaaattct
     8161 agaaggacca cctttgattg taaatagtaa taattaccac ccttatttaa ttatttattt
     8221 aacttattta tttatttatt ctacatatat acaaatctaa taaagtgaaa atctccccct
     8281 tcacatttat aattggccag tctttttcaa ataagcattc caaccagcat ttctatacct
     8341 tttaccttca atatctggat ctcttccttt accaaacaat cctctaccaa caatgataat
     8401 atcagttcca gtgctaacaa cttcatcaac agttctatat tgttgtccta atccatcacc
     8461 tttatcatct aatccaactc caggtgtcat aataagccaa tcaaatcctt cttcttggcc
     8521 acccatatca cgttgggcaa taaatccaat aacaaattcc ttatcggatt tagcaatttc
     8581 aacagttttt tgagaatatt ctccatagta ccgaattcga gctcgtttaa acgtcgacac
     8641 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
     8701 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
     8761 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
     8821 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgactcgagt
     8881 tattatggac atggcataga catatacaaa gcttgttcac catcggaagc agtaccatcg
     8941 tataaagcag tatccaaacc acacaaagtg aaacccattc ttctataagc atgaatagct
     9001 ggagcattaa cattggtaac ttccaaccac aaatgaccag cacctctttc tctggcgaat
     9061 tcagtagcca aacccatcaa agctctacca acaccatgac ctctatgttc tggagcaact
     9121 tcaatatctt caacagtcaa tcttctgttc caaccagaat aagaaacaac aacgaaacca
     9181 gccaaatcac catcatcacc ataagcaacg aaagttctag aatctggatc accatcttca
     9241 ccagcatcgg attcatcatc ggattcatca tctgggaaaa ccttagtcaa tggtggatca
     9301 actggaactt ctctcaaagt gaaaccatca ccagtagcag taactctaaa aacagtatcg
     9361 gtagtgaaag aaccatccaa agcttcaata gcttcagcat cacctggaac agaagttctg
     9421 tatctataag cagtatcatc caaagtagta gacataattg taggatccgg ttgtttatgt
     9481 tcggatgtga tgtgagaact gtatcctagc aagattttaa aaggaagtat atgaaagaag
     9541 aacctcagtg gcaaatccta accttttata tttctctaca ggggcgcggc gtggggacaa
     9601 ttcaacgcgt ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa
     9661 tttttgcttc gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga
     9721 tgtatgggct aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga
     9781 ctgtcaagga gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag
     9841 gcaagcttga ttgctaatga tcccactgat gataattcag ctaacatcaa taaccctctt
     9901 ggctcttggt tggtggtggt ttctttagct ccctgtttta atccttcaac cactccattc
     9961 ccagtgacac catgagcatt ggtaatatct gcccaactac taattttata aactccacca
    10021 atatattgtt tctttacggt attaccaata tcagcaaatt ttctatcttc aaaaatcata
    10081 aattgatgtt tacgtgaaag ttctaataat ggttcaatag tggattcata ggaaaaatca
    10141 ttgattatat caatatgagt cttgattaag catacataag gacctaattt atcaattaat
    10201 tcaaggaatt ccttagtggt atcaacgtca attgatgcac ataaattggt tttcttcagt
    10261 tccattaatc gaaataatcg ctgtgctact ggtgaggcat gagtttctgc tctctcacta
    10321 taggtcttag tgttgactgt catatctttt gtttgctatt tctatttcta tttctttctt
    10381 caactgtaaa aggggcggta tataaaccag tgtgtatggg gttgttgctc aggaattgat
    10441 caatgtttaa aagaatggtt gaaatcatca ttgaagaaca aaaaaaaaga ttttttcttt
    10501 cattatgtta tctgtataat atgcaggaaa taagattgca aaaaaatagc cgtttttttg
    10561 gatactatca aacaagagga atgactaata cagaattagt gttacgaatc aatggcacta
    10621 cagcaacttt caacaaaata atctacgttc aaaatataat atatactatt aaataaaatc
    10681 aaataataaa ataattttaa agaaagtagt accagctctt ttttttgttt ccgtttatac
    10741 catccaaatc aattcctatt agtcgcccta tagtgagtcg tattacaatt cactggccgt
    10801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10861 acatcccccc ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10921 acagttgcgt agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
    10981 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    11041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc aaaaaaaagc accaccgact
    11101 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
    11161 ctaaaactga agagccatta atgctcttcc tcgaaattaa aaatagttta cgcaagtctc
    11221 gaacttccac cacaatttaa ttgtaagaac gctgcgaaaa catattctcc aatcctattg
    11281 ggccgaagtt tcagctatgc caaaggcatc aaattttttt ttataaaatt ttccactttt
    11341 ccataccagt gaaattatca cgtgatggag tgagtatcta taatatttga ttgtttcttt
    11401 gatgaggcaa tactcgagta tagttattga ccaatgactt ttaccggcta aacaagtggt
    11461 attcaagcac aattcagttt tctggatctc taataattgc aattgcaatt gcttcacata
    11521 ctctgtttat aattcagttt ctttctcttg atgttactga tatattaagt taataaatac
    11581 caataacttt ttttcaaaaa tcttctattt tcctttcaat tgattattta aacacgaatc
    11641 gttaataagc tgtgattgct ttttcttttt ctttttcttt tctttgttca agtttgaaaa
    11701 aagtaaaaaa aaaggtttct cttgttcgat gtagaattca gtgggttata gttgtaatga
    11761 tatatggaat aaaaagaaag aaagaaagag aagagaaaga gaagaagaga agaattaagt
    11821 aaacgtgtat tagaatttaa aaattaaaat tcatgataat ctttaataat aattaactaa
    11881 gaatcacaaa attgaaaatt tgaaagaaaa aagatcactc caaggtcgtg tcaagtgact
    11941 agaaattctt aattaaaata gttataacaa aaaaagaaat ttgtatataa caattaagta
    12001 aaagaaaaaa aagcaattcg ctttgtataa taatacccag aaaaatatat tcatatattc
    12061 ataaatcaaa catgaaatga agaatgctga atcacttgat aggatttagt tccattatgg
    12121 tactaagaag taatattact tctatgaaaa ggttgaatcc gagatcaaaa ttcatctttt
    12181 catatactct ggagaaaaaa aaaaaagtaa ttgtagtaag aatgacaagt atcagttgag
    12241 aaaaaaagta acaaagagaa agctcggagg aggctcccca aaggttttat caccaatggt
    12301 ggtaccacta acccaagaac agaaaaagca ttgatatttt gaaaaaatac aatactcatc
    12361 ttttaacccg aaccttttct tgtcctttaa aacttttatc tcatatctgt ccaaagtcat
    12421 attgtcttac tactttctct ccttcatcgt ccttccggct ttccccgtca agctctaaat
    12481 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    12541 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    12601 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    12661 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    12721 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
    12781 attt
//
