LOCUS       sequence-233681-       14459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Porter EG, Dykhuizen EC
  TITLE     Individual Bromodomains of Polybromo-1 Contribute to Chromatin 
            Association and Tumor Suppression in Clear Cell Renal Carcinoma.
  JOURNAL   J Biol Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875.
  PUBMED    28053089
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875."
COMMENT     Sequence Label: Fuw PBRM1 mBD2
FEATURES             Location/Qualifiers
     source          1..14459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        429..627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             645..825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    872..997
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1490..1723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1908..1952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2250..2367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2424..2442,2443..2465,2466..2484,2485..2507,2508..2526,
                     2527..2549,2550..2568,2569..2591,2592..2610,2611..2633,
                     2634..2652,2653..2675,2676..2694)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2727..2765
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2762..2786
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2888..2897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7818..8317
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7853..7876)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8250..8269
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8338..8937
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8338..8357)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8834..8854
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8961..9549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9014..9034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9432..9443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9552..9568)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9553..9569)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10074..10254
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10280..10297)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10286..10510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10556..10984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10643..10662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10853..10874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10993..11013)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10998..11327
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11178..11313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11240..11259
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11375..11422
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11441..11815
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11945..12066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11982..12001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12036..12055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12115..12131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12115..12131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12128..12150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12139..12155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12163..12169,12170..12187,12188..12193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12208..12229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12346..12363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12517..13105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12597..12616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13276..14067,14068..14136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13899..13918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14137..14241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14316..14335)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
       61 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      601 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      841 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      901 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      961 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1021 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1081 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1141 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1201 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1261 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1321 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1381 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1441 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1501 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1561 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1621 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1681 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1741 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1801 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1861 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1921 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1981 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2041 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2101 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2161 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2221 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2341 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2401 tggttagatc tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2461 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2521 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2581 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2641 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2701 aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca
