LOCUS       sequence-233633-        6679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            resT promoter driven mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2_PresT-mCherryBb
FEATURES             Location/Qualifiers
     source          1..6679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1018..1035)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1018..1034)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1027..1049)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1601..2410
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MGHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1668..1687)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2579..3167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3068..3087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6644..6665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgaca tgattacgaa ttcgagctcc gaagtttatt attttatgat
      121 ttaaattaat aaaaagattt atcggtgtga atagaatatt tctttggttt attatgcatc
      181 ctagtacata ttatataatt taatttaata tataagcata tttttttaaa atcagaaatt
      241 ttttacttag ctaaagattt taaacttggt ataattgaat taatggatcc aggaggttca
      301 tggttagtaa aggtgaagaa gataatatgg ctattattaa agaatttatg agatttaaag
      361 ttcacatgga aggtagtgtt aatggtcatg aatttgaaat tgaaggtgaa ggtgaaggta
      421 gaccttatga aggtactcaa actgctaaat taaaagttac taaaggtggt cctttacctt
      481 ttgcttggga tattttaagt cctcaattta tgtatggtag taaagcatac gttaaacatc
      541 ctgctgatat tcctgattat ttaaaattaa gttttcctga aggttttaaa tgggaaagag
      601 ttatgaattt tgaagatggt ggtgttgtta ctgttactca agatagtagt ttacaagatg
      661 gtgaatttat ttataaagtt aaattaagag gtactaattt tcctagtgat ggtcctgtta
      721 tgcaaaaaaa aactatgggt tgggaagcta gtagtgaaag aatgtatcct gaagatggtg
      781 ctttaaaagg tgaaattaaa caaagattaa aattaaaaga tggtggtcat tatgatgctg
      841 aagttaaaac tacttataaa gctaaaaaac ctgttcaatt acctggtgct tataatgtta
      901 atattaaatt agatattact tcgcataatg aagattatac tattgttgaa caatatgaaa
      961 gagctgaagg tagacatagt acaggtggta tggatgaatt atataaataa gcttggcact
     1021 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     1081 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     1141 ttcccaacag ttgcgcagcc tgaatggcga atggcgcggc cgccctaggt taatacccga
     1201 gcttcaagga agatttccta ttaaggttga acttaagagc ttaagcatag acgatttgaa
     1261 aaaaatttta aaacaaacaa aaaattcttt aataaaacaa tatgttgcga tgtttaaggt
     1321 ttatgattta gatttaaagt ttagtgagga agctatagat agaattgcag agcttacttt
     1381 taatatgaat cttgagagtg aaaatcttgg tgccagaaga cttcacggtg ttatggaaat
     1441 agtgcttgca gatctttttt ttgaagtgcc tggcagtaag ttgaaaaaat ttgaaataaa
     1501 cttggactat gttaataaaa aaatacaaat taacgaacaa aaagatttga attattatat
     1561 aatttagtta aaagcaattt taaatgaggg aggtttccat atgggccata ttcaacggga
     1621 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1681 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1741 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1801 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1861 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1921 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1981 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2041 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2101 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2161 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2221 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2281 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2341 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2401 gtttttctaa tcagactcga gtggccgagg agcaggactg acacgtgcta aaacttcatt
     2461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2641 cggtggtttg tttgccggat caagagctac caactctttt ttcgaaggta actggcttca
     2701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcac
     3241 tagatccccg gcaagttcat ccttagggtt ttcatatctc ttaaaaagtc aatttctgct
     3301 tgattaattt cttgtggatt attgtttttg cggtttttca ttttttatct ccttgagtat
     3361 tgaatttttt cttatatata aatatatagc aaaaactatt tttgccaact tttttacaaa
