LOCUS       sequence-233720-        8547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Septin 11 isoform II expression  (mCherry-tag at the N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Li X, Serwanski DR, Miralles CP, Nagata K, De Blas AL
  TITLE     Septin 11 is present in GABAergic synapses and plays a functional 
            role in the cytoarchitecture of neurons and GABAergic synaptic 
            connectivity.
  JOURNAL   J Biol Chem. 2009 Jun 19;284(25):17253-65. doi: 
            10.1074/jbc.M109.008870. Epub 2009 Apr 20.
  PUBMED    19380581
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.008870"; journalName: "J Biol Chem"; date: 
            "2009-06-19- 19"; volume: "284"; issue: "25"; pages: "17253-65"
COMMENT     Sequence Label: mCherry-Septin 11  isoform II
FEATURES             Location/Qualifiers
     source          1..8547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1266..1973
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1411..1429)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1679..1698
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4079..4108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4124..4141
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4164..4181)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4170..4394
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4440..4868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4527..4546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4737..4758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4877..4897)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4882..5211
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5062..5197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5124..5143
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5259..5306
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5325..5723
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5881..6002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5918..5937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5972..5991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6051..6067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6051..6067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6064..6086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6075..6091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6099..6105,6106..6123,6124..6129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6144..6165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6282..6299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6442..7030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6522..6541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7201..7992,7993..8061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7824..7843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8062..8166)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8241..8260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatcccg
     1261 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1321 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1381 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1441 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1501 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1561 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1621 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1681 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1741 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1801 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1861 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1921 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtccggac
     1981 tcagatctat ggccgtggcg gtggggagac cgagtaatga agaactgcga aacttatcac
     2041 tttctggcca tgttggattt gacagtctcc ctgaccagct ggtcaacaaa tccacttctc
     2101 aaggattctg tttcaacatc ctgtgtgtgg gagagacagg tattggcaaa tccacattga
     2161 tggacacttt attcaacacc aaatttgaaa gtgacccagc tactcacaac gaaccaggtg
     2221 tccggttaaa agccagaagt tacgaactgc aggaaagcaa cgtacggctg aagctaacca
     2281 ttgttgacac agtgggattt ggagaccaga ttaataaaga cgacagctat aagcccatag
     2341 tggagtacat tgacgcccag tttgaggcct acttgcaaga ggaattgaaa attaagcgtt
     2401 ctctcttcaa ctaccacgac acgaggattc acgcctgcct ttactttatc gcccccacgg
     2461 gacattcact gaagtccctg gacctggtca ccatgaagaa gctggacagc aaggtgaaca
     2521 tcatcccaat cattgcgaaa gccgacacca tcgccaagaa cgaattgcac aagttcaaga
     2581 gtaagatcat gagtgaactc gtgagcaacg gagtccagat atatcagttc cccaccgatg
     2641 aggaaacggt ggccgagatt aatgcaacga tgagtgtcca tctcccattt gcagtggttg
     2701 gcagtaccga agaggtgaag attggcaaca agatggcgaa ggccaggcag tacccttggg
     2761 gtgtggtgca ggttgagaac gaaaaccact gtgattttgt gaagctgagg gagatgctga
     2821 tccgagtgaa catggaggac ctgcgagagc agacgcacac tcgccactac gagttgtatc
     2881 ggcgctgtaa gctcgaggag atgggcttca aggacactga ccctgacagc aagcccttca
     2941 gtcttcagga gacatatgaa gcaaagagga atgagttcct gggagagctg cagaagaagg
     3001 aggaggagat gagacagatg tttgtcatga gagtgaagga gaaagaggcg gagctcaagg
     3061 aggcggagaa agagcttcat gagaagtttg acctcctgaa gcggactcac caagaggaga
     3121 agaaaaaggt ggaagacaag aagaaggagc ttgaggagga ggtgagcaac ttccagaaga
     3181 agaaggcggc ggctcagctc ttgcagtcgc aggcccagca gtctggggcc cagcagacca
     3241 agaaggacaa ggataagaaa aatagtccct ggctctgcac ggaataaacg atactctcaa
     3301 atctaactgg acgtgctttc gccttcgcat cttcttcttt atgtagccat cacgttgcca
     3361 agccctactc tctcttaatg gacagcatta gaagagcggg agctgtctgt tcacgagttg
     3421 gggaagagtt acctcatctc caccatcgcc tcccattctt taaagtctgg gtcaaacatt
     3481 gcactgctgt ttgtaattgt caatgtctgt ttacaaacca ccaccctcag tggctaccga
     3541 agtgacttca actaccaaac aaaataacaa taatattaag agtggaatag aaaatgtagg
     3601 cccaaacctg gggacttggg ctggaatcct cttgcttatt agctgccatt tcttgaacct
     3661 cctgatctaa gaccttatgg accgaagaga gcatgtacct cacttgaggt tgtcatcacc
     3721 tgcagggcaa acatagcaca aggtaccccc tggtctctat cctatgggca aatttcacca
     3781 accccatcag gatctaaagc tcttagcagg tcatcagagc ggggcaaccc ttgctgcctc
     3841 ctattggttt tgtgggttgt tctggtgtgg gattttagaa aaggaaaaaa aaaaaaagaa
     3901 aggtattgca tgcacagagg tgcctgcagt tttccctctg gaacgtcctg ttgatacggt
     3961 gtttttattt ttcagctgac catctgcctc ttgagagaaa gaagtgggca tccaagcttc
     4021 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     4081 acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca
     4141 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4201 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4261 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4321 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4381 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4441 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4501 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4561 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4621 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4681 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4741 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4801 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4861 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5221 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     5281 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt
     5341 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     5401 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     5461 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     5521 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     5581 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     5641 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     5701 ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg acacgtgcta
     5761 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5821 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6001 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca ggaaccgtaa
     6421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8161 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8221 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8281 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8341 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8401 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa
     8461 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     8521 ttctaggtct tgaaaggagt gggaatt
//
