LOCUS       sequence-233740-        8008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-cas9 targeting in E. coli with ampicillin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pJZ002
FEATURES             Location/Qualifiers
     source          1..8008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(104..182)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             478..4584
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             478..4581
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4605..4736
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4737..4772
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4803..4838
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     CDS             complement(5145..6095)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6143..6365
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(join(6987..7778,7779..7847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7610..7629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7848..7952)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaggattaa gagattaatt tccctaaaaa tgataaaaca agcgttttga aagcgcttgt
       61 ttttttggtt tgcagtcaga gtagaataga agtatcaaaa aaagcaccga ctcggtgcca
      121 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatgctgtt ttgaatggtt
      181 ccaacaagat tattttataa cttttataac aaataatcaa ggagaaattc aaagaaattt
      241 atcagccata aaacaatact taatactata gaatgataac aaaataaact actttttaaa
      301 agaattttgt gttataatct atttattatt aagtattggg taatattttt tgaagagata
      361 ttttgaaaaa gaaaaattaa agcatattaa actaatttcg gaggtcatta aaactattat
      421 tgaaatcatc aaactcatta tggatttaat ttaaactttt tattttagga ggcaaaaatg
      481 gataagaaat actcaatagg cttagatatc ggcacaaata gcgtcggatg ggcggtgatc
      541 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
      601 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
      661 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
      721 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
      781 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat
      841 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
      901 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
      961 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
     1021 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
     1081 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
     1141 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
     1201 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
     1261 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
     1321 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
     1381 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
     1441 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     1501 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     1561 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     1621 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     1681 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     1741 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa
     1801 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     1861 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     1921 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     1981 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     2041 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     2101 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     2161 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     2221 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     2281 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     2341 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     2401 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     2461 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     2521 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     2581 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     2641 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     2701 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     2761 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     2821 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     2881 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     2941 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt
     3001 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     3061 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     3121 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     3181 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     3241 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     3301 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     3361 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     3421 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     3481 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     3541 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     3601 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     3661 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     3721 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     3781 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     3841 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     3901 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     3961 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     4021 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     4081 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     4141 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     4201 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     4261 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     4321 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     4381 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     4441 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     4501 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     4561 ttgagtcagc taggaggtga ctgaagtata ttttagatga agattatttc ttaataacta
     4621 aaaatatggt ataatactct taataaatgc agtaatacag gggcttttca agactgaagt
     4681 ctagctgaga caaatagtgc gattacgaaa ttttttagac aaaaatagtc tacgaggttt
     4741 tagagctatg ctgttttgaa tggtcccaaa actgagacca gtctcggaag ctcaaaggtc
     4801 tcgttttaga gctatgctgt tttgaatggt cccaaaactt cagcacactg agacttgttg
     4861 agttccatgt tttagagcta tgctgttttg aatggactcc attcaacatt gccgatgata
     4921 acttgagaaa gagggttaat accagcagtc ggataccttc ctattctttc tgttaaagcg
     4981 ttttcatgtt ataataggca aaagaagagt agtgtgatcg tccattccta agctagccca
     5041 tgggtatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg
     5101 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     5161 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     5221 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     5281 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     5341 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     5401 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     5461 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     5521 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     5581 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     5641 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     5701 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     5761 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     5821 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     5881 ttagctaata caccataagc attttcccta ctgatgttca tcatctgaac gtattggtta
     5941 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     6001 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     6061 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     6121 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     6181 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     6241 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     6301 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     6361 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     6421 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcggtt
     6481 gcggccacaa tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     6541 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     6601 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     6661 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     6721 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     6781 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaacg
     6841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca
     7141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7861 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7921 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     7981 gatggcagcg cctaaatgta gaatgata
//
