LOCUS       sequence-233622-       10770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC
  TITLE     Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives 
            chromatin association.
  JOURNAL   Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 
            10.1093/nar/gky1290.
  PUBMED    30597065
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290."
COMMENT     Sequence Label: Fuw CBX8 R19A
FEATURES             Location/Qualifiers
     source          1..10770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(13..31,32..54,55..73,74..96,97..115,116..138,139..157,
                     158..180,181..199,200..222,223..241,242..264,265..283)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        316..354
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     351..375
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      472..481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             481..522
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        1718..2217
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1753..1776)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2150..2169
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2238..2837
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2238..2257)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2734..2754
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2861..3449
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2914..2934)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3332..3343)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3452..3468)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3453..3469)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3974..4154
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4180..4197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4186..4410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4456..4884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4543..4562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4753..4774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4893..4913)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4898..5227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5078..5213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5140..5159
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5275..5322
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5341..5715
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5845..5966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5882..5901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5936..5955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6015..6031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6015..6031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6028..6050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6039..6055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6063..6069,6070..6087,6088..6093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6108..6129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6246..6263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6417..7005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6497..6516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7176..7967,7968..8036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7799..7818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8037..8141)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8216..8235)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8407..8786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8788..8986
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9004..9184
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9231..9356
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9849..10082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10267..10311
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10609..10726
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
       61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
      301 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      421 cgggaccgat ccagcctccg cggccccgaa ttgctagccg atggatccgt ggccaccatg
      481 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgggcggaga gctttcagcg
      541 gtgggggagc gggtgttcgc ggccgaagcc ctcctgaagg cccgcatacg gaaaggacgc
      601 atggaatacc tcgtgaaatg gaagggatgg tcgcagaagt acagcacatg ggaaccggag
      661 gaaaacatcc tggatgctcg cttgctcgca gcctttgagg aaagggaaag agagatggag
      721 ctctatggcc ccaaaaagcg tggacccaag cccaaaacct tcctcctcaa agcgcaggcc
      781 aaggcaaagg ccaaaactta cgagtttcga agtgactcag ccaggggcat ccggatcccc
      841 taccctggcc gctcgcccca ggacctggcc tccacttccc gggcccggga gggccttcga
      901 aacatgggtt tgtccccgcc agcgagcagc accagcacca gcagcacctg ccgcgcagag
      961 gcccctcggg accgggaccg agaccgggat agggaccggg agcgggatcg agaaagggag
     1021 agggagcgag agagggagcg ggaacgtgag agggaacgag agcggggtac cagcagagtg
     1081 gatgacaagc ccagctcacc gggggacagc tcgaagaagc gaggccccaa gccccggaag
     1141 gagctcccgg acccctcaca gaggccctta ggcgaaccca gcgccggcct cggagagtac
     1201 ctcaagggca ggaagctgga cgacacccct tccggggcag gaaagtttcc agccggccac
     1261 agtgtgatcc agctggcccg aagacaggac tcggacctgg tgcagtgtgg tgtgaccagc
     1321 cctagctcag ctgaggccac gggcaaactg gctgtggaca ccttcccggc cagggtgata
     1381 aagcacaggg ctgccttcct ggaggccaaa ggccagggtg ccctagatcc caatggcacc
     1441 cgggtccgac atggctcagg cccccccagc tctggggggg gcctgtaccg ggacatgggg
     1501 gcccaggggg gaaggccctc cctcatcgcc aggatccctg tggccagaat cctgggggac
     1561 ccggaggaag agtcctggag cccctccctg actaacctgg agaaggtggt ggtcacggac
     1621 gtgacctcaa actttttgac cgtcaccatt aaggaaagta acacggacca aggctttttt
     1681 aaagagaaaa gataataaga attccatacc ggttaccggg taggggaggc gcttttccca
     1741 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     1801 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     1861 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     1921 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     1981 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     2041 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     2101 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     2161 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     2221 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     2281 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     2341 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     2401 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     2461 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     2521 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     2581 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     2641 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     2701 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     2761 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     2821 gcaagcccgg tgcctgaaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2881 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2941 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3001 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3061 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3121 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3181 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3241 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3301 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3361 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3421 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     3481 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     3541 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     3601 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3661 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     3721 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     3781 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     3841 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     3901 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     3961 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4021 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4081 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4141 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4201 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4261 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4321 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4381 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4441 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4501 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4561 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4621 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4681 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4741 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4861 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4921 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4981 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5041 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5101 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5161 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5221 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     5281 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     5341 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     5401 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     5461 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     5521 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     5581 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     5641 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     5701 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5761 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5821 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5941 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6001 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8161 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     8221 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     8281 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     8341 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     8401 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     8461 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8521 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     8581 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     8641 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     8701 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     8761 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     8821 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     8881 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     8941 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     9001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9181 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     9241 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     9301 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9361 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9421 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9481 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9541 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9601 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9661 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     9721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     9781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     9841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     9901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     9961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    10381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    10501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
    10561 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
    10621 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    10681 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    10741 ggacagcaga gatccagttt ggttagatct
//
