LOCUS       sequence-233753-       12568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries promoter cassette containing act1p promoter and apramycin 
            resistance gene that can be amplified with homology sequence for 
            refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-act1p
FEATURES             Location/Qualifiers
     source          1..12568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             95..1912
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2898..3698
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     misc_feature    5751..6254
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6295..6313
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6351..6373)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6473..6492
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6513..6793
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6794..7468
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(7708..7729)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      7976..8564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8465..8484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8718..8735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8949..9083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8973..8992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9027..9046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9508..9528)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9658..9723
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(10143..10937)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10254..10273)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10864..10883
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(11721..12092)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            12125..12234
                     /note="incP origin of transfer"
     CDS             complement(12472..12483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(12554..12568,1..85)
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag
       61 ggtcgccgac atgacacaag gggttgtgac cggggtggac acgtacgcgg gtgcttacga
      121 ccgtcagtcg cgcgagcgcg aaaattcgag cgcagcaagc ccagcgacac agcgtagcgc
      181 caacgaagac aaggcggccg accttcagcg cgaagtcgag cgcgacgggg gccggttcag
      241 gttcgtcggg catttcagcg aagcgccggg cacgtcggcg ttcgggacgg cggagcgccc
      301 ggagttcgaa cgcatcctga acgaatgccg cgccgggcgg ctcaacatga tcattgtcta
      361 tgacgtgtcg cgcttctcgc gcctgaaggt catggacgcg attccgattg tctcggaatt
      421 gctcgccctg ggcgtgacga ttgtttccac tcaggaaggc gtcttccggc agggaaacgt
      481 catggacctg attcacctga ttatgcggct cgacgcgtcg cacaaagaat cttcgctgaa
      541 gtcggcgaag attctcgaca cgaagaacct tcagcgcgaa ttgggcgggt acgtcggcgg
      601 gaaggcgcct tacggcttcg agcttgtttc ggagacgaag gagatcacgc gcaacggccg
      661 aatggtcaat gtcgtcatca acaagcttgc gcactcgacc actcccctta ccggaccctt
      721 cgagttcgag cccgacgtaa tccggtggtg gtggcgtgag atcaagacgc acaaacacct
      781 tcccttcaag ccgggcagtc aagccgccat tcacccgggc agcatcacgg ggctttgtaa
      841 gcgcatggac gctgacgccg tgccgacccg gggcgagacg attgggaaga agaccgcttc
      901 aagcgcctgg gacccggcaa ccgttatgcg aatccttcgg gccccgcgta ttgcgggctt
      961 cgccgctgag gtgatctaca agaagaagcc ggacggcacg ccgaccacga agattgaggg
     1021 ttaccgcatt cagcgcgacc cgatcacgct ccggccggtc gagcttgatt gcggaccgat
     1081 catcgagccc gctgagtggt atgagcttca ggcgtggttg gacggcaggg ggcgcggcaa
     1141 ggggctttcc cgggggcaag ccattctgtc cgccatggac aagctgtact gcgagtgtgg
     1201 cgccgtcatg acttcgaagc gcggggaaga atcgatcaag gactcttacc gctgccgtcg
     1261 ccggaaggtg gtcgacccgt ccgcacctgg gcagcacgaa ggcacgtgca acgtcagcat
     1321 ggcggcactc gacaagttcg ttgcggaacg catcttcaac aagatcaggc acgccgaagg
     1381 cgacgaagag acgttggcgc ttctgtggga agccgcccga cgcttcggca agctcactga
     1441 ggcgcctgag aagagcggcg aacgggcgaa ccttgttgcg gagcgcgccg acgccctgaa
     1501 cgcccttgaa gagctgtacg aagaccgcgc ggcaggcgcg tacgacggac ccgttggcag
     1561 gaagcacttc cggaagcaac aggcagcgct gacgctccgg cagcaagggg cggaagagcg
     1621 gcttgccgaa cttgaagccg ccgaagcccc gaagcttccc cttgaccaat ggttccccga
     1681 agacgccgac gctgacccga ccggccctaa gtcgtggtgg gggcgcgcgt cagtagacga
     1741 caagcgcgtg ttcgtcgggc tcttcgtaga caagatcgtt gtcacgaagt cgactacggg
     1801 cagggggcag ggaacgccca tcgagaagcg cgcttcgatc acgtgggcga agccgccgac
     1861 cgacgacgac gaagacgacg cccaggacgg cacggaagac gtagcggcgt agcgagacac
     1921 ccgggaagcc tgatctacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     1981 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     2041 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgactagt
     2101 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
     2161 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
     2221 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
     2281 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
     2341 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
     2401 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
     2461 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
     2521 cgcttatcgc tgaccccctg ccgccctcct attggcttcc ggattatctt cttgtccata
     2581 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
     2641 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
     2701 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
     2761 aaaactcggt ttgacgcctc ccatggtata aatagtggct cgagggttca tgtgcagctc
     2821 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2881 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2941 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     3001 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     3061 aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     3121 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     3181 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     3241 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca cctcactcgc
     3301 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     3361 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     3421 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     3481 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     3541 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3601 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3661 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg acatatgcca ctgcctctcg
     3721 gtaaaatcca gcaaaaatta atcagtgcag ctcgctgcac tgattaattt ttgatcaata
     3781 ggagatcgct tgtgacggca agcacattga aatctgttga gtaggcctgt tattgtcgcc
     3841 cccaggagac ggagaatctc gacgggggcg cagatgagat tcaacttatt gggacgtgtc
     3901 catgtaatca ccgatgcggg atgtgtaatt ccgcttaaat cctcgaaggc gacccagctc
     3961 ctggtgctgc tgctcctcag gcggcacgag gtggtgggat cgggggtgct catcgaggag
     4021 ttgtgggcgg accacccgcc ccgcagcgcc atgacgacgc tgcagacgta cgtgtaccac
     4081 acccgccggc tgctggggga gcaccgggtg acgagcgacg accgggaatt ggtcctgacc
     4141 cagccgcccg gctacttcgc cctgatcgac gaggacgaac tcgacgtcgc ggtcgccgag
     4201 cgtctgatcc gcaccggcgg ccggctgctc gaggagaacc ggctcgagga ggcgctcgcc
     4261 tcgttggacg cgggactgga tctctggcga ggcccggcgc tgtccaccgt accgtgcggc
     4321 cgggtgctcg aaagcaatat cgcgcacctg gaagagctgc ggctttttgg aatgcagctc
     4381 cgtatcgacg cgaattggcg gctgggcaga atagggccga tgattccgga actccggtcc
     4441 ctggtaattt cgcatccgct gaacgagacc ctgcacgcca aactgatggg cgcgctctgt
     4501 cagatgggca ggcgcgccga ggcgctggaa tcgtatcgga atctccggcg gatactgtcc
     4561 gacgaactgg gggtggatcc gacgccggaa atccagcgta tgcacatgga aattctcaac
     4621 ggtgagaagg tgctcgtgta gcaccggtcc gtgaacgcgg tggagcccta tgtctcttaa
     4681 gtgttcccct ccctgcctcg tggtccctca cgcgctcagc tttgggcgcc cggctcgagc
     4741 ggcggtcgaa gggagatggg gtgccgctgg acgcggcgcc ggtggatccg gcatcgaggg
     4801 gtcccgtatc ggccttcgag cctccttcga gccacggggc cgacgatgac gacgaccacc
     4861 ggacgaacgc atctcgaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
     4921 gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat
     4981 gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta
     5041 ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta
