LOCUS       sequence-23374-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss 
            MJ, Moon RT
  TITLE     The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the 
            Wnt-beta-catenin pathway by targeting Dishevelled for degradation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):348-57.
  PUBMED    16547521
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):348-57."
COMMENT     Sequence Label: XE252 pCDNA3.1(zeo)-hDsh3-333-716
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc
      121 ggatccgcca ccatggacta caaggacgac gacgacaagg gcggcgcgcc agtagccaag
      181 tgctgggacc caagtccacg tggttgcttc acattgccca ggagcgagcc catccggccc
      241 attgaccctg cggcctgggt ctcccacact gcagccatga ccggcacctt ccctgcatac
      301 ggcatgagcc cctccctgag caccatcacc tccaccagct cctccatcac cagttccatc
      361 cctgacacag agcgcctaga cgacttccac ttgtccatcc acagtgacat ggctgccatc
      421 gtaaaagcca tggcctcccc tgaatcaggg ttggaggtcc gtgaccgcat gtggctcaag
      481 attaccatcc ctaatgcttt catcggctca gatgtggtgg actggctgta ccacaatgtg
      541 gaaggcttca cggaccggag ggaggcccgc aagtatgcca gcaacctgct gaaagctggc
      601 ttcatccgcc ataccgtcaa caagatcacc ttctccgagc agtgctacta catcttcggt
      661 gacctctgcg gcaacatggc caacctgtct ctccacgatc acgatggctc cagtggcgcc
      721 tctgaccagg acacactggc ccctttgccg cacccggggg ccgccccttg gcccatggct
      781 ttcccgtacc agtaccggcc anccccgcac ccata
//
