LOCUS       sequence-233630-        6503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            flagellin promoter driven mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_PflaB-mCitrineBb
FEATURES             Location/Qualifiers
     source          1..6503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    467..488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(503..508,509..526,527..533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    541..557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     546..568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     565..581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     565..581
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1686..1703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1686..1702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1695..1717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2269..2802
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      2905..3493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3394..3413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatttt gctacttgat attggatcgt aaaaatatta gggctttaca aagttctttt
       61 aaaagagaat ttagtaaagc cctaagtctt ttaataaaaa attttttgta aaaaagttgg
      121 caaaaatagt ttttgctata tatttatata taagaaaaaa ttcaatactc aaggagataa
      181 aaaatggaaa acttaaacaa taatccacaa aaaattaatc aaataaataa gcaaactaat
      241 tttattggtt ttgaaaattt tatagaactt gataatcaag ttaatcaaac taaagaaaaa
      301 agtaaattaa gtaagatagg taagaaatta cccggaataa gcagtcaagt ctagtgcggc
      361 cgcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      421 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      481 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      541 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattcgagct
      601 ctgtctgtcg cctcttgtgg cttccggagg agttatttat aataaaataa agaattacga
      661 cttgaaaatt tttaatcaaa aaaataaagt tgaagattat aaaaagaagt acgaagatag
      721 agagagaaaa gtggaaggtg aacttaatac cttggatttt accgttaagc gcatgaaaga
      781 tcaagaaaat acattaaagg cttttgattt taatcaaaga aataaataat aataattatt
      841 tttaatgcta ttgctatttg cgtttctttt tttttaattt ttgtgctatt ctttttaaca
      901 ggcaaaagga tttgccaaag tcagaaattt aaattttatc atggaggaat gaggatccat
      961 ggttagtaaa ggtgaagaat tatttactgg tgttgttcct attttagttg aattagatgg
     1021 tgatgttaat ggtcataaat ttagtgttag tggtgaaggt gaaggtgatg ctacttatgg
     1081 taaattaact ttaaaattta tttgtactac tggtaaatta cctgttcctt ggcctacttt
     1141 agttactact tttggatatg gtttaatgtg ttttgctaga tatcctgatc atatgaaaca
     1201 acatgatttt tttaaaagtg ctatgcctga aggttatgtt caagaaagga ctattttctt
     1261 caaagatgat ggtaattata aaactagagc tgaagttaaa tttgaaggtg atactttagt
     1321 taatagaatt gaattaaaag gtattgattt taaagaagat ggtaatattt taggtcataa
     1381 attagaatat aattataata gtcataatgt ttatattatg gctgataaac aaaaaaatgg
     1441 tattaaagtt aattttaaaa ttagacataa tattgaagat ggtagtgttc aattagctga
     1501 tcattatcaa caaaatactc ctattggtga tggtcctgtt ttattacctg ataatcatta
     1561 tttaagttat caaagtaaat taagtaaaga tcctaatgaa aaaagagatc atatggtttt
     1621 attagaattt gttactgctg ctggtattac tttaggtatg gatgaattat ataaataagc
     1681 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcggccg ccctaggtta
     1861 atacccgagc ttcaaggaag atttcctatt aaggttgaac ttaagagctt aagcatagac
     1921 gatttgaaaa aaattttaaa acaaacaaaa aattctttaa taaaacaata tgttgcgatg
     1981 tttaaggttt atgatttaga tttaaagttt agtgaggaag ctatagatag aattgcagag
     2041 cttactttta atatgaatct tgagagtgaa aatcttggtg ccagaagact tcacggtgtt
     2101 atggaaatag tgcttgcaga tctttttttt gaagtgcctg gcagtaagtt gaaaaaattt
     2161 gaaataaact tggactatgt taataaaaaa atacaaatta acgaacaaaa agatttgaat
     2221 tattatataa tttagttaaa agcaatttta aatgagggag gtttccatat gttacgcagc
     2281 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg
     2341 ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt
     2401 gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc
     2461 gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa
     2521 gaagcggttg ttggcgctct cgcggcttac gttctgccca agtttgagca gccgcgtagt
