LOCUS       sequence-233686-       14459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Porter EG, Dykhuizen EC
  TITLE     Individual Bromodomains of Polybromo-1 Contribute to Chromatin 
            Association and Tumor Suppression in Clear Cell Renal Carcinoma.
  JOURNAL   J Biol Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875.
  PUBMED    28053089
REFERENCE   2  (bases 1 to 14459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Jan 4. pii: jbc.M116.746875. doi: 
            10.1074/jbc.M116.746875."
COMMENT     Sequence Label: Fuw PBRM1 mBD3
FEATURES             Location/Qualifiers
     source          1..14459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(13..31,32..54,55..73,74..96,97..115,116..138,139..157,
                     158..180,181..199,200..222,223..241,242..264,265..283)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        316..354
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     351..375
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      477..486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5407..5906
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5442..5465)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5839..5858
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5927..6526
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5927..5946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6423..6443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6550..7138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6603..6623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7021..7032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7141..7157)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7142..7158)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7663..7843
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7869..7886)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7875..8099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8145..8573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8232..8251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8442..8463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8582..8602)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8587..8916
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8767..8902
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8829..8848
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8964..9011
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9030..9404
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9534..9655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9571..9590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9625..9644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9704..9720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9704..9720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9717..9739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9728..9744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9752..9758,9759..9776,9777..9782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9797..9818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9935..9952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10106..10694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10186..10205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10865..11656,11657..11725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11488..11507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11726..11830)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11905..11924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12096..12475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12477..12675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12693..12873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12920..13045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13538..13771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13956..14000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14298..14415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
       61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
      301 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      421 cgggaccgat ccagcctccg cggccccgaa ttgctagccg atggattcgt gaattcgcca
      481 ccatgggttc caagagaaga agagctacct ccccttccag cagtgtcagc ggggactttg
      541 atgatgggca ccattctgtg tcaacaccag gcccaagcag gaaaaggagg agactttcca
      601 atcttccaac tgtagatcct attgccgtgt gccatgaact ctataatacc atccgagact
      661 ataaggatga acagggcaga cttctctgtg agctcttcat tagggcacca aagcgaagaa
      721 atcaaccaga ctattatgaa gtggtttctc agcccattga cttgatgaaa atccaacaga
      781 aactaaaaat ggaagagtat gatgatgtta atttgctgac tgctgacttc cagcttcttt
      841 ttaacaatgc aaagtcctat tataagccag attctcctga atataaagcc gcttgcaaac
      901 tctgggattt gtaccttcga acaagaaatg agtttgttca gaaaggagaa gcagatgacg
      961 aagatgatga tgaagatggg caagacaatc agggcacagt gactgaagga tcttctccag
     1021 cttacttgaa ggagatcctg gagcagcttc ttgaagccat agttgtagct acaaatccat
     1081 caggacgtct cattagcgaa ctttttcaga aactgccttc taaagtgcaa tatccagatt
     1141 attatgcaat aattaaggag cctatagatc tcaagaccat tgcccagagg atacagaatg
     1201 gaagctacaa aagtattcat gcaatggcca aagatataga tctcctcgca aaaaatgcca
     1261 aaacttataa tgagcctggc tctcaagtat tcaaggatgc aaattcaatt aaaaaaatat
     1321 tttatatgaa aaaggctgaa attgaacatc atgaaatggc taagtcaagt cttcgaatga
     1381 ggactccatc caacttggct gcagccagac tgacaggtcc ttcacacagt aaaggcagcc
     1441 ttggtgaaga gagaaatccc actagcaagt attaccgtaa taaaagagca gtacaaggag
     1501 gtcgtttatc agcaattaca atggcacttc aatatggctc agaaagtgaa gaagatgctg
     1561 ctttagctgc tgcacgctat gaagagggag agtcagaagc agaaagcatc acttccttta
