LOCUS       sequence-233748-       11589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries promoter cassette containing ermE*p promoter and ampicillin 
            resistance gene that can be amplified with homology sequence for 
            refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-ermE*p
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(147..158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    229..328
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             338..2155
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             join(3121..3189,3190..3981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3339..3358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             4137..4937
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    5015..5518
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5559..5577
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5615..5637)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5737..5756
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5777..6057
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6058..6732
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(6972..6993)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      7240..7828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7729..7748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7982..7999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8213..8347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8237..8256)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8291..8310
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8772..8792)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8922..8987
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(9407..10201)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9518..9537)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10128..10147
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(10985..11356)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11389..11498
                     /note="incP origin of transfer"
ORIGIN
        1 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag
       61 gcatgcaagc tctagcgatt ccagacgtcc cgaaggcgtg gcgcggcttc cccgtgccgg
      121 agcaatcgcc ctgggtgggt tacacgacgc ccctctatgg cccgtactga cggacacacc
      181 gaagccccgg cggcaaccct cagcggatgc cccggggctt cacgttttcc caggtcagaa
      241 gcggttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
      301 tagggtcgcc gacatgacac aaggggttgt gaccggggtg gacacgtacg cgggtgctta
      361 cgaccgtcag tcgcgcgagc gcgaaaattc gagcgcagca agcccagcga cacagcgtag
      421 cgccaacgaa gacaaggcgg ccgaccttca gcgcgaagtc gagcgcgacg ggggccggtt
      481 caggttcgtc gggcatttca gcgaagcgcc gggcacgtcg gcgttcggga cggcggagcg
      541 cccggagttc gaacgcatcc tgaacgaatg ccgcgccggg cggctcaaca tgatcattgt
      601 ctatgacgtg tcgcgcttct cgcgcctgaa ggtcatggac gcgattccga ttgtctcgga
      661 attgctcgcc ctgggcgtga cgattgtttc cactcaggaa ggcgtcttcc ggcagggaaa
      721 cgtcatggac ctgattcacc tgattatgcg gctcgacgcg tcgcacaaag aatcttcgct
      781 gaagtcggcg aagattctcg acacgaagaa ccttcagcgc gaattgggcg ggtacgtcgg
      841 cgggaaggcg ccttacggct tcgagcttgt ttcggagacg aaggagatca cgcgcaacgg
      901 ccgaatggtc aatgtcgtca tcaacaagct tgcgcactcg accactcccc ttaccggacc
      961 cttcgagttc gagcccgacg taatccggtg gtggtggcgt gagatcaaga cgcacaaaca
     1021 ccttcccttc aagccgggca gtcaagccgc cattcacccg ggcagcatca cggggctttg
     1081 taagcgcatg gacgctgacg ccgtgccgac ccggggcgag acgattggga agaagaccgc
     1141 ttcaagcgcc tgggacccgg caaccgttat gcgaatcctt cgggccccgc gtattgcggg
     1201 cttcgccgct gaggtgatct acaagaagaa gccggacggc acgccgacca cgaagattga
     1261 gggttaccgc attcagcgcg acccgatcac gctccggccg gtcgagcttg attgcggacc
     1321 gatcatcgag cccgctgagt ggtatgagct tcaggcgtgg ttggacggca gggggcgcgg
     1381 caaggggctt tcccgggggc aagccattct gtccgccatg gacaagctgt actgcgagtg
     1441 tggcgccgtc atgacttcga agcgcgggga agaatcgatc aaggactctt accgctgccg
     1501 tcgccggaag gtggtcgacc cgtccgcacc tgggcagcac gaaggcacgt gcaacgtcag
     1561 catggcggca ctcgacaagt tcgttgcgga acgcatcttc aacaagatca ggcacgccga
     1621 aggcgacgaa gagacgttgg cgcttctgtg ggaagccgcc cgacgcttcg gcaagctcac
     1681 tgaggcgcct gagaagagcg gcgaacgggc gaaccttgtt gcggagcgcg ccgacgccct
     1741 gaacgccctt gaagagctgt acgaagaccg cgcggcaggc gcgtacgacg gacccgttgg
     1801 caggaagcac ttccggaagc aacaggcagc gctgacgctc cggcagcaag gggcggaaga
     1861 gcggcttgcc gaacttgaag ccgccgaagc cccgaagctt ccccttgacc aatggttccc
     1921 cgaagacgcc gacgctgacc cgaccggccc taagtcgtgg tgggggcgcg cgtcagtaga
     1981 cgacaagcgc gtgttcgtcg ggctcttcgt agacaagatc gttgtcacga agtcgactac
     2041 gggcaggggg cagggaacgc ccatcgagaa gcgcgcttcg atcacgtggg cgaagccgcc
     2101 gaccgacgac gacgaagacg acgcccagga cggcacggaa gacgtagcgg cgtagcgaga
     2161 cacccgggaa gcctgatcta cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     2221 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     2281 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgact
     2341 agtcctacaa caactaagaa aatggctatc atgcggaagc tggtgagaag aacagcatcg
     2401 ggacaaggga aggaagaaca aagacaaaga aaacaaaaga aagcaattga aaacaaaaca
     2461 aaacaatttt cattccttct cttatcattc cttttctttt cttttctctc attcaacgca
     2521 ctccatcgta tccgtattcc tcttattttt tctctttctc tatatccatt tctttctctc
     2581 taggtgtgtc ctctctctct cttcaatttc tctactccgc attccaacgc atccttcccc
     2641 caacctccca tttcctcctt acggcccgat agcgatcgtc tttccctcgc tatcactcgc
     2701 taccggcccc tcctctgcac cgtaacctcc tacgtattta ccatatcata aagttttttc
     2761 cgacgcttat cgctgacccc ctgccgccct cctattggct tccggattat cttcttgtcc
     2821 ataaggtgat ccatgcttcc tgaagattcc cgaaatgtgt ccactttggc ggggaatcat
     2881 tccatccact tctttctctc tcgctttcct cattcggcgc tccccttccg cgtctcattg
     2941 gtcttccgct ccgtttttgc tttgccgatg ttacttgggg agaggtgcga taatcctttc
     3001 gcaaaaactc ggtttgacgc ctcccatggt ataaatagtg gctcgagcat tcaaatatgt
     3061 atccgctcat gagacaataa ccctgataaa tgcttcaatc atgattgaaa aaggaagagt
     3121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3181 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3241 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3301 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3361 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3421 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3481 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3541 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3601 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3661 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3721 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3781 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3841 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3901 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3961 tcactgatta agcattggta acatatgtgg tgcacgcggt cgatcttgac ggctggcgag
     4021 aggtgcgggg aggatctgac cgacgcggtc cacacgtggc accgcgatgc tgttgtgggc
     4081 acaatcgtgc cggttggtag gatccagcgg tccaacgcac cccaggaggt ccccattcga
     4141 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     4201 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     4261 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     4321 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     4381 agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
