LOCUS       sequence-233653-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector for expression of AcrIIC3 with two miR-122 binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri
            J, Mastel M, Borner K, Eils R, Grimm D, Niopek D
  TITLE     Cell-specific CRISPR-Cas9 activation by microRNA-dependent 
            expression of anti-CRISPR proteins.
  JOURNAL   Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 
            10.1093/nar/gkz271.
  PUBMED    30982889
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271."
COMMENT     Sequence Label: AAV CMV-driven AcrIIC3-2xmiR-122 target sites
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..252
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4..24
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     175..194
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        373..752
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        753..956
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     907..927
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1001..1020
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1001..1019
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1039..1048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1536..1553)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1542..1766
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
       61 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      121 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      181 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      241 ggacgaaaca ccgagtcttc gaggaagaca ggtttaagag ctatgctgga aacagcatag
      301 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      361 ttttgcggcc gcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      421 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      481 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      541 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      601 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      661 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      721 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      781 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      841 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      901 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
      961 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     1021 agacccaagc tggctagcgc caccatggct ttcaagagag ctattatatt tacgtccttt
     1081 aatggttttg agaaggtctc tcgaacagag aagcgacgcc ttgccaagat aattaacgcg
     1141 agggtctcaa ttattgatga gtatctcaga gccaaggata caaacgcgag cctggatggc
     1201 caataccgag cctttctgtt taacgacgaa agccccgcga tgacagagtt cctcgcaaaa
     1261 ctgaaagcat tcgcggagag ctgcacggga atatcaatag atgcgtggga gattgaagag
     1321 agcgagtatg ttcgactgcc cgttgaacgc cgggatttcc tggccgctgc gaatggcaag
     1381 gaaatcttca agatataact cgagcggccg ccactgtgct ggatatctgc agaattcaaa
     1441 caccattgtc acactccatt atatcaaaca ccattgtcac actccattaa ttagagacga
     1501 gctcgtctct agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca
     1561 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1621 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1681 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1741 tgctggggat gcggtgggct ctatgggtcg acgatatcag atctgaggaa cccc
//
