LOCUS       sequence-233756-        6240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF4_CRISPR resistant
FEATURES             Location/Qualifiers
     source          1..6240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(389..475)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     524..546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     538..555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     539..555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    571..670
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4121..4220)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4237..4256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4238..4256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4261..4277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4261..4277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4708..5499
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5592..6180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6081..6100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
      241 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      301 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      361 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      421 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
      481 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
      541 aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg actgatagtg
      601 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      661 aaagcaggct tcaccatgga cgccgtcaac gccttcaacc aggagctctt ttcgcttatg
      721 gatatgaaac ctcccatctc tagagccaag atgattctca tcactaaagc tgctattaaa
      781 gctattaagc tttataagca tgtagttcaa atagtagaaa agttcatcaa aaagtgtaaa
      841 ccagaataca aggttccggg attatatgta attgactcaa ttgtgcgaca gtctcgtcat
      901 cagtttggaa ctgataaaga tgtttttggg ccaagattct ctaaaaacat aactgccaca
      961 ttccaatatt tatatctttg tccatctgaa gataagagta aaatagttcg tgtgctgaac
     1021 ctttggcaaa aaaatggagt gttcaaaatt gaaattattc aacctctttt ggacatggca
     1081 gcgggaacca gtaatgcagc cccagtagca gaaaatgtta ccaataatga aggctcacct
     1141 ccacctccag taaaagtttc ttctgaacct cccacacaag ccactccaaa ctccgtgcct
     1201 gcagttcctc aattacccag ctctgatgct tttgctgctg tggctcaact cttccaaact
     1261 acacagggcc aacagcttca gcagatcctt cagacttttc aacagcctcc aaaaccacag
     1321 tctcctgccc ttgacaatgc tgtgatggct caggttcagg ctatcacagc tcagttaaag
     1381 acaactccta cacaaccatc tgaacaaaaa gctgctttcc ccccacctga acagaaaact
     1441 gcatttgaca agaagttgct tgatagattt gactatgatg atgagccaga agctgtggaa
     1501 gaatcaaaga aagaggatac cactgccgtc accacgacag cacctgctgc cgcagtaccc
     1561 cctgcaccca ccgccaccgt gcctgctgct gctgcacccg ctgctgcctc tcctcctcct
     1621 ccacaggcac catttggctt tcctggagat ggcatgcagc agcctgctta tacgcaacac
     1681 cagaatatgg atcaattcca acctcgtatg atgggaatac aacaggatcc aatgcaccat
     1741 caggttccac ttcctcctaa tggacaaatg ccaggatttg gacttcttcc tacacctcca
     1801 tttcctccca tggctcagcc tgtgattcct ccaactccac cagtgcagca gcctttccaa
     1861 gcttcttttc aggcacaaaa tgaaccactt acacagaagc cgcatcagca ggaaatggaa
     1921 gtagaacaac cttgtattca agaggttaag cgacatatgt ctgataacag aaagtcaaga
     1981 tctaggtcag catccaggtc accaaaaagg aggcgatcta gatctggttc tagatctcga
     2041 aggtctcggc atcgacgttc tcgatctcgg tccagggata gacgccgaca ttctccccga
     2101 tctcgatctc aagaaagacg ggatcgagaa aaagagagag aacgtcgaca aaaaggcctc
     2161 cctcaagtga aaccggaaac tgcaagtgtt tgcagtacta ccctctgggt ggggcagctg
     2221 gacaaaagaa ctactcagca ggatgttgcc agtctcttgg aagagtttgg tccaattgaa
     2281 tcaattaata tgattcctcc caggggttgt gcctatattg ttatggttca taggcaagat
     2341 gcctatcgtg ccctgcagaa actgagccga ggaaactata aagtgaacca gaaatccata
     2401 aagattgcct gggccttaaa caaaggaata aaggcagatt ataagcagta ttgggatgta
     2461 gaacttggtg ttacttatat tccatgggac aaagtcaagc ctgaggaact ggagagtttt
     2521 tgtgaaggag gaatgttgga cagtgacaca cttaacccag attggaaagg aattcctaag
     2581 aagcctgaaa atgaagttgc tcaaaatgga ggtgctgaaa cctcacacac agaaccagta
     2641 tcacccatac ctaaaccatt acctgtgcct gtccctccta ttcctgttcc tgcacctata
     2701 acagtgccac ctccacaggt cccaccacat caaccgggtc cacctgtagt tggtgctctc
     2761 cagccgcctg ctttcacgcc tcctctggga ataccgcctc caggctttgg tcctggtgtt
     2821 cctcctcccc ctcctcctcc accatttttg cgcccaggat tcaacccaat gcatttacca
     2881 ccaggttttc tgcctcctgg acccccacct cctataactc caccagtatc cattcctcct
     2941 cctcacactc caccaataag catcccaaac tctactatcg ctggtataaa tgaagacact
     3001 acaaaagact tatctattgg aaatcccatt ccaacagtgg tgtctggggc tagaggaaac
     3061 gccgagtctg gtgacagcgt gaaaatgtat ggctctgccg tgccacctgc tgcacccacg
     3121 aatctgccca cccctcctgt aacccagcct gtttcacttc ttggcactca aggagttgcc
     3181 cctggtcctg taattggact tcaggcacca tctactggtc ttcttggcgc ccggcccggt
     3241 ctcatcccac tccagcgccc tccaggaatg cccccacctc acttacagcg gttccctttg
     3301 atgccgcccc gtcccatgcc accgcacatg atgcacagag gcccaccgcc aggaccaggg
     3361 ggctttgcga tgcctccacc tcatggaatg aaaggtccct tcccaccgca tggccccttt
     3421 gttaggcctg gtggaatgcc agggctcggg ggcccagggc caggcccagg gggtcctgaa
     3481 gacagagacg gaaggcaaca gccgccgcag cagccacagc agcagccaca gccgcaggcg
     3541 ccccagcaac cacagcagca gcagcagcag cagccaccac catcacaaca gcctccacca
     3601 acacagcagc agccacagca gtttagaaat gataacaggc agcagttcaa ttcaggtaga
     3661 gaccaagaaa ggtttggaag aagatctttt ggaaataggg tggaaaatga ccgggaacgg
     3721 tatgggaacc gtaatgatga tagagataat agtaaccgtg acaggagaga gtggggaagg
     3781 aggagccctg accgggacag gcacagagac ttggaagaga gaaatagacg ctctagtggg
     3841 catcgagaca gagagagaga ttctagagat agagagtctc gtagagagaa ggaagaagcc
     3901 cgaggaaagg aaaagcctga ggtgacagac agggcaggtg gtaacaaaac cgttgaacct
     3961 cccattagcc aagtgggaaa tgtagacact gcttcagaac ttgagaaggg ggtgtctgag
     4021 gctgcagtcc taaagccttc tgaagagtta cctgctgagg ctacctcatc cgttgaaccc
     4081 gaaaaggatt ctggctcagc agcagaggct cctcgttaga acccagcttt cttgtacaaa
     4141 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     4201 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     4261 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     4321 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     4381 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     4441 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     4501 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     4561 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     4621 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     4681 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     4741 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     4801 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     4861 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     4921 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     4981 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     5041 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     5101 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     5161 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     5221 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     5281 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     5341 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     5401 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     5461 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     5521 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     5581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5701 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5941 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     6001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
//