     2581 gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc
     2641 gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg
     2701 caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa
     2761 gaagtgatgc actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag
     2821 atcggcttac gcgtccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3541 ttttgctcac atgttctttc ctgcactaga tccccggcaa gttcatcctt agggttttca
     3601 tatctcttaa aaagtcaatt tctgcttgat taatttcttg tggattattg tttttgcggt
     3661 ttttcatttt ttatctcctt gagtattgaa ttttttctta tatataaaat atagcaaaaa
     3721 ctatttttgc caactttttt acaaaaaatt ttttattaaa acacttaggg ctttgctaaa
     3781 ttctctttta aaagaactta gtaaagccct atggatttaa gaactgatta tacttacgta
     3841 gtaaaaatac tacagatatt aatagtatag accatatcaa aatcataatc aatttatatt
     3901 tatctaaaat tgtggtccat acaaaataca aaaaaattag gtttttctaa attctatttt
     3961 aaaagaactt agtaacacct aatctctaaa ttacttacaa aattctaaaa ttagattttt
     4021 agttcttcat atttctttaa aagtttttta agaaaatctt tttgattttc aaaaatttca
     4081 tgcatcataa aactagtaaa tttggatttt tttttgtaaa aatcataact ttcttgggtt
     4141 ttaagttgaa atcttaatgg ttttattgga ttttgcctag atttttttaa cgccggactt
     4201 tctttatctt ttataaaatt ataagaattt ttaataccat tttctattaa ataatcttct
     4261 tttataagcc cctcttgcag tgcagttgct aatcttaaat acttatacgc ttgagttttt
     4321 gctacatcaa agtcttttat aaaatcaaga aaagttttat aaccatcaaa tttataatat
     4381 tgattatctt ttatttcttt taaaatttta atattttcca ccctgtggaa tatttctttt
     4441 ttgaaattgt catttaactt ttcctttagt gtcttgtaat aagaaatttt actttcatta
     4501 atcattaaat tttgttcaac ataagtatct atcttttttg ttaaaacttt aacaacacgt
     4561 tttttttcca taaaaatttt cctttatttt tgcaacattt tccttaattg atataattgt
     4621 tactatttta gattttttaa tatccactta agaattaatc cctcactaga atatttttta
     4681 ttatagaatt catatacatg tttttctaaa tctataaatt tatcaacaat tgctttatta
     4741 tagggtgtat ctatcttttc ttttcttaat aatcgtctca tattctttaa ataacaacaa
     4801 acactgccgg ttttaaacct aaattctatg taatatattt ttgaaaaagt atatacattt
     4861 tcaaccccgt ttatttcata ctttacaaaa acattattta ttggttttct ataaccatag
     4921 taaatgccta aaaatttgtc cccgtctttc ataggatata gataaagttt ttgaatctct
     4981 gatttatcta ataatttttc aaaaaaaatt atacaccgct tttgtttgtt actaactact
     5041 ctatgcaatg cctttaatat ttttgtgtga tatatatttt tatcaccttt tttttctttc
     5101 agcaggaaga tactttcttt tggaaattta attttttgtt catttattct tgcagcctta
     5161 agtccgattt tcatatttaa tccttatata gcctttctat ttaattcttt taaactcaat
     5221 aaactttttt ggtttttcat tagttctaat aattcgcaat aatatgtgca aaacacttta
     5281 ttgtattcta ttttcttttg gttattcaaa tattctttta taattggctt tagaagttca
     5341 atatttactt ctttttttag ttgttcaata aggatattga aaatgtttgt ttttaaatct
     5401 tgataatttt gttgagaatt ttcttttttt ctttcaattg acttctctaa tttacgcttt
     5461 atattgttta aatcgctata tttatgactt tcaataataa aatgaggctt aaatttgtaa
     5521 ttttcgtata ttttttggaa acttgtttct aattgtttag aattatattc atttttttca
     5581 aattcttttt gagtgttgca tagaatttcc tttaattttt cttgtttttc tttcatacaa
     5641 gatttagtaa aatgtatatt tttatttttt agtaatttaa tttctattat ttttatgatt
     5701 ttaagtattt tgattatatt atctttatca atatttaaat tgaataaaat tgtaagaaaa
     5761 attgaaagaa tttttttaca taaaaagtta catttattga agtaatttct tacttgatac
     5821 ttttctattt cattaatctt tctttcttct tttatattat ttttattatt taaacactcc
     5881 tttaaatcta cactcccatt tttagtaaat ctatctttaa ggccccttga aactctattt
     5941 tgaaatctta aatctttttt ttcttgaaag tatttataga ttttttggta acattctttt
     6001 tttgaaaaat taagttgata gtatatttcg gtccccaaat ttacccctaa gtgtttgtga
     6061 taattagttg tgactttaaa ttctttttct aatttgtaaa ggtaattttg cagtgttttt
     6121 agtttaacgg gtgtttgact atttctttta aggttttcat taaagtaata gagtatgttt
     6181 ttttgtgtgt atttcttata tgttttattt atgtacgcta gtgttgagat aaggactact
     6241 aatttatact gtaatttatt gtagcaagtt gtggttttac tggttttgtt tatattagat
     6301 aagttttcca tttatagctc cttgaatacc ggatttttta tctataaagt atatatatca
     6361 caattttgat ttaaaagtaa attctttttt taaaaataca aaaatttttt ataaattata
     6421 tagaccatga tattgtatta atactaattg attttaattt aaggttgaac taaaataaac
     6481 atagttttgt agaaaataat ttt
//
