LOCUS       sequence-233621-       10770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC
  TITLE     Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives 
            chromatin association.
  JOURNAL   Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 
            10.1093/nar/gky1290.
  PUBMED    30597065
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290."
COMMENT     Sequence Label: Fuw CBX8 3xRA
FEATURES             Location/Qualifiers
     source          1..10770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        104..483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             701..881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    928..1053
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1546..1779
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1964..2008
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2306..2423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2480..2498,2499..2521,2522..2540,2541..2563,2564..2582,
                     2583..2605,2606..2624,2625..2647,2648..2666,2667..2689,
                     2690..2708,2709..2731,2732..2750)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2783..2821
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2818..2842
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2939..2948
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2948..2989
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4185..4684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4220..4243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4617..4636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4705..5304
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4705..4724)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5201..5221
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5328..5916
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5381..5401)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5799..5810)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5919..5935)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5920..5936)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6441..6621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6647..6664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6653..6877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6923..7351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7010..7029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7220..7241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7360..7380)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7365..7694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7545..7680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7607..7626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7742..7789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7808..8182
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8312..8433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8349..8368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8403..8422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8482..8498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8482..8498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8495..8517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8506..8522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8530..8536,8537..8554,8555..8560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8575..8596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8713..8730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8884..9472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8964..8983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9643..10434,10435..10503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10266..10285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10504..10608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10683..10702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
       61 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      661 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
      721 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      781 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      841 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      901 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      961 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     1021 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1081 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1141 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1201 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1261 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1321 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1381 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1441 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1501 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1561 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1621 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1681 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1741 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1801 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1861 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1921 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1981 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2041 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2101 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2161 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2221 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     2281 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2341 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2401 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     2461 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2521 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2581 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2761 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     2821 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2881 aagacaccgg gaccgatcca gcctccgcgg ccccgaattg ctagccgatg gatccgtggc
     2941 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgg gcggagagct
     3001 ttcagcggtg ggggagcggg tgttcgcggc cgaagccctc ctgaaggccg ccatagccaa
     3061 aggacgcatg gaatacctcg tgaaatggaa gggatggtcg cagaagtaca gcacatggga
     3121 accggaggaa aacatcctgg atgctcgctt gctcgcagcc tttgaggaaa gggaaagaga
     3181 gatggagctc tatggcccca aaaagcgtgg acccaagccc aaaaccttcc tcctcaaagc
     3241 gcaggccaag gcaaaggcca aaacttacga gtttcgaagt gactcagcca ggggcatccg
     3301 gatcccctac cctggccgct cgccccagga cctggcctcc acttcccggg cccgggaggg
     3361 ccttcgaaac atgggtttgt ccccgccagc gagcagcacc agcaccagca gcacctgccg
     3421 cgcagaggcc cctcgggacc gggaccgaga ccgggatagg gaccgggagc gggatcgaga
     3481 aagggagagg gagcgagaga gggagcggga acgtgagagg gaacgagagc ggggtaccag
     3541 cagagtggat gacaagccca gctcaccggg ggacagctcg aagaagcgag gccccaagcc
     3601 ccggaaggag ctcccggacc cctcacagag gcccttaggc gaacccagcg ccggcctcgg
     3661 agagtacctc aagggcagga agctggacga caccccttcc ggggcaggaa agtttccagc
     3721 cggccacagt gtgatccagc tggcccgaag acaggactcg gacctggtgc agtgtggtgt
     3781 gaccagccct agctcagctg aggccacggg caaactggct gtggacacct tcccggccag
     3841 ggtgataaag cacagggctg ccttcctgga ggccaaaggc cagggtgccc tagatcccaa
     3901 tggcacccgg gtccgacatg gctcaggccc ccccagctct ggggggggcc tgtaccggga
     3961 catgggggcc caggggggaa ggccctccct catcgccagg atccctgtgg ccagaatcct
     4021 gggggacccg gaggaagagt cctggagccc ctccctgact aacctggaga aggtggtggt
     4081 cacggacgtg acctcaaact ttttgaccgt caccattaag gaaagtaaca cggaccaagg
     4141 cttttttaaa gagaaaagat aataagaatt ccataccggt taccgggtag gggaggcgct
     4201 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     4261 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     4321 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     4381 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     4441 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     4501 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     4561 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     4621 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     4681 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4741 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4801 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4861 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4921 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4981 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5041 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5101 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5161 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5221 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5281 atgacccgca agcccggtgc ctgaaattcg atatcaagct tatcgataat caacctctgg
     5341 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5401 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5461 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5521 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5581 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5641 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5701 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5761 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5821 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5881 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     5941 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6001 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6061 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6121 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6181 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     6241 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6301 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6361 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6421 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6601 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6901 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7321 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7621 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7681 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7741 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7801 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7861 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7921 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7981 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     8041 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     8101 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     8161 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     8221 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8281 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8401 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8461 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9301 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10621 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    10681 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    10741 gaggtcgctg agtagtgcgc gagcaaaatt
//
