LOCUS       sequence-233635-        8924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BvCas12b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, 
            Makarova KS, Koonin EV, Zhang F
  TITLE     Engineering of CRISPR-Cas12b for human genome editing.
  JOURNAL   Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4.
  PUBMED    30670702
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-22"; volume: "10(1):212. doi"; pages: " 
            10.1038/s41467-018-08224-4"
COMMENT     Sequence Label: pZ143-pcDNA3-BvCas12b
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(65..653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(145..164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(824..1615,1616..1684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1447..1466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1685..1789)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1864..1883)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2683..2702
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2683..2701
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2737..2746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2749..2769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6127..6174
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6181..6261
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(6335..6352)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6341..6565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6611..7039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6698..6717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6908..6929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7048..7068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7053..7382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7233..7368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7295..7314
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7449..8243
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7503..7522)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8113..8132
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8417..8538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8454..8473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8508..8527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8587..8603)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8587..8603)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8600..8622)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8611..8627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8635..8641,8642..8659,8660..8665))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8680..8701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8818..8835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
       61 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      181 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      241 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      301 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      361 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      421 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      481 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      541 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      601 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      661 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      721 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      781 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      841 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      901 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      961 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1021 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1081 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1141 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1201 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1261 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1321 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1381 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1441 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1501 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1561 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1621 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1681 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1741 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1801 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     1861 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     1921 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     1981 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     2041 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     2641 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     2701 ggagacccaa gctggctagc gtttaaactt aagcttgcca ccatggcccc aaagaagaag
     2761 cggaaggtcg gtatccacgg agtcccagca gccgccatcc ggtccatcaa gctgaagatg
     2821 aagaccaaca gcggcaccga cagcatctac ctgagaaaag ccctgtggcg gacccaccag
     2881 ctgatcaatg agggaatcgc ctactacatg aacctgctga ccctgtaccg gcaagaggcc
     2941 atcggcgaca agaccaaaga agcctatcag gccgagctga ttaacatcat ccggaaccag
     3001 cagcggaaca acggcagctc tgaggaacac ggctccgacc aagaaattct ggccctgctg
     3061 agacagctgt acgagctgat catccccagc agcatcggcg aatctggcga cgctaatcag
     3121 ctgggcaaca agtttctgta ccctctggtg gaccccaaca gccagtctgg caagggcaca
     3181 tctaacgccg gcagaaagcc cagatggaag cggctgaaag aggaaggcaa ccccgactgg
     3241 gaactcgaga agaagaagga cgaggaacgc aaggccaagg atcccaccgt gaagatcttt
     3301 gacaacctga acaaatacgg cctgctgcct ctgttcccac tgttcaccaa catccagaaa
     3361 gacatcgagt ggctgcccct gggcaagaga cagtctgtgc ggaagtggga caaagacatg