     3421 aaatttttta ttaaaacact tagggctttg ctaaattctc ttttaaaaga acttagtaaa
     3481 gccctatgga tttaagaact gattatactt acgtagtaaa atactacaga tattaatagt
     3541 atagaccata tcaaaatcat aatcaattta tatttatcta aaattgtagt ccatacaaaa
     3601 tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga acttagtaac acctaatctc
     3661 taaattactt acaaaattct aaaattagat ttttagttct tcatatttct ttaaaagttt
     3721 tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc ataaaactag taaatttgga
     3781 tttttttttg taaaaatcat aactttcttg ggttttaagt tgaaatctta atggttttat
     3841 tggattttgc ctagattttt ttaacgccgg actttcttta tcttttataa aattataaga
     3901 atttttaata ccattttcta ttaaataatc ttcttttata agcccctctt gcagtgcagt
     3961 tgctaatctt aaatacttat acgcttgagt ttttgctaca tcaaagtctt ttataaaatc
     4021 aagaaaagtt ttataaccat caaatttata atattgatta tcttttattt cttttaaaat
     4081 tttaatattt tccaccctgt ggaatatttc ttttttgaaa ttgtcattta acttttcctt
     4141 tagtgtcttg taataagaaa ttttactttc attaatcatt aaattttgtt caacataagt
     4201 atctatcttt tttgttaaaa ctttaacaac acgttttttt tccataaaaa ttttccttta
     4261 tttttgcaac attttcctta attgatataa ttgttactat tttagatttt ttaatatcca
     4321 cttaagaatt aatccctcac tagaatattt tttattatag aattcatata catgtttttc
     4381 taaatctata aatttatcaa caattgcttt attatagggt gtatctatct tttcttttct
     4441 taataatcgt ctcgtattct ttaaataaca acaaacactg ccggttttaa acctaaattc
     4501 tatgtaatat atttttgaaa aagtatatac attttcaacc ccgtttattt catactttac
     4561 aaaaacatta tttattggtt ttctataacc atagtaaatg cctaaaaatt tgtccccgtc
     4621 tttcatagga tatagataaa gtttttgaat ctctgattta tctaataatt tttcaaaaaa
     4681 aattatacac cgcttttgtt tgttactaac tactctatgc aatgccttta atatttttgt
     4741 gtgatatata tttttatcac cttttttttc tttcagcagg aagatacttt cttttggaaa
     4801 tttaattttt tgttcattta ttcttgcagc cttaagtccg attttcatat ttaatcctta
     4861 tatagccttt ctatttaatt cttttaaact caataaactt ttttggtttt tcattagttc
     4921 taataattcg caataatatg tgcaaaacac tttattgtat tctattttct tttggttatt
     4981 caaatattct tttataattg gctttagaag ttcaatattt acttcttttt ttagttgttc
     5041 aataaggata ttgaaaatgt ttgtttttaa atcttgataa ttttgttgag aattttcttt
     5101 ttttctttca attgacttct ctaatttacg ctttatattg tttaaatcgc tatatttatg
     5161 actttcaata ataaaatgag gcttaaattt gtaattttcg tatatttttt ggaaacttgt
     5221 ttctaattgt ttagaattat attcattttt ttcaaattct ttttgagtgt tgcatagaat
     5281 ttcctttaat ttttcttgtt tttctttcat acaagattta gtaaaatgta tatttttatt
     5341 ttttagtaat ttaatttcta ttatttttat gattttaagt attttgatta tattatcttt
     5401 atcaatattt aaattgaata aaattgtaag aaaaattgaa agaatttttt tacataaaaa
     5461 gttacattta ttgaagtaat ttcttacttg atacttttct atttcattaa tctttctttc
     5521 ttcttttata ttatttttat tatttaaaca ctcctttaaa tctacactcc catttttagt
     5581 aaatctatct ttaaggcccc ttgaaactct attttgaaat cttaaatctt ttttttcttg
     5641 aaagtattta tagatttttt ggtaacattc tttttttgaa aaattaagtt gatagtatat
     5701 ttcggtcccc aaatttaccc ctaagtgttt gtgataatta gttgtgactt taaattcttt
     5761 ttctaatttg taaaggtaat tttgcagtgt ttttagttta acgggtgttt gactatttct
     5821 tttaaggttt tcattaaagt aatagagtat gtttttttgt gtgtatttct tatatgtttt
     5881 atttatgtac gctagtgttg agataaggac tactaattta tactgtaatt tattgtagca
     5941 agttgtggtt ttactggttt tgtttatatt agataagttt tccatttata gctccttgaa
     6001 taccggattt tttatctata aagtatatat atcacaattt tgatttaaaa gtaaattctt
     6061 tttttaaaaa tacaaaaatt ttttataaat tatatagacc atgatattgt attaatacta
     6121 attgatttta atttaaggtt gaactaaaat aaacatagtt ttgtagaaaa taatttttca
     6181 ttattttgct acttgatatt ggatcgtaaa aatattaggg ctttacaaag ttcttttaaa
     6241 agagaattta gtaaagccct aagtctttta ataaaaaatt ttttgtaaaa aagttggcaa
     6301 aaatagtttt tgctatatat ttatatataa gaaaaaattc aatactcaag gagataaaaa
     6361 atggaaaact taaacaataa tccacaaaaa attaatcaaa taaataagca aactaatttt
     6421 attggttttg aaaattttat agaacttgat aatcaagtta atcaaactaa agaaaaaagt
     6481 aaattaagta agataggtaa gaaattaccc ggaataagca gtcaagtcta gtgcggccgc
     6541 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6601 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6661 ctcattaggc accccaggc
//