     4441 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     4501 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcggaagaa gtaacaaagg
     4561 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta gctactggag
     4621 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
     4681 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
     4741 gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
     4801 tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
     4861 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
     4921 gcggccagca aaactaaggt acctgtattt agaaaaataa acaaataggg gttccgcgca
     4981 catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa
     5041 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
     5101 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
     5161 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
     5221 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
     5281 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
     5341 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
     5401 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
     5461 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
     5521 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5581 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5641 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5701 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     5761 gagtgcacca tagatcacga cattactata tatataatat aggaagcatt taatagaaca
     5821 gcatcgtaat atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt
     5881 ctttattgaa aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt
     5941 tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat
     6001 tggtgactat tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg
     6061 tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc
     6121 acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg
     6181 cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa
     6241 gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct
     6301 aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga
     6361 gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta
     6421 tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt
     6481 cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct
     6541 gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg
     6601 acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc
     6661 ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat
     6721 gctaagaaat aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccga
     6781 tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     6841 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     6901 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     6961 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7021 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaacctaac tgtcagacca
     7081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7981 cagcgagtca gtgagcgagg aagcggaaga gcgctgactt ccgcgtttcc agactttacg
     8041 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     8101 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     8161 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt cagatccaga
     8221 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     8281 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     8341 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     8401 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc
     8461 aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta
     8521 cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa
     8581 aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga atatttttcc
     8641 ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca
     8701 agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc
     8761 ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta
     8821 gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca
     8881 ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt
     8941 agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa
     9001 atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag
     9061 atccggacca aagcggccat cgtgcctccc cactcctgca gttcgggggc atggatgcgc
     9121 ggatagccgc tgctggtttc ctggatgccg acggatttgc actgccggta gaactccgcg
     9181 aggtcgtcca gcctcaggca gcagctgaac caactcgcga ggggatcgag cccggggtgg
     9241 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     9301 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     9361 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     9421 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     9481 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     9541 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     9601 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     9661 cgtcgggcat ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     9721 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     9781 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     9841 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     9901 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     9961 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
    10021 ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
    10081 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
    10141 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
    10201 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
    10261 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
    10321 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
    10381 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
    10441 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
    10501 tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gagagctcat
    10561 gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgt
    10621 ggagcggatc ggggattgtc tttcttcagc tcgctgatga tatgctgacg ctcaatgccg
    10681 tttggcctcc gactaacgaa aatcccgcat ttggacggct gatccgattg gcacggcgga
    10741 cggcgaatgg cggagcagac gctcgtccgg gggcaatgag atatgaaaaa gcctgaactc
    10801 accgcgacgt atcgggccct ggccagctag ctagagtcga cctgcaggtc cccggggatc
    10861 ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc tcttcttgat
    10921 ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt agcggctaaa
    10981 aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct
    11041 gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc
    11101 gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg tcgccattga
    11161 tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg tagccctggc
    11221 cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt tcctcaatcg
    11281 ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct
    11341 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
    11401 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
    11461 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
    11521 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
    11581 ataccgaaa
//
