LOCUS       sequence-233727-        9888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and 
            IDH1 C-terminally tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Tiburcio PDB, Xiao B, Berg S, Asper S, Lyne S, Zhang Y, Zhu X, Yan 
            H, Huang LE
  TITLE     Functional requirement of a wild-type allele for mutant IDH1 to 
            suppress anchorage-independent growth through redox homeostasis.
  JOURNAL   Acta Neuropathol. 2018 Feb;135(2):285-298. doi: 
            10.1007/s00401-017-1800-0. Epub 2017 Dec 29.
  PUBMED    29288440
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-017-1800-0"; journalName: "Acta Neuropathol"; date: 
            "2018-02"; volume: "135"; issue: "2"; pages: "285-298"
COMMENT     Sequence Label: pLenti/TO/EYFP-P2A-IDH1(HA)
FEATURES             Location/Qualifiers
     source          1..9888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2561..2563,2564..2566,2567..3277)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2606..2627)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2867..2886)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3214..3235
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3302..3322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3326..3346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3350..3370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3398..3454
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4694..4720
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4725..4749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4802..4867
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4891..5479
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4944..4964)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5362..5373)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5494..5810
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5661..5796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5858..5905
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5924..6322
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6409..6642
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6714..6835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6751..6770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6805..6824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6875..7010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7030..7049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7031..7049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7059..7076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7059..7075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7068..7090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7217..7672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7304..7323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7514..7535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7698..7802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7803..7871,7872..8663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8021..8040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8834..9422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9323..9342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9576..9593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9710..9731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9746..9751,9752..9769,9770..9776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9784..9800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9789..9811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9808..9824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9808..9824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9843..9863
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9845..9863
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctcacc atggtgagca agggcgagga
     2581 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2641 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2701 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     2761 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2821 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2881 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2941 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3001 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3061 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3121 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     3181 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3241 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctga
     3301 tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa
     3361 gagaaaggta ggatccaccg gatctagagg aagcggagct actaacttca gcctgctgaa
     3421 gcaggctgga gacgtggagg agaaccctgg accttccaaa aaaatcagtg gcggttctgt
     3481 ggtagagatg caaggagatg aaatgacacg aatcatttgg gaattgatta aagagaaact
     3541 catttttccc tacgtggaat tggatctaca tagctatgat ttaggcatag agaatcgtga
     3601 tgccaccaac gaccaagtca ccaaggatgc tgcagaagct ataaagaagc ataatgttgg
     3661 cgtcaaatgt gccactatca ctcctgatga gaagagggtt gaggagttca agttgaaaca
     3721 aatgtggaaa tcaccaaatg gcaccatacg aaatattctg ggtggcacgg tcttcagaga
     3781 agccattatc tgcaaaaata tcccccggct tgtgagtgga tgggtaaaac ctatcatcat
     3841 aggtcgtcat gcttatgggg atcaatacag agcaactgat tttgttgttc ctgggcctgg
     3901 aaaagtagag ataacctaca caccaagtga cggaacccaa aaggtgacat acctggtaca
     3961 taactttgaa gaaggtggtg gtgttgccat ggggatgtat aatcaagata agtcaattga
     4021 agattttgca cacagttcct tccaaatggc tctgtctaag ggttggcctt tgtatctgag
     4081 caccaaaaac actattctga agaaatatga tgggcgtttt aaagacatct ttcaggagat
     4141 atatgacaag cagtacaagt cccagtttga agctcaaaag atctggtatg agcataggct
     4201 catcgacgac atggtggccc aagctatgaa atcagaggga ggcttcatct gggcctgtaa
     4261 aaactatgat ggtgacgtgc agtcggactc tgtggcccaa gggtatggct ctctcggcat
     4321 gatgaccagc gtgctggttt gtccagatgg caagacagta gaagcagagg ctgcccacgg
     4381 gactgtaacc cgtcactacc gcatgtacca gaaaggacag gagacgtcca ccaatcccat
     4441 tgcttccatt tttgcctgga ccagagggtt agcccacaga gcaaagcttg ataacaataa
     4501 agagcttgcc ttctttgcaa atgctttgga agaagtctct attgagacaa ttgaggctgg
     4561 cttcatgacc aaggacttgg ctgcttgcat taaaggttta cccaatgtgc aacgttctga
     4621 ctacttgaat acatttgagt tcatggataa acttggagaa aacttgaaga tcaaactagc
     4681 tcaggccaaa ctttacccat acgacgtgcc tgactacgcc taggacccag ctttcttgta
     4741 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagaggggc cgcggttcga
     4801 agactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga
     4861 tgacaagcgt accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa
     4921 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4981 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5041 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5101 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5161 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5281 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5341 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5401 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5461 ctttgggccg cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag
     5521 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5641 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5701 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5761 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5821 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     5881 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
     5941 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     6001 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     6061 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     6121 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     6181 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     6241 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     6301 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
     6361 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6421 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6661 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6841 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6961 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     7021 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7201 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9781 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9841 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
