LOCUS       sequence-23370-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Thiagalingam S, Kinzler KW, Vogelstein B
  TITLE     PAK1, a gene that can regulate p53 activity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 1995 Jun 20. 92(13):6062-6.
  PUBMED    7597081
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1995 Jun 20. 92(13):6062-6."
COMMENT     Sequence Label: RS314
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    13..120
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     29..45
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     30..46
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(80..96)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(80..96)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(128..147)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(129..147)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(154..171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(154..170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(163..185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      311..766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(398..417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggaacaaag ctggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
       61 gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc
      121 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
      181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      301 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      361 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      421 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      481 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      541 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      601 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      661 tttgatttat aaaggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      721 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
      781 ttctccttac gcatctgtgc
//
