LOCUS       sequence-233750-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries bidirectional promoter cassette containing sp44 and p21 
            promoters and apramycin resistance gene flanked by two FRT sites 
            that can be amplified with homology sequence for refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-sp44/p21
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    71..170
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             180..1997
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    2992..3025
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             3119..3919
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     protein_bind    3923..3956
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             4041..4844
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    4922..5425
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5466..5484
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5522..5544)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5644..5663
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5684..5964
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5965..6639
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(6879..6900)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      7147..7735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7636..7655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7889..7906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8120..8254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8144..8163)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8198..8217
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8679..8699)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8829..8894
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(9314..10108)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9425..9444)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10035..10054
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(10892..11263)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11296..11405
                     /note="incP origin of transfer"
     CDS             complement(11643..11654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggcccgtact gacggacaca ccgaagcccc ggcggcaacc ctcagcggat gccccggggc
       61 ttcacgtttt cccaggtcag aagcggtttt cgggagtagt gccccaactg gggtaacctt
      121 tgagttctct cagttggggg cgtagggtcg ccgacatgac acaaggggtt gtgaccgggg
      181 tggacacgta cgcgggtgct tacgaccgtc agtcgcgcga gcgcgaaaat tcgagcgcag
      241 caagcccagc gacacagcgt agcgccaacg aagacaaggc ggccgacctt cagcgcgaag
      301 tcgagcgcga cgggggccgg ttcaggttcg tcgggcattt cagcgaagcg ccgggcacgt
      361 cggcgttcgg gacggcggag cgcccggagt tcgaacgcat cctgaacgaa tgccgcgccg
      421 ggcggctcaa catgatcatt gtctatgacg tgtcgcgctt ctcgcgcctg aaggtcatgg
      481 acgcgattcc gattgtctcg gaattgctcg ccctgggcgt gacgattgtt tccactcagg
      541 aaggcgtctt ccggcaggga aacgtcatgg acctgattca cctgattatg cggctcgacg
      601 cgtcgcacaa agaatcttcg ctgaagtcgg cgaagattct cgacacgaag aaccttcagc
      661 gcgaattggg cgggtacgtc ggcgggaagg cgccttacgg cttcgagctt gtttcggaga
      721 cgaaggagat cacgcgcaac ggccgaatgg tcaatgtcgt catcaacaag cttgcgcact
      781 cgaccactcc ccttaccgga cccttcgagt tcgagcccga cgtaatccgg tggtggtggc
      841 gtgagatcaa gacgcacaaa caccttccct tcaagccggg cagtcaagcc gccattcacc
      901 cgggcagcat cacggggctt tgtaagcgca tggacgctga cgccgtgccg acccggggcg
      961 agacgattgg gaagaagacc gcttcaagcg cctgggaccc ggcaaccgtt atgcgaatcc
     1021 ttcgggcccc gcgtattgcg ggcttcgccg ctgaggtgat ctacaagaag aagccggacg
     1081 gcacgccgac cacgaagatt gagggttacc gcattcagcg cgacccgatc acgctccggc
     1141 cggtcgagct tgattgcgga ccgatcatcg agcccgctga gtggtatgag cttcaggcgt
     1201 ggttggacgg cagggggcgc ggcaaggggc tttcccgggg gcaagccatt ctgtccgcca
     1261 tggacaagct gtactgcgag tgtggcgccg tcatgacttc gaagcgcggg gaagaatcga
     1321 tcaaggactc ttaccgctgc cgtcgccgga aggtggtcga cccgtccgca cctgggcagc
     1381 acgaaggcac gtgcaacgtc agcatggcgg cactcgacaa gttcgttgcg gaacgcatct
     1441 tcaacaagat caggcacgcc gaaggcgacg aagagacgtt ggcgcttctg tgggaagccg
     1501 cccgacgctt cggcaagctc actgaggcgc ctgagaagag cggcgaacgg gcgaaccttg
     1561 ttgcggagcg cgccgacgcc ctgaacgccc ttgaagagct gtacgaagac cgcgcggcag
     1621 gcgcgtacga cggacccgtt ggcaggaagc acttccggaa gcaacaggca gcgctgacgc
     1681 tccggcagca aggggcggaa gagcggcttg ccgaacttga agccgccgaa gccccgaagc
     1741 ttccccttga ccaatggttc cccgaagacg ccgacgctga cccgaccggc cctaagtcgt
     1801 ggtgggggcg cgcgtcagta gacgacaagc gcgtgttcgt cgggctcttc gtagacaaga
     1861 tcgttgtcac gaagtcgact acgggcaggg ggcagggaac gcccatcgag aagcgcgctt
     1921 cgatcacgtg ggcgaagccg ccgaccgacg acgacgaaga cgacgcccag gacggcacgg
     1981 aagacgtagc ggcgtagcga gacacccggg aagcctgatc tacgtctgtc gagaagtttc
     2041 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     2101 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     2161 atggtttcta caaagatcga ctagtcctac aacaactaag aaaatggcta tcatgcggaa
     2221 gctggtgaga agaacagcat cgggacaagg gaaggaagaa caaagacaaa gaaaacaaaa
     2281 gaaagcaatt gaaaacaaaa caaaacaatt ttcattcctt ctcttatcat tccttttctt
     2341 ttcttttctc tcattcaacg cactccatcg tatccgtatt cctcttattt tttctctttc
     2401 tctatatcca tttctttctc tctaggtgtg tcctctctct ctcttcaatt tctctactcc
     2461 gcattccaac gcatccttcc cccaacctcc catttcctcc ttacggcccg atagcgatcg
     2521 tctttccctc gctatcactc gctaccggcc cctcctctgc accgtaacct cctacgtatt
     2581 taccatatca taaagttttt tccgacgctt atcgctgacc ccctgccgcc ctcctattgg
     2641 cttccggatt atcttcttgt ccataaggtg atccatgctt cctgaagatt cccgaaatgt
     2701 gtccactttg gcggggaatc attccatcca cttctttctc tctcgctttc ctcattcggc
     2761 gctccccttc cgcgtctcat tggtcttccg ctccgttttt gctttgccga tgttacttgg
     2821 ggagaggtgc gataatcctt tcgcaaaaac tcggtttgac gcctcccatg gtataaatag
     2881 tggccatatg ggtgaaccga tctcctcgtt agggtcacac cagactttac aacaccgcac
     2941 agcatgttgt caaagcagag acggttcgaa tgtgaacagc cactgcagtt cgaagttcct
     3001 attctctaga aagtatagga acttcggttc atgtgcagct ccatcagcaa aaggggatga
     3061 taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg atcgactgat
     3121 gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg
     3181 tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca gctccttccg
     3241 tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc gtgctgcgct
     3301 gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg agccgttcga
     3361 tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat tctggcgcct
     3421 gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc cacaggcaga
     3481 gcagatcatc tctgatccat tgcccctgcc acctcactcg cctgcaagcc cggtcgcccg
     3541 tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg ccaacacgac
     3601 gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac actgcaccat
     3661 tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact gctgtgagcg
     3721 ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag gtccagtcgg
     3781 tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc tgggtcaact
     3841 gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc
     3901 cgctcgccag tcgattggct gagaagttcc tattctctag aaagtatagg aacttcaagc
     3961 tttgctcgag tgtgcgggct ctaacacgtc ctagtatggt aggatgagca atctagtcga
     4021 gcaacggagg tacggaccat atgtcgaaag ctacatataa ggaacgtgct gctactcatc
     4081 ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg
     4141 cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca
     4201 aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag
     4261 ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat
     4321 ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag
     4381 aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga
     4441 agcaggcggc ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt
     4501 catgcaaggg ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga
     4561 gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag
     4621 gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg
     4681 gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg
     4741 gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag
     4801 caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaggtacc tgtatttaga
     4861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt
     4921 catcacgtgc tataaaaata attataattt aaatttttta atataaatat ataaattaaa
     4981 aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa
     5041 gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta
     5101 ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt
     5161 ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat
     5221 aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt
     5281 tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca
     5341 taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc
     5401 gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg acattaacct
     5461 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5521 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5581 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5641 atgcggcatc agagcagatt gtactgagag tgcaccatag atcacgacat tactatatat
     5701 ataatatagg aagcatttaa tagaacagca tcgtaatata tgtgtacttt gcagttatga
     5761 cgccagatgg cagtagtgga agatattctt tattgaaaaa tagcttgtca ccttacgtac
     5821 aatcttgatc cggagctttt ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga
     5881 aaaggagagg gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt
     5941 aagcacacaa aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca
     6001 ttggtgaaag tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat
     6061 gctgacttgc tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt
     6121 gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac
     6181 ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac
     6241 ggcattgata tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc
     6301 ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca
     6361 gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt
     6421 gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc
     6481 ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta
     6541 aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct
     6601 aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta
     6661 tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg aaataccgca cagatgcgta
     6721 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     6781 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6841 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6901 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6961 cactacgtga acctaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7921 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     7981 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     8041 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     8101 cgatcatgcg cacccgtcag atccagacat gataagatac attgatgagt ttggacaaac
     8161 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     8221 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     8281 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     8341 tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc cttattaacc
     8401 cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt aatagcagac
     8461 actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact gttatgccta
     8521 cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc agctttttcc
     8581 tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa
     8641 aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga
     8701 ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag
     8761 caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc agttccatag
     8821 gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta tacacttaaa
     8881 aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca
     8941 ccacagaagt aaggttcctt cacaaagatc cggaccaaag cggccatcgt gcctccccac
     9001 tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc tggtttcctg gatgccgacg
     9061 gatttgcact gccggtagaa ctccgcgagg tcgtccagcc tcaggcagca gctgaaccaa
     9121 ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc cccgcgctgg
     9181 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     9241 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     9301 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     9361 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     9421 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     9481 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     9541 cgccatgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc ctggcgaaca
     9601 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     9661 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     9721 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     9781 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     9841 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     9901 gccacgatag ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac aggtcggtct
     9961 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
    10021 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
    10081 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag
    10141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
    10201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
    10261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
    10321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
    10381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
    10441 agtgcttgcg gcagcgtgag agctcatgag cggagaacga gatgacgttg gaggggcaag
    10501 gtcgcgctga ttgctggggc aacacgtgga gcggatcggg gattgtcttt cttcagctcg
    10561 ctgatgatat gctgacgctc aatgccgttt ggcctccgac taacgaaaat cccgcatttg
    10621 gacggctgat ccgattggca cggcggacgg cgaatggcgg agcagacgct cgtccggggg
    10681 caatgagata tgaaaaagcc tgaactcacc gcgacgtatc gggccctggc cagctagcta
    10741 gagtcgacct gcaggtcccc ggggatcggt cttgccttgc tcgtcggtga tgtacttcac
    10801 cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg
    10861 caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc
    10921 ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga
    10981 tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc
    11041 cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca
    11101 cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc
    11161 cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga
    11221 taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
    11281 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
    11341 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
    11401 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
    11461 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
    11521 agggttatgc agcggaaaag atccgtcgac ctgcaggcat gcaagctcta gcgattccag
    11581 acgtcccgaa ggcgtggcgc ggcttccccg tgccggagca atcgccctgg gtgggttaca
    11641 cgacgcccct ctat
//