     2761 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2821 atagaagaca ccgggaccga tccagcctcc gcggccccga attgctagcc gatggattcg
     2881 tgaattcgcc accatgggtt ccaagagaag aagagctacc tccccttcca gcagtgtcag
     2941 cggggacttt gatgatgggc accattctgt gtcaacacca ggcccaagca ggaaaaggag
     3001 gagactttcc aatcttccaa ctgtagatcc tattgccgtg tgccatgaac tctataatac
     3061 catccgagac tataaggatg aacagggcag acttctctgt gagctcttca ttagggcacc
     3121 aaagcgaaga aatcaaccag actattatga agtggtttct cagcccattg acttgatgaa
     3181 aatccaacag aaactaaaaa tggaagagta tgatgatgtt aatttgctga ctgctgactt
     3241 ccagcttctt tttaacaatg caaagtccta ttataagcca gattctcctg aatataaagc
     3301 cgcttgcaaa ctctgggatt tgtaccttcg aacaagaaat gagtttgttc agaaaggaga
     3361 agcagatgac gaagatgatg atgaagatgg gcaagacaat cagggcacag tgactgaagg
     3421 atcttctcca gcttacttga aggagatcct ggagcagctt cttgaagcca tagttgtagc
     3481 tacaaatcca tcaggacgtc tcattagcga actttttcag aaactgcctt ctaaagtgca
     3541 atatccagat tattatgcaa taattaagga gcctatagat ctcaagacca ttgcccagag
     3601 gatacagaat ggaagctaca aaagtattca tgcaatggcc aaagatatag atctcctcgc
     3661 aaaaaatgcc aaaacttatg ccgagcctgg ctctcaagta ttcaaggatg caaattcaat
     3721 taaaaaaata ttttatatga aaaaggctga aattgaacat catgaaatgg ctaagtcaag
     3781 tcttcgaatg aggactccat ccaacttggc tgcagccaga ctgacaggtc cttcacacag
     3841 taaaggcagc cttggtgaag agagaaatcc cactagcaag tattaccgta ataaaagagc
     3901 agtacaagga ggtcgtttat cagcaattac aatggcactt caatatggct cagaaagtga
     3961 agaagatgct gctttagctg ctgcacgcta tgaagaggga gagtcagaag cagaaagcat
     4021 cacttccttt atggatgttt caaatccttt ttatcagctt tatgacacag ttaggagttg
     4081 tcggaataac caagggcagc taatagctga acctttttac catttgcctt caaagaaaaa
     4141 ataccctgat tattaccagc aaattaaaat gcccatatca ctacaacaga tccgaacaaa
     4201 actgaagaat caagaatatg aaactttaga tcatttggag tgtgatctga atttaatgtt
     4261 tgaaaatgcc aaacgctata atgtgcccaa ttcagccatc tacaagcgag ttctaaaatt
     4321 gcagcaagtt atgcaggcaa agaagaaaga gcttgccagg agagacgata tcgaggacgg
     4381 agacagcatg atctcttcag ccacctctga tactggtagt gccaaaagaa aaagtaaaaa
     4441 gaacataaga aagcagcgaa tgaaaatctt attcaatgtt gttcttgaag ctcgagagcc
     4501 aggttcaggc agaagacttt gtgacctatt tatggttaaa ccatccaaaa aggactatcc
     4561 tgattattat aaaatcatct tggagccaat ggacttgaaa ataattgagc ataacatccg
     4621 caatgacaaa tatgctggtg aagagggaat gatagaagac atgaagctga tgttccggaa
     4681 tgccaggcac tataatgagg agggctccca ggtttataat gatgcacata tcctggagaa
     4741 gttactcaag gagaaaagga aagagctggg cccactgcct gatgatgatg acatggcttc
     4801 tcccaaactc aagctgagta ggaagagtgg catttctcct aaaaaatcaa aatacatgac
     4861 tccaatgcag cagaaactaa atgaggtcta tgaagctgta aagaactata ctgataagag
     4921 gggtcgccgc ctcagtgcca tatttctgag gcttccctct agatctgagt tgcctgacta
     4981 ctatctgact attaaaaagc ccatggacat ggaaaaaatt cgaagtcaca tgatggccaa
     5041 caagtaccaa gatattgact ctatggttga ggactttgtc atgatgttta ataatgcctg
     5101 tacgtacaat gagccggagt ctttgatcta caaagatgct cttgttctac acaaagtcct
     5161 gcttgaaaca cgcagagacc tggagggaga tgaggactct catgtcccaa atgtgacttt
     5221 gctgattcaa gagcttatcc acaatctttt tgtgtcagtc atgagtcatc aggatgatga
     5281 gggaagatgc tacagcgatt ctttagcaga aattcctgct gtggatccca actttcctaa
     5341 caaaccaccc cttacatttg acataattag gaagaatgtt gaaaataatc gctaccgtcg
     5401 gcttgattta tttcaagagc atatgtttga agtattggaa cgagcaagaa ggatgaatcg
     5461 gacagattca gaaatatatg aagatgcagt agaacttcag cagtttttta ttaaaattcg
     5521 tgatgaactc tgcaaaaatg gagagattct tctttcaccg gcactcagct ataccacaaa
     5581 acatttgcat aatgatgtgg agaaagagag aaaggaaaaa ttgccaaaag aaatagagga
     5641 agataaacta aaacgagaag aagaaaaaag agaagctgaa aagagtgaag attcctctgg
     5701 tgctgcaggc ctctcaggct tacatcgcac atacagccag gactgtagct ttaaaaacag
     5761 catgtaccat gttggagatt acgtctatgt ggaacctgca gaggccaacc tacaaccaca
     5821 tatcgtctgt attgaaagac tgtgggagga ttcagctggt gaaaaatggt tgtatggctg
     5881 ttggttttac cgaccaaatg aaacattcca cctggctaca cgaaaatttc tagaaaaaga
     5941 agtttttaag agtgactatt acaacaaagt tccagttagt aaaattctag gcaagtgtgt
     6001 ggtcatgttt gtcaaggaat actttaagtt atgcccagaa aacttccgag atgaggatgt
     6061 ttttgtctgt gaatcacggt attctgccaa aaccaaatct tttaagaaaa ttaaactgtg
     6121 gaccatgccc atcagctcag tcaggtttgt ccctcgggat gtgcctctgc ctgtggttcg
     6181 cgtggcctct gtatttgcaa atgcagataa aggtgatgat gagaagaata cagacaactc
     6241 agaggacagt cgagctgaag acaattttaa cttggaaaag gaaaaagaag atgtccctgt
     6301 ggaaatgtcc aatggtgaac caggttgcca ctactttgag cagctccatt acaatgacat
     6361 gtggctgaag gttggcgact gtgtcttcat caagtcccat ggcctggtgc gtcctcgtgt
     6421 gggcagaatt gaaaaagtat gggttcgaga tggagctgca tatttttatg gccccatctt
     6481 cattcaccct gaagaaacag agcatgagcc cacaaaaatg ttctacaaaa aagaagtatt
     6541 tctgagtaat ctggaagaaa cctgccccat gacatgtatt ctcggaaagt gtgctgtgtt
     6601 gtcattcaag gacttcctct cctgcaggcc aactgaaata ccagaaaatg acattctgct
     6661 ttgtgagagc cgctacaatg agagcgacaa gcagatgaag aaattcaaag gattgaagag
     6721 gttttcactc tctgctaaag tggtagatga tgaaatttac tacttcagaa aaccaattgt
     6781 tcctcagaag gagccatcac ctttgctgga aaagaagatc cagttgctag aagctaaatt
     6841 tgccgagtta gaaggtggag atgatgatat tgaagagatg ggagaagaag atagtgaggt
     6901 cattgaacct ccttctctac ctcagcttca gacccccctg gccagtgagc tggacctcat
     6961 gccctacaca cccccacagt ctaccccaaa gtctgccaaa ggcagtgcaa agaaggaagg
     7021 ctccaaacgg aaaatcaaca tgagtggcta catcctgttc agcagtgaga tgagggctgt
     7081 gattaaggcc caacacccag actactcttt cggggagctc agccgcctgg tggggacaga
     7141 atggagaaat cttgagacag ccaagaaagc agaatatgaa ggcatgatgg gtggctatcc
     7201 gccaggcctt ccacctttgc agggcccagt tgatggcctt gttagcatgg gcagcatgca
     7261 gccacttcac cctggggggc ctccacccca ccatcttccg ccaggtgtgc ctggcctccc
