LOCUS       sequence-23353-e         520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang
            X, Civin CI, Cheng L
  TITLE     Lentiviral vectors with two independent internal promoters transfer 
            high-level expression of multiple transgenes to human hematopoietic 
            stem-progenitor cells.
  JOURNAL   Mol Ther. 2003 Jun . 7(6):827-38.
  PUBMED    12788657
REFERENCE   2  (bases 1 to 520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2003 Jun . 7(6):827-38."
COMMENT     Sequence Label: EF.CMV.RFP
FEATURES             Location/Qualifiers
     source          1..520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(192..212)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cggtcgccac catggcctcc tccgagaacg tcatcaccga gttcatgcgc ttcaaggtgc
       61 gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      121 cctacgaggg ccacnacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg
      181 cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg
      241 ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      301 tgaacttcga ggacggcggc gtggcgaccg tgacccagga ctcctccctg caggacggct
      361 gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc
      421 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
      481 tgaagggcga gacccacaag gccctgaagc tgaaggacgg
//
