LOCUS       sequence-233777-        6516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6516)
  TITLE     AAV expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-Syn-iCre
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..166
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(204..226)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     326..345
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(362..817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(499..520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     711..730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   892..1032
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   903..1032
                     /label=AAV2 ITR
     enhancer        1392..1694
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      2727..2736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2739..2759
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3795..3821
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3847..4435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3900..3920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4318..4329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4460..4581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4471..4490)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4525..4544
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4681..4810
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(4872..5460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4952..4971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5631..6422,6423..6491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6254..6273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6492..6516,1..80))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
       61 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
      121 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
      181 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      241 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      301 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
      361 aaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
      421 ttttttaacc aatagaccga aatcggcaaa atcccttata aatcaaaaga atagcccgag
      481 atagagttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      601 aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      721 gcgaaaggag cgggcgctaa ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      781 acacccgccg cgcttaatgc gccgctacag ggcgcgtact atggttgctt tgacgtatgc
      841 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc ccctgcaggc
      901 agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
      961 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
     1021 ctaggggttc ctgcggccgc ggtaccttaa ttaagggatg taattacgtc cctaacccgc
     1081 tagggggcag cagcgagccg aacggggctc cgctccggtc cggcgctaac cccgcatccc
     1141 cgagggggga tgtaattacg tccctaaccc gctagggggc agcagcgagc cgaacggggc
     1201 tccgctccgg tccggcgcta accccgcatc cccgaggggg gatgtaatta cgtccctaac
     1261 ccgctagggg gcagcagcga gccgaacggg gctccgctcc ggtccggcgc taaccccgca
     1321 tccccgaggg tcgactatta atagtaatca attacggggt cattagttca tagcccatat
     1381 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1441 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1501 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1561 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1621 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1681 atcgctatta ccattcagaa ccacggacag caccgaattc aatgtgtaat gtgtgctcaa
     1741 tatgctcatg tgtgttaccc tgagcttctg tgtctacata tatacctgga tgcctgtgtg
     1801 ttctgtgatg tacatatata ttctgtcttt ccttcctttt ctatttgtgt tattccatgt
     1861 gttctttcag attctcacca ccaagggcaa ggatatgtta actacccaag tgtccacctc
     1921 cgcctgtctg gtgatgttta cgccaccccc gtgctctttt ctttgcccga cagagttgtt
     1981 ataggagatg tctccccggg aacactgcag gaaggagaat ttctacattt atgttcccct
     2041 ctgagtgtgc ttctatcccc aaaatgcctt caaaggtgaa aatcaacact ggaaacccaa
     2101 gtatctggga agggcaagag tgtgtaagtg caagttagcc taaggaatag gaagaggttg
     2161 gtaaacaggg taggatcgtg ggagggagtt tcgttactac aggtccggac cctcaggaca
     2221 agaaccccac ccccactccc caaattgcgc atcccccgcc cccatcagag ggggagggga
     2281 agaggttgcg gcgcggcgca tgcgcactgt cggattcagc accgcggtca gagccttcgc
     2341 ctccgctgcc ggcgcgcacc accacctccc cagcaccaaa ggctgactga cgtcactcac
     2401 tagccctccc caaactcccc ttcctcgccg ccttggtcgc gtccatgctg ccgtgagtcc
     2461 agtcggaccg caccacgaga ggtgcaagat agggaggtgc gggcgcgacc atacgctctg
     2521 cggcggcaga gcctcggcgc tgcctcagtc tgcagcgggc agcagaggag tcgcgtcgtg
     2581 ccagagagca ccgccgtgct cctgagcccc tcgcgctccg cccccgcggc ccacagaccc
     2641 actgcccctt ggatcccctg ccccacgcca gagcctctgc taaccatgtt catgccttct
     2701 tctttttcct acaggagctc gccaccgcca ccatggtgcc caagaagaag aggaaagtct
     2761 ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc acctctgatg
     2821 aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct gaacacacct
     2881 ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg aacaacagga
     2941 aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg caagccagag
     3001 gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg cacaggagat
     3061 ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg agaatcagaa
     3121 aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa cgcactgact
     3181 ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc aggaacctgg
     3241 ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc agaatcagag
     3301 tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc aggaccaaga
     3361 ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc aagctggtgg
     3421 agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg ttctgccggg
     3481 tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc cgggccctgg
     3541 aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac tctgggcaga
     3601 gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccagggac atggccaggg
     3661 ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg aacattgtga
     3721 tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg ctcgaggatg
     3781 gggacagcgg cggataccct tacgacgtgc ctgactacgc ctgactgcag aacggccgtt
     3841 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4441 ttgcggccgt cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4501 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4561 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4621 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tagcggccgc
     4681 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4741 ccgggcgacc aaaggtcgcc cgacgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4801 tgcctgcagg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6481 tgaatactca tactcttcct ttttcaatat tattga
//