     7321 gggcatccca ccaccgggtg tgatgaacca aggagtggcc cctatggtag ggactccagc
     7381 accgggtgga agtccatatg gacaacaggt gggagttttg gggcctccag ggcagcaggc
     7441 accacctcca tatcccggcc cacatccagc tggaccccct gtcatacagc agccaacaac
     7501 acccatgttt gtagctcccc caccaaagac ccagcggctt cttcactcag aggcctacct
     7561 gaaatacatt gaaggactca gtgcggagtc caacagcatt agcaagtggg atcagacact
     7621 ggcagctcga agacgcgacg tccatttgtc gaaagaacag gagagccgcc taccctctca
     7681 ctggctgaaa agcaaagggg cccacaccac catggcagat gccctctggc gccttcgaga
     7741 tttgatgctc cgggacaccc tcaacattcg ccaagcatac aacctagaaa atgtttaaga
     7801 attccatacc ggttaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     7861 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     7921 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     7981 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     8041 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     8101 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     8161 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     8221 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     8281 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     8341 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     8401 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     8461 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8521 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8581 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8641 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8701 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8761 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8821 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8881 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     8941 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     9001 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     9061 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9121 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9181 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9241 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9301 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9361 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9421 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9481 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9541 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     9601 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     9661 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     9721 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9781 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     9841 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     9901 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     9961 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
    10021 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
    10081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
    10261 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    10321 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    10381 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    10441 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10501 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    10561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10741 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10861 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10921 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10981 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    11041 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    11101 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    11161 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    11221 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    11281 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    11341 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
    11401 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
    11461 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
    11521 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
    11581 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
    11641 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
    11701 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
    11761 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
    11821 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    11881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    11941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    12121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    12181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    12241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    12361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    12421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    12481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    12541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    12601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    13021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    13081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    13141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    13201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    13261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    14101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    14161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    14221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    14281 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    14341 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    14401 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggt
//