     3421 ttcatccagg ccatcgagag actgctgagc tgggagagct ggaacagaag agtggccgac
     3481 gagtacaaac agctgaaaga aaagaccgag agctactaca aagagcacct gacaggcggc
     3541 gaggaatgga tcgagaagat ccggaagttc gagaaagaac ggaacatgga actggaaaag
     3601 aacgccttcg ctcccaacga cggctacttc atcaccagca gacagatcag aggctgggac
     3661 agagtgtacg agaagtggtc caagctgccc gagtctgcta gccctgagga actgtggaaa
     3721 gtggtggccg agcagcagaa caagatgtcc gaaggcttcg gcgaccccaa ggtgttcagc
     3781 ttcctggcca acagagagaa ccgggacatt tggagaggcc acagcgagcg gatctaccac
     3841 attgccgcct acaacggcct gcagaagaag ctgagccgga ccaaagagca ggccaccttc
     3901 acactgcctg acgccattga acaccctctg tggatcagat acgagagccc tggcggcacc
     3961 aacctgaatc tgttcaagct ggaagagaaa cagaaaaaga actactacgt gaccctgagc
     4021 aagatcatct ggcccagcga ggaaaagtgg attgagaaag agaacatcga gatccctctg
     4081 gctcccagca tccagttcaa ccggcagatt aagctgaagc agcacgtgaa gggcaagcaa
     4141 gagatcagct tcagcgacta cagcagccgg atcagcctgg atggtgttct cggcggcagc
     4201 agaatccagt ttaatcggaa gtacatcaag aaccacaaag agctgctcgg agagggcgac
     4261 atcggccccg tgttctttaa cctggtggtg gatgtggccc ctctgcaaga aaccagaaac
     4321 ggcagactgc agagccccat cggcaaggcc ctgaaagtga tcagcagcga cttctccaaa
     4381 gtgatcgact acaagccgaa agaactcatg gattggatga ataccggcag cgccagcaac
     4441 agctttggag tggcttctct gctggaaggc atgagagtga tgagcatcga catgggccag
     4501 agaaccagcg cctccgtgtc catcttcgag gtcgtgaaag aactgcccaa ggatcaagag
     4561 cagaagctgt tctacagcat caacgacacc gagctgttcg ccatccacaa gcggagcttt
     4621 ctgctgaacc tgcctggcga ggtggtcacc aagaacaaca agcagcagcg gcaagagcgg
     4681 cggaaaaagc ggcagtttgt gcggagccag atcagaatgc tggccaacgt gctgcggctg
     4741 gaaacaaaga aaacccctga cgagcggaag aaggccattc acaagctgat ggaaatcgtg
     4801 cagagctacg acagctggac cgccagccag aaagaagtgt gggagaaaga gctgaatctc
     4861 ctgaccaaca tggccgcctt caatgacgag atctggaaag aaagcctggt ggaactgcac
     4921 caccggatcg agccttacgt gggacagatc gtgtccaagt ggcggaaggg cctgtctgag
     4981 ggcagaaaga atctggccgg catcagcatg tggaacatcg acgaactgga agataccagg
     5041 cggctgctga tttcctggtc caagagaagc agaaccccag gcgaggccaa caggatcgaa
     5101 accgatgagc ctttcggcag cagcctgctc cagcacattc agaacgtgaa ggacgacaga
     5161 ctgaagcaga tggccaacct gatcatcatg acagccctgg gctttaagta cgacaaagag
     5221 gaaaaggacc ggtacaagcg gtggaaagag acataccccg cctgccagat catcctgttc
     5281 gagaacctga accgctacct gttcaacctc gaccggtcca gacgcgagaa cagcagactg
     5341 atgaagtggg cccatcggag catccccaga accgtgtcta tgcagggcga gatgttcggc
     5401 ctgcaagtgg gcgacgttcg gagcgagtac agctccagat tccacgccaa aacaggcgcc
     5461 cctggcatca gatgtcacgc cctgactgaa gaggatctga aggccggcag caacaccctg
     5521 aagagactga tcgaggacgg cttcatcaat gagagcgagc tggcctacct gaagaagggc
     5581 gatatcatcc ctagccaagg cggcgaactg ttcgtgacac tgtccaagcg gtacaagaag
     5641 gacagcgaca acaacgagct gaccgtgatc cacgccgaca tcaacgccgc tcagaatctg
     5701 cagaagcggt tttggcagca aaacagcgag gtgtacagag tgccctgtca gctggccaga
     5761 atgggcgaag ataagctgta catccccaag agccagaccg agacaatcaa gaagtatttc
     5821 ggcaagggct ccttcgtgaa gaacaatacc gaacaagagg tctacaagtg ggagaagtcc
     5881 gagaaaatga agatcaagac ggacaccacc ttcgacctgc aagacctgga tggcttcgag
     5941 gacatcagca agaccattga gctggcacaa gagcagcaaa agaaatacct gaccatgttc
     6001 agggacccca gcggctactt tttcaacaat gagacatggc ggcctcaaaa agaatactgg
     6061 tccatcgtga acaacatcat caagagctgc ctcaagaaga agatcctgag caacaaggtc
     6121 gagctgaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     6181 tacccatacg atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca
     6241 tatgatgtcc ccgactatgc ctaagaattc tgcagatatc cagcacagtg gcggccgctc
     6301 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6361 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6421 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6481 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6541 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     6601 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6901 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6961 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7021 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7081 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7141 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7201 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7261 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7321 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7381 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     7441 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7501 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7561 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7621 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7681 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7741 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7801 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7861 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7921 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7981 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8041 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8101 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8161 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8221 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     8281 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8341 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8401 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8521 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     8581 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8641 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8701 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaa
//
