LOCUS       sequence-233768-        8637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gRNA vs Dmap1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8637)
  TITLE     Yusa CRISPR-Cas9 and mammalian expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
FEATURES             Location/Qualifiers
     source          1..8637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        197..395
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     350..370
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             413..593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    640..765
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1264..1497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1682..1726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2024..2141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2216..2464
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2216..2236
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2387..2406
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2592..3079
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2627..2650)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3012..3031
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3119..3715
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3119..3138)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3615..3635
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3734..3787
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3788..4489
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4549..4569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4967..4978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5430..5447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5436..5660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5686..5706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5686..5704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5725..5741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5725..5741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5738..5760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5749..5765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5773..5779,5780..5797,5798..5803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5818..5839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5956..5973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6127..6715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6207..6226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6886..7677,7678..7746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7509..7528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7747..7851)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7877..8332)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8014..8035)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8226..8245
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8459..8481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8473..8490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8474..8490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8500..8519
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8500..8518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
       61 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      121 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      181 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      241 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      301 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      361 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      421 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      481 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      541 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      601 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      661 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      721 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      781 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      841 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      901 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      961 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1021 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1081 agtaagacca ccgcacagca agcggccggc cgcgctgatc ttcagacctg gaggaggaga
     1141 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1201 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1261 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1321 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1381 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1441 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1501 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1561 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1621 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1681 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1741 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1801 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1861 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1921 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     1981 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2041 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2101 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2161 gcagagatcc agtttggtta gtaccgggcc ctacgcgtcc aaggtcgggc aggaagaggg
     2221 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     2281 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     2341 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     2401 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     2461 caccgttcgt agttattcac gatgtttaag agctatgctg gaaacagcat agcaagttta
     2521 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat
     2581 ccaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2641 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2701 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2761 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2821 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2881 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     2941 gctgggaagg ggtgggtccg ggggngggct caggggcggg gcgggcgccc gaaggtcctc
     3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3061 ctcatctccg ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat
     3121 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3181 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3241 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3301 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3361 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3421 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3481 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3541 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3601 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3661 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggcgg
     3721 cgggtccgga ggagagggca gaggaagtct cctaacatgc ggtgacgtgg aggagaatcc
     3781 tggcccaatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     3841 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     3901 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     3961 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     4021 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgagga
     4081 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     4141 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     4201 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     4261 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     4321 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     4381 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     4441 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattgag cggccgctca
     4501 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4561 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4621 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4681 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4741 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4801 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4861 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4921 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4981 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctacgcct
     5041 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcaggtac ctttaagacc
     5101 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5161 agggctaatt cactcccaaa gaagtcaaga tctgcttttt gcctgtactg ggtctctctg
     5221 gttagaccag agtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5281 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5341 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5401 atctctagca gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5461 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5521 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5581 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gtcaatagca ggcatgctgg
     5641 ggatgcggtg ggctctatgg gcggccgtta attaagcttt tgttcccttt agtgagggtt
     5701 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5761 cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg
     5821 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5881 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5941 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6001 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6061 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7861 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7921 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7981 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8041 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8101 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8161 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8221 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8281 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     8341 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8401 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8461 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     8521 gaattgacta gtacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttc
//