     1621 tggatgtttc aaatcctttt tatcagcttt atgacacagt taggagttgt cggaataacc
     1681 aagggcagct aatagctgaa cctttttacc atttgccttc aaagaaaaaa taccctgatt
     1741 attaccagca aattaaaatg cccatatcac tacaacagat ccgaacaaaa ctgaagaatc
     1801 aagaatatga aactttagat catttggagt gtgatctgaa tttaatgttt gaaaatgcca
     1861 aacgctatgc cgtgcccaat tcagccatct acaagcgagt tctaaaattg cagcaagtta
     1921 tgcaggcaaa gaagaaagag cttgccagga gagacgatat cgaggacgga gacagcatga
     1981 tctcttcagc cacctctgat actggtagtg ccaaaagaaa aagtaaaaag aacataagaa
     2041 agcagcgaat gaaaatctta ttcaatgttg ttcttgaagc tcgagagcca ggttcaggca
     2101 gaagactttg tgacctattt atggttaaac catccaaaaa ggactatcct gattattata
     2161 aaatcatctt ggagccaatg gacttgaaaa taattgagca taacatccgc aatgacaaat
     2221 atgctggtga agagggaatg atagaagaca tgaagctgat gttccggaat gccaggcact
     2281 ataatgagga gggctcccag gtttataatg atgcacatat cctggagaag ttactcaagg
     2341 agaaaaggaa agagctgggc ccactgcctg atgatgatga catggcttct cccaaactca
     2401 agctgagtag gaagagtggc atttctccta aaaaatcaaa atacatgact ccaatgcagc
     2461 agaaactaaa tgaggtctat gaagctgtaa agaactatac tgataagagg ggtcgccgcc
     2521 tcagtgccat atttctgagg cttccctcta gatctgagtt gcctgactac tatctgacta
     2581 ttaaaaagcc catggacatg gaaaaaattc gaagtcacat gatggccaac aagtaccaag
     2641 atattgactc tatggttgag gactttgtca tgatgtttaa taatgcctgt acgtacaatg
     2701 agccggagtc tttgatctac aaagatgctc ttgttctaca caaagtcctg cttgaaacac
     2761 gcagagacct ggagggagat gaggactctc atgtcccaaa tgtgactttg ctgattcaag
     2821 agcttatcca caatcttttt gtgtcagtca tgagtcatca ggatgatgag ggaagatgct
     2881 acagcgattc tttagcagaa attcctgctg tggatcccaa ctttcctaac aaaccacccc
     2941 ttacatttga cataattagg aagaatgttg aaaataatcg ctaccgtcgg cttgatttat
     3001 ttcaagagca tatgtttgaa gtattggaac gagcaagaag gatgaatcgg acagattcag
     3061 aaatatatga agatgcagta gaacttcagc agttttttat taaaattcgt gatgaactct
     3121 gcaaaaatgg agagattctt ctttcaccgg cactcagcta taccacaaaa catttgcata
     3181 atgatgtgga gaaagagaga aaggaaaaat tgccaaaaga aatagaggaa gataaactaa
     3241 aacgagaaga agaaaaaaga gaagctgaaa agagtgaaga ttcctctggt gctgcaggcc
     3301 tctcaggctt acatcgcaca tacagccagg actgtagctt taaaaacagc atgtaccatg
     3361 ttggagatta cgtctatgtg gaacctgcag aggccaacct acaaccacat atcgtctgta
     3421 ttgaaagact gtgggaggat tcagctggtg aaaaatggtt gtatggctgt tggttttacc
     3481 gaccaaatga aacattccac ctggctacac gaaaatttct agaaaaagaa gtttttaaga
     3541 gtgactatta caacaaagtt ccagttagta aaattctagg caagtgtgtg gtcatgtttg
     3601 tcaaggaata ctttaagtta tgcccagaaa acttccgaga tgaggatgtt tttgtctgtg
     3661 aatcacggta ttctgccaaa accaaatctt ttaagaaaat taaactgtgg accatgccca
     3721 tcagctcagt caggtttgtc cctcgggatg tgcctctgcc tgtggttcgc gtggcctctg
     3781 tatttgcaaa tgcagataaa ggtgatgatg agaagaatac agacaactca gaggacagtc
     3841 gagctgaaga caattttaac ttggaaaagg aaaaagaaga tgtccctgtg gaaatgtcca
     3901 atggtgaacc aggttgccac tactttgagc agctccatta caatgacatg tggctgaagg
     3961 ttggcgactg tgtcttcatc aagtcccatg gcctggtgcg tcctcgtgtg ggcagaattg
     4021 aaaaagtatg ggttcgagat ggagctgcat atttttatgg ccccatcttc attcaccctg
     4081 aagaaacaga gcatgagccc acaaaaatgt tctacaaaaa agaagtattt ctgagtaatc
     4141 tggaagaaac ctgccccatg acatgtattc tcggaaagtg tgctgtgttg tcattcaagg
     4201 acttcctctc ctgcaggcca actgaaatac cagaaaatga cattctgctt tgtgagagcc
     4261 gctacaatga gagcgacaag cagatgaaga aattcaaagg attgaagagg ttttcactct
     4321 ctgctaaagt ggtagatgat gaaatttact acttcagaaa accaattgtt cctcagaagg
     4381 agccatcacc tttgctggaa aagaagatcc agttgctaga agctaaattt gccgagttag
     4441 aaggtggaga tgatgatatt gaagagatgg gagaagaaga tagtgaggtc attgaacctc
     4501 cttctctacc tcagcttcag acccccctgg ccagtgagct ggacctcatg ccctacacac
     4561 ccccacagtc taccccaaag tctgccaaag gcagtgcaaa gaaggaaggc tccaaacgga
     4621 aaatcaacat gagtggctac atcctgttca gcagtgagat gagggctgtg attaaggccc
     4681 aacacccaga ctactctttc ggggagctca gccgcctggt ggggacagaa tggagaaatc
     4741 ttgagacagc caagaaagca gaatatgaag gcatgatggg tggctatccg ccaggccttc
     4801 cacctttgca gggcccagtt gatggccttg ttagcatggg cagcatgcag ccacttcacc
     4861 ctggggggcc tccaccccac catcttccgc caggtgtgcc tggcctcccg ggcatcccac
     4921 caccgggtgt gatgaaccaa ggagtggccc ctatggtagg gactccagca ccgggtggaa
     4981 gtccatatgg acaacaggtg ggagttttgg ggcctccagg gcagcaggca ccacctccat
     5041 atcccggccc acatccagct ggaccccctg tcatacagca gccaacaaca cccatgtttg
     5101 tagctccccc accaaagacc cagcggcttc ttcactcaga ggcctacctg aaatacattg
     5161 aaggactcag tgcggagtcc aacagcatta gcaagtggga tcagacactg gcagctcgaa
     5221 gacgcgacgt ccatttgtcg aaagaacagg agagccgcct accctctcac tggctgaaaa
     5281 gcaaaggggc ccacaccacc atggcagatg ccctctggcg ccttcgagat ttgatgctcc
     5341 gggacaccct caacattcgc caagcataca acctagaaaa tgtttaagaa ttccataccg
     5401 gttaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     5461 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     5521 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     5581 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     5641 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5701 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5761 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5821 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5881 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     5941 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     6001 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6061 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6121 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6181 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6241 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6301 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6361 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6421 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6481 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatt cgatatcaag
     6541 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6601 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6661 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6721 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6781 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6841 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6901 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6961 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7021 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7081 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     7141 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7201 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7261 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7321 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7381 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     7441 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7501 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     7561 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     7621 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     7681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     7861 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7921 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7981 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8041 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8101 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8281 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8341 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8461 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     8581 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8641 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8701 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8761 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8821 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     8881 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8941 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     9001 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     9061 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     9121 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     9181 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     9241 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     9301 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     9361 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     9421 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9481 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9661 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    11881 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    11941 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    12001 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    12061 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
    12121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    12181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    12241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    12301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    12361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    12421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    12481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
    12541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    12601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
    12661 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
    12721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    12781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    12841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
    12901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
    12961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
    13021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
    13081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    13141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    13201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    13261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    13321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    13381 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    13441 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    13501 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    13561 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    13621 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    13681 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    13741 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    13801 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    13861 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    13921 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    13981 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    14041 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    14101 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    14161 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    14221 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
    14281 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
    14341 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
    14401 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttagatct
//