     5101 aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt
     5161 ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac
     5221 attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg
     5281 gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc
     5341 tccctagcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat
     5401 tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg
     5461 ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat
     5521 agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta
     5581 tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa
     5641 gcatatttga gaagatgcgg ccagcaaaac taaggtacct gtatttagaa aaataaacaa
     5701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc atcacgtgct
     5761 ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa atagaaagta
     5821 aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg
     5881 gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat taatgccgaa
     5941 ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt tacattttac
     6001 ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg
     6061 taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt cttcattccg
     6121 taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat aaaaataaat
     6181 aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg cgtgtaagtt
     6241 acaggcaagc gatccgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     6301 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6361 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6421 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     6481 gagcagattg tactgagagt gcaccataga tcacgacatt actatatata taatatagga
     6541 agcatttaat agaacagcat cgtaatatat gtgtactttg cagttatgac gccagatggc
     6601 agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca atcttgatcc
     6661 ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa aaggagaggg
     6721 ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa
     6781 ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt
     6841 ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct
     6901 gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat
     6961 ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact tggttggcgt
     7021 gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg gcattgatat
     7081 cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg gtttgccagt
     7141 tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag cttcacagaa
     7201 acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg aacttttgga
     7261 ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct tacattttat
     7321 gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa atggcgttat
     7381 tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta acaaaatagc
     7441 aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat ttaagtattg
     7501 tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7561 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7621 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7681 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7741 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7801 cctaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8101 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8161 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8221 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8281 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8341 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8401 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8461 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8521 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8581 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8641 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8701 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc tgacttccgc
     8761 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     8821 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     8881 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     8941 acccgtcaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     9001 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     9061 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     9121 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     9181 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat
     9241 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct
     9301 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt
     9361 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta
     9421 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa
     9481 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg
     9541 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt
     9601 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta
     9661 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt
     9721 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta
     9781 aggttccttc acaaagatcc ggaccaaagc ggccatcgtg cctccccact cctgcagttc
     9841 gggggcatgg atgcgcggat agccgctgct ggtttcctgg atgccgacgg atttgcactg
     9901 ccggtagaac tccgcgaggt cgtccagcct caggcagcag ctgaaccaac tcgcgagggg
     9961 atcgagcccg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
    10021 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
    10081 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
    10141 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
    10201 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
    10261 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
    10321 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
    10381 acgacgagat cctcgccgtc gggcatccgc gccttgagcc tggcgaacag ttcggctggc
    10441 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
    10501 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
    10561 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
    10621 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
    10681 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
    10741 cgcgctgcct cgtcttggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
    10801 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
    10861 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
    10921 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
    10981 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
    11041 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc
    11101 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg
    11161 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
    11221 ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg
    11281 cagcgtgaga gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat
    11341 tgctggggca acacgtggag cggatcgggg attgtctttc ttcagctcgc tgatgatatg
    11401 ctgacgctca atgccgtttg gcctccgact aacgaaaatc ccgcatttgg acggctgatc
    11461 cgattggcac ggcggacggc gaatggcgga gcagacgctc gtccgggggc aatgagatat
    11521 gaaaaagcct gaactcaccg cgacgtatcg ggccctggcc agctagctag agtcgacctg
    11581 caggtccccg gggatcggtc ttgccttgct cgtcggtgat gtacttcacc agctccgcga
    11641 agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc
    11701 cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc
    11761 ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca
    11821 ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg
    11881 cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg
    11941 attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc
    12001 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
    12061 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
    12121 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
    12181 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
    12241 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
    12301 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
    12361 gcggaaaaga tccgtcgacc tgcaggcatg caagctctag cgattccaga cgtcccgaag
    12421 gcgtggcgcg gcttccccgt gccggagcaa tcgccctggg tgggttacac gacgcccctc
    12481 tatggcccgt actgacggac acaccgaagc cccggcggca accctcagcg gatgccccgg
    12541 ggcttcacgt tttcccaggt cagaagcg
//
