LOCUS       sequence-233619-       10545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC
  TITLE     Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives 
            chromatin association.
  JOURNAL   Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 
            10.1093/nar/gky1290.
  PUBMED    30597065
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290."
COMMENT     Sequence Label: Fuw CBX8 dCD
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        452..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             668..848
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    895..1020
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1513..1746
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1931..1975
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2273..2390
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2447..2465,2466..2488,2489..2507,2508..2530,2531..2549,
                     2550..2572,2573..2591,2592..2614,2615..2633,2634..2656,
                     2657..2675,2676..2698,2699..2717)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2750..2788
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2915..2956
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        3927..4426
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3962..3985)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4359..4378
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4447..5046
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4447..4466)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4943..4963
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5070..5658
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5123..5143)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5541..5552)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5661..5677)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5662..5678)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6183..6363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6389..6406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6395..6619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6665..7093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6752..6771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6962..6983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7102..7122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7107..7436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7287..7422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7349..7368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7484..7531
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7550..7924
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8054..8175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8091..8110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8145..8164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8224..8240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8224..8240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8237..8259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8248..8264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8272..8278,8279..8296,8297..8302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8317..8338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8455..8472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8706..8725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9385..10176,10177..10245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10008..10027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10246..10350)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10425..10444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
       61 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      121 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      181 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      241 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      301 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      361 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      421 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      481 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      541 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      601 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      661 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1381 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1441 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1501 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1561 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1621 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1681 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1741 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1801 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1861 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1921 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1981 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2041 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2101 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2161 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2221 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2281 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2341 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2401 acagggacag cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat
     2461 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2521 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2581 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2641 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2701 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
     2761 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
     2881 cgaattgcta gccgatggat ccgtggccac catgggtaag cctatcccta accctctcct
     2941 cggtctcgat tctacgggcg gaaaaacctt cctcctcaaa gcgcaggcca aggcaaaggc
     3001 caaaacttac gagtttcgaa gtgactcagc caggggcatc cggatcccct accctggccg
     3061 ctcgccccag gacctggcct ccacttcccg ggcccgggag ggccttcgaa acatgggttt
     3121 gtccccgcca gcgagcagca ccagcaccag cagcacctgc cgcgcagagg cccctcggga
     3181 ccgggaccga gaccgggata gggaccggga gcgggatcga gaaagggaga gggagcgaga
     3241 gagggagcgg gaacgtgaga gggaacgaga gcggggtacc agcagagtgg atgacaagcc
     3301 cagctcaccg ggggacagct cgaagaagcg aggccccaag ccccggaagg agctcccgga
     3361 cccctcacag aggcccttag gcgaacccag cgccggcctc ggagagtacc tcaagggcag
     3421 gaagctggac gacacccctt ccggggcagg aaagtttcca gccggccaca gtgtgatcca
     3481 gctggcccga agacaggact cggacctggt gcagtgtggt gtgaccagcc ctagctcagc
     3541 tgaggccacg ggcaaactgg ctgtggacac cttcccggcc agggtgataa agcacagggc
     3601 tgccttcctg gaggccaaag gccagggtgc cctagatccc aatggcaccc gggtccgaca
     3661 tggctcaggc ccccccagct ctgggggggg cctgtaccgg gacatggggg cccagggggg
     3721 aaggccctcc ctcatcgcca ggatccctgt ggccagaatc ctgggggacc cggaggaaga
     3781 gtcctggagc ccctccctga ctaacctgga gaaggtggtg gtcacggacg tgacctcaaa
     3841 ctttttgacc gtcaccatta aggaaagtaa cacggaccaa ggctttttta aagagaaaag
     3901 ataataagaa ttccataccg gttaccgggt aggggaggcg cttttcccaa ggcagtctgg
     3961 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     4021 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     4081 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     4141 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     4201 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     4261 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     4321 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     4381 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag
     4441 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4501 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4561 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     4621 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     4681 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     4741 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     4801 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     4861 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     4921 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     4981 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5041 gcctgaaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     5101 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5161 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5221 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5281 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5341 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5401 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5461 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5521 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5581 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5641 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     5701 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     5761 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     5821 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5881 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5941 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     6001 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     6061 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     6121 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     6181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6361 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6421 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6481 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6541 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6601 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6661 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7081 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7201 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7261 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7321 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7381 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7441 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     7501 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     7561 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     7621 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     7681 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     7741 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     7801 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     7861 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7921 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7981 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8041 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8161 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     8221 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8281 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8341 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8401 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8821 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10381 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    10441 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    10501 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaat
//
